Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity
In order to obtain homogeneous <i>Sanghuangporus vaninii</i> polysaccharides with antioxidant and anti-inflammatory activities, a response surface method (RSM) was used to compare the polysaccharide extraction rate of hot water extraction and ultrasonic-assisted extraction from <i>...
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MDPI AG
2023-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/16/6081 |
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| author | Jinze Liu Jinyue Song Fusheng Gao Weijia Chen Ying Zong Jianming Li Zhongmei He Rui Du |
| author_facet | Jinze Liu Jinyue Song Fusheng Gao Weijia Chen Ying Zong Jianming Li Zhongmei He Rui Du |
| author_sort | Jinze Liu |
| collection | DOAJ |
| description | In order to obtain homogeneous <i>Sanghuangporus vaninii</i> polysaccharides with antioxidant and anti-inflammatory activities, a response surface method (RSM) was used to compare the polysaccharide extraction rate of hot water extraction and ultrasonic-assisted extraction from <i>Sanghuangporus vaninii</i>. The optimal conditions for ultrasonic-assisted extraction were determined as follows: an extraction temperature of 60 °C, an extraction time of 60 min, a solid–liquid ratio of 40 g/mL, and an ultrasonic power of 70 W. An SVP (<i>Sanghuangporus vaninii</i> polysaccharides) extraction rate of 1.41% was achieved. Five homogeneous monosaccharides were obtained by gradient ethanol precipitation with diethylaminoethyl–cellulose (DEAE) and SephadexG-100 separation and purification. The five polysaccharides were characterized by high performance liquid chromatography, the ultraviolet spectrum, the Fourier transform infrared spectrum, TG (thermogravimetric analysis), the Zeta potential, and SEM (scanning electron microscopy). The five polysaccharides had certain levels of antioxidant activity in vitro. In addition, we the investigated the anti-inflammatory effects of polysaccharides derived from <i>Sanghuangporus vaninii</i> on lipopolysaccharide (LPS)-induced RAW 264.7 cells and Kupffer cells. Further, we found that SVP-60 significantly inhibited the levels of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-induced RAW 264.7 cells and promoted the level of the anti-inflammatory cytokine IL-10 in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Our study provides theoretical support for the potential application of <i>Sanghuangporus vaninii</i> in the field of antioxidant and anti-inflammatory activities in vitro. |
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| last_indexed | 2024-03-10T23:41:33Z |
| publishDate | 2023-08-01 |
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| series | Molecules |
| spelling | doaj.art-8232ea96f8124d04a238eebe860c1d302023-11-19T02:24:34ZengMDPI AGMolecules1420-30492023-08-012816608110.3390/molecules28166081Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory ActivityJinze Liu0Jinyue Song1Fusheng Gao2Weijia Chen3Ying Zong4Jianming Li5Zhongmei He6Rui Du7College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, ChinaIn order to obtain homogeneous <i>Sanghuangporus vaninii</i> polysaccharides with antioxidant and anti-inflammatory activities, a response surface method (RSM) was used to compare the polysaccharide extraction rate of hot water extraction and ultrasonic-assisted extraction from <i>Sanghuangporus vaninii</i>. The optimal conditions for ultrasonic-assisted extraction were determined as follows: an extraction temperature of 60 °C, an extraction time of 60 min, a solid–liquid ratio of 40 g/mL, and an ultrasonic power of 70 W. An SVP (<i>Sanghuangporus vaninii</i> polysaccharides) extraction rate of 1.41% was achieved. Five homogeneous monosaccharides were obtained by gradient ethanol precipitation with diethylaminoethyl–cellulose (DEAE) and SephadexG-100 separation and purification. The five polysaccharides were characterized by high performance liquid chromatography, the ultraviolet spectrum, the Fourier transform infrared spectrum, TG (thermogravimetric analysis), the Zeta potential, and SEM (scanning electron microscopy). The five polysaccharides had certain levels of antioxidant activity in vitro. In addition, we the investigated the anti-inflammatory effects of polysaccharides derived from <i>Sanghuangporus vaninii</i> on lipopolysaccharide (LPS)-induced RAW 264.7 cells and Kupffer cells. Further, we found that SVP-60 significantly inhibited the levels of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-induced RAW 264.7 cells and promoted the level of the anti-inflammatory cytokine IL-10 in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Our study provides theoretical support for the potential application of <i>Sanghuangporus vaninii</i> in the field of antioxidant and anti-inflammatory activities in vitro.https://www.mdpi.com/1420-3049/28/16/6081<i>Sanghuangporus vaninii</i>polysaccharideultrasonic extractionoptimizationstructure analysisantioxidant activity |
| spellingShingle | Jinze Liu Jinyue Song Fusheng Gao Weijia Chen Ying Zong Jianming Li Zhongmei He Rui Du Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity Molecules <i>Sanghuangporus vaninii</i> polysaccharide ultrasonic extraction optimization structure analysis antioxidant activity |
| title | Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity |
| title_full | Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity |
| title_fullStr | Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity |
| title_full_unstemmed | Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity |
| title_short | Extraction, Purification, and Structural Characterization of Polysaccharides from <i>Sanghuangporus vaninii</i> with Anti-Inflammatory Activity |
| title_sort | extraction purification and structural characterization of polysaccharides from i sanghuangporus vaninii i with anti inflammatory activity |
| topic | <i>Sanghuangporus vaninii</i> polysaccharide ultrasonic extraction optimization structure analysis antioxidant activity |
| url | https://www.mdpi.com/1420-3049/28/16/6081 |
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