Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer
Background: Clinically, Jian Pi Yang Zheng Xiao Zheng (JPYZXZ) has been used for many years to treat gastric cancer (GC); however, the underlying mechanism and targets are still undetermined. Methods: The active JPYZXZ components were assessed by Liquid chromatography combined with quadrupole-orbitr...
| Huvudupphovsmän: | , , , , , |
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| Materialtyp: | Artikel |
| Språk: | English |
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Elsevier
2024-03-01
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| Serie: | Pharmacological Research - Modern Chinese Medicine |
| Ämnen: | |
| Länkar: | http://www.sciencedirect.com/science/article/pii/S2667142524000241 |
| _version_ | 1827312330379100160 |
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| author | Yuan-jie Liu Jie-pin Li Ying Zhang Yi Yin Shen-lin Liu Xi Zou |
| author_facet | Yuan-jie Liu Jie-pin Li Ying Zhang Yi Yin Shen-lin Liu Xi Zou |
| author_sort | Yuan-jie Liu |
| collection | DOAJ |
| description | Background: Clinically, Jian Pi Yang Zheng Xiao Zheng (JPYZXZ) has been used for many years to treat gastric cancer (GC); however, the underlying mechanism and targets are still undetermined. Methods: The active JPYZXZ components were assessed by Liquid chromatography combined with quadrupole-orbitrap high-resolution mass spectrometry (LC-Q-orbitrap-HRMS). These findings were combined with the TCM Systematic Pharmacology Database and Analysis Platform (TCMSP) database to perform network pharmacological analysis. Using second-generation sequencing on nude mouse samples and single-cell sequencing on human GC tissue, we investigated the potential targets and mechanisms of action of JPYZXZ. A multi-center clinical trial (www.chictr.org.cn ChiCTR1900028147) validated our findings. Results: A total of 869 compounds were detected using LC-Q-orbitrap-HRMS analysis of JPYZXZ granules in order to examine the underlying mechanism of JPYZXZ in the treatment of GC. The network pharmacology analysis indicated that the potential mechanism of JPYZXZ in treating GC is associated with multiple molecular mechanisms and signaling pathways. The overlap between target genes was identified by second-generation sequencing and gene expression patterns obtained from single-cell transcriptome sequencing. We identified many crucial genes, including DZIP1, FSTL3, USP51, LHFPL6, and NREP. Subsequent investigations have consistently demonstrated that the upregulation of these genes is strongly associated with the development of an immunosuppressive microenvironment. JPYZXZ has been shown in clinical trials to reduce the number of immunosuppressive cell types in patients' peripheral blood, lower the levels of inflammatory factors, extend survival, and modify the immunosuppressive microenvironment. Conclusions: This investigation elucidated the positive impact of multi-component, multi-target, and multi-approach JPYZXZ intervention for GC treatment. |
| first_indexed | 2024-03-08T05:14:15Z |
| format | Article |
| id | doaj.art-822ec9d535ff43738b8457bb040cf064 |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-04-24T21:41:39Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-822ec9d535ff43738b8457bb040cf0642024-03-21T05:38:04ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252024-03-0110100379Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancerYuan-jie Liu0Jie-pin Li1Ying Zhang2Yi Yin3Shen-lin Liu4Xi Zou5Department of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China; No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Key Laboratory of Tumor System Biology of Traditional Chinese Medicine, Nanjing, Jiangsu 210029, ChinaDepartment of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China; No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Key Laboratory of Tumor System Biology of Traditional Chinese Medicine, Nanjing, Jiangsu 210029, ChinaDepartment of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China; No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaDepartment of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China; No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaDepartment of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China; No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Corresponding authors at: Department of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China.Department of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China; No. 1 Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Corresponding authors at: Department of Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China.Background: Clinically, Jian Pi Yang Zheng Xiao Zheng (JPYZXZ) has been used for many years to treat gastric cancer (GC); however, the underlying mechanism and targets are still undetermined. Methods: The active JPYZXZ components were assessed by Liquid chromatography combined with quadrupole-orbitrap high-resolution mass spectrometry (LC-Q-orbitrap-HRMS). These findings were combined with the TCM Systematic Pharmacology Database and Analysis Platform (TCMSP) database to perform network pharmacological analysis. Using second-generation sequencing on nude mouse samples and single-cell sequencing on human GC tissue, we investigated the potential targets and mechanisms of action of JPYZXZ. A multi-center clinical trial (www.chictr.org.cn ChiCTR1900028147) validated our findings. Results: A total of 869 compounds were detected using LC-Q-orbitrap-HRMS analysis of JPYZXZ granules in order to examine the underlying mechanism of JPYZXZ in the treatment of GC. The network pharmacology analysis indicated that the potential mechanism of JPYZXZ in treating GC is associated with multiple molecular mechanisms and signaling pathways. The overlap between target genes was identified by second-generation sequencing and gene expression patterns obtained from single-cell transcriptome sequencing. We identified many crucial genes, including DZIP1, FSTL3, USP51, LHFPL6, and NREP. Subsequent investigations have consistently demonstrated that the upregulation of these genes is strongly associated with the development of an immunosuppressive microenvironment. JPYZXZ has been shown in clinical trials to reduce the number of immunosuppressive cell types in patients' peripheral blood, lower the levels of inflammatory factors, extend survival, and modify the immunosuppressive microenvironment. Conclusions: This investigation elucidated the positive impact of multi-component, multi-target, and multi-approach JPYZXZ intervention for GC treatment.http://www.sciencedirect.com/science/article/pii/S2667142524000241Gastric cancerJian Pi Yang Zheng Xiao Zheng (JPYZXZ) granulesSingle-cell RNA sequencingSecond generation sequencingClinical trials |
| spellingShingle | Yuan-jie Liu Jie-pin Li Ying Zhang Yi Yin Shen-lin Liu Xi Zou Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer Pharmacological Research - Modern Chinese Medicine Gastric cancer Jian Pi Yang Zheng Xiao Zheng (JPYZXZ) granules Single-cell RNA sequencing Second generation sequencing Clinical trials |
| title | Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer |
| title_full | Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer |
| title_fullStr | Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer |
| title_full_unstemmed | Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer |
| title_short | Comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of Jian Pi Yang Zheng Xiao Zheng in the treatment of gastric cancer |
| title_sort | comprehensive analysis to evaluate the molecular biological mechanism and clinical efficacy of jian pi yang zheng xiao zheng in the treatment of gastric cancer |
| topic | Gastric cancer Jian Pi Yang Zheng Xiao Zheng (JPYZXZ) granules Single-cell RNA sequencing Second generation sequencing Clinical trials |
| url | http://www.sciencedirect.com/science/article/pii/S2667142524000241 |
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