Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production
In Cuba, a biofertilizer has been developed based on a nitrogen fixer Azotobacter chroococcum strain. This work aimed to optimize the culture medium and the fermentation conditions for industrial scale. First, tests in the shaker were carried out and then in a fermenter. In each case an experimental...
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2020-09-01
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| Series: | Biotecnología Vegetal |
| Subjects: | |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/671/html |
| _version_ | 1818394870790750208 |
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| author | María Cristina Pérez Peñaranda Julio Oramas García Emilio Arcadio Sotolongo Valdés Yoandra Román Tabio Héctor López Rodriguez |
| author_facet | María Cristina Pérez Peñaranda Julio Oramas García Emilio Arcadio Sotolongo Valdés Yoandra Román Tabio Héctor López Rodriguez |
| author_sort | María Cristina Pérez Peñaranda |
| collection | DOAJ |
| description | In Cuba, a biofertilizer has been developed based on a nitrogen fixer Azotobacter chroococcum strain. This work aimed to optimize the culture medium and the fermentation conditions for industrial scale. First, tests in the shaker were carried out and then in a fermenter. In each case an experimental validation was performed. For the optimization of the culture medium, a central composite design was used to adjust response surfaces. A 23 factorial design with equidistant star points and six repetitions in the center of the plan. A total of 19 experimental runs were performed, with three repetitions determined. As a response variable, cell growth was determined by optical density λ = 540 nm (OD540). To optimize microbial growth based on agitation speed and air flow, a 7.5, liter with a factorial design 22 was used, taking OD540 as the response variable. With the means of optimized cultivation a growth of 2-4 x 109 UFC ml-1 was obtained that allow to increase the content of bacterial biomass together to the use of a speed of agitation and good flow of air. The new conditions allowed to reduce the time of fermentation at 10 hours, less than 20 to 22 hours used in the production scheme. In addition, 40%, 50% and 20% respectively, of carbon, nitrogen and phosphorus sources were reduced. |
| first_indexed | 2024-12-14T06:08:05Z |
| format | Article |
| id | doaj.art-8235fda90c4f40558ed58e72995449c9 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-14T06:08:05Z |
| publishDate | 2020-09-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-8235fda90c4f40558ed58e72995449c92022-12-21T23:14:14ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472020-09-01203189201Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers productionMaría Cristina Pérez Peñaranda0https://orcid.org/0000-0001-6001-017XJulio Oramas García 1https://orcid.org/0000-0001-7230-874XEmilio Arcadio Sotolongo Valdés 2https://orcid.org/0000-0001-7839-3148Yoandra Román Tabio3https://orcid.org/0000-0001-9414-1928Héctor López Rodriguez4 Unidad de Desarrollo–Innovación del Grupo Empresarial Laboratorios Biológicos Farmacéuticos (LABIOFAM). Ave. Independencia km 16 ½. Boyeros. La Habana. Cuba.; Cuba Unidad de Desarrollo–Innovación del Grupo Empresarial Laboratorios Biológicos Farmacéuticos (LABIOFAM). Ave. Independencia km 16 ½. Boyeros. La Habana. Cuba.; Cuba Facultad de Ingeniería Química, Universidad Tecnológica de La Habana. Calle 114, No. 11901, entre Ciclovía y Rotonda. Marianao. La Habana. Cuba.; CubaUnidad de Desarrollo–Innovación del Grupo Empresarial Laboratorios Biológicos Farmacéuticos (LABIOFAM). Ave. Independencia km 16 ½. Boyeros. La Habana. Cuba.; Cuba Facultad de Ingeniería Química, Universidad Tecnológica de La Habana. Calle 114, No. 11901, entre Ciclovía y Rotonda. Marianao. La Habana. Cuba.; Cuba In Cuba, a biofertilizer has been developed based on a nitrogen fixer Azotobacter chroococcum strain. This work aimed to optimize the culture medium and the fermentation conditions for industrial scale. First, tests in the shaker were carried out and then in a fermenter. In each case an experimental validation was performed. For the optimization of the culture medium, a central composite design was used to adjust response surfaces. A 23 factorial design with equidistant star points and six repetitions in the center of the plan. A total of 19 experimental runs were performed, with three repetitions determined. As a response variable, cell growth was determined by optical density λ = 540 nm (OD540). To optimize microbial growth based on agitation speed and air flow, a 7.5, liter with a factorial design 22 was used, taking OD540 as the response variable. With the means of optimized cultivation a growth of 2-4 x 109 UFC ml-1 was obtained that allow to increase the content of bacterial biomass together to the use of a speed of agitation and good flow of air. The new conditions allowed to reduce the time of fermentation at 10 hours, less than 20 to 22 hours used in the production scheme. In addition, 40%, 50% and 20% respectively, of carbon, nitrogen and phosphorus sources were reduced.https://revista.ibp.co.cu/index.php/BV/article/view/671/htmlbioproductcentral composite designresponse surface methodology |
| spellingShingle | María Cristina Pérez Peñaranda Julio Oramas García Emilio Arcadio Sotolongo Valdés Yoandra Román Tabio Héctor López Rodriguez Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production Biotecnología Vegetal bioproduct central composite design response surface methodology |
| title | Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production |
| title_full | Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production |
| title_fullStr | Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production |
| title_full_unstemmed | Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production |
| title_short | Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production |
| title_sort | optimization of the culture medium and fermentation conditions for azotobacter chroococcum based biofertilizers production |
| topic | bioproduct central composite design response surface methodology |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/671/html |
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