Serology of paracoccidioidomycosis due to Paracoccidioides lutzii.
Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply t...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-07-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC4102441?pdf=render |
| _version_ | 1818557995921965056 |
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| author | Gregory Gegembauer Leticia Mendes Araujo Edy Firmina Pereira Anderson Messias Rodrigues Anamaria Mello Miranda Paniago Rosane Christine Hahn Zoilo Pires de Camargo |
| author_facet | Gregory Gegembauer Leticia Mendes Araujo Edy Firmina Pereira Anderson Messias Rodrigues Anamaria Mello Miranda Paniago Rosane Christine Hahn Zoilo Pires de Camargo |
| author_sort | Gregory Gegembauer |
| collection | DOAJ |
| description | Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests. |
| first_indexed | 2024-12-14T00:07:01Z |
| format | Article |
| id | doaj.art-8234ad20508e43fabe9d60d5c378f923 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-14T00:07:01Z |
| publishDate | 2014-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-8234ad20508e43fabe9d60d5c378f9232022-12-21T23:25:59ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352014-07-0187e298610.1371/journal.pntd.0002986Serology of paracoccidioidomycosis due to Paracoccidioides lutzii.Gregory GegembauerLeticia Mendes AraujoEdy Firmina PereiraAnderson Messias RodriguesAnamaria Mello Miranda PaniagoRosane Christine HahnZoilo Pires de CamargoParacoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.http://europepmc.org/articles/PMC4102441?pdf=render |
| spellingShingle | Gregory Gegembauer Leticia Mendes Araujo Edy Firmina Pereira Anderson Messias Rodrigues Anamaria Mello Miranda Paniago Rosane Christine Hahn Zoilo Pires de Camargo Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. PLoS Neglected Tropical Diseases |
| title | Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. |
| title_full | Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. |
| title_fullStr | Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. |
| title_full_unstemmed | Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. |
| title_short | Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. |
| title_sort | serology of paracoccidioidomycosis due to paracoccidioides lutzii |
| url | http://europepmc.org/articles/PMC4102441?pdf=render |
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