MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination
Rutin is a flavonoid glycoside compound, which is mainly transported via the blood circulation system in the human body. The monitoring of the blood concentration of rutin is of great significance in many fields such as pharmacology and pharmacokinetics. In this work, a biosensor based on multi-wall...
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MDPI AG
2022-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/8/632 |
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| author | Xin-Yan Song Xin Meng Bao-Lin Xiao Yang-Yang Li Xin-Xin Ma Ali Akbar Moosavi-Movahedi Jun Hong |
| author_facet | Xin-Yan Song Xin Meng Bao-Lin Xiao Yang-Yang Li Xin-Xin Ma Ali Akbar Moosavi-Movahedi Jun Hong |
| author_sort | Xin-Yan Song |
| collection | DOAJ |
| description | Rutin is a flavonoid glycoside compound, which is mainly transported via the blood circulation system in the human body. The monitoring of the blood concentration of rutin is of great significance in many fields such as pharmacology and pharmacokinetics. In this work, a biosensor based on multi-walled carbon nanotubes (MWCNTs), cetyltrimethylammonium bromide (CTAB), hydroxyl fullerenes (HFs), and laccase (Lac) nanocomposite-modified glassy carbon electrodes was constructed. The modified materials were characterized with a transmission electron microscope (TEM), cyclic voltammograms (CV), and electrochemical impedance spectroscopy (EIS). CTAB is used to disperse MWCNTs and improve hydrophilicity and biocompatibility of MWCNTs, while the use of Lac can enhance the oxidation of catechol structure in rutin, thus significantly improving the sensitivity and selectivity of the modified electrode. Linear sweep voltammetry (LSV) studies showed that the determination linear ranges of rutin were 0.1 µmol L<sup>−1</sup> to 2 µmol L<sup>−1</sup> and 2 µmol L<sup>−1</sup> to 11 µmol L<sup>−1</sup>, with the determination limits of 30 nmol L<sup>−1</sup> and 95.5 nmol L<sup>−1</sup>, respectively. The proposed biosensor can be used to detect rutin tablets and serum samples with high recovery, which indicates a good accuracy of this method, and the results are consistent with those measured by the traditional ultra-high performance liquid chromatography (UHPLC) method. Hence, this biosensor has potential practical application value in rutin drug quality testing and clinical blood drug concentration monitoring. |
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| language | English |
| last_indexed | 2024-03-09T09:59:34Z |
| publishDate | 2022-08-01 |
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| series | Biosensors |
| spelling | doaj.art-82366875539c424c95e044fe5cd239052023-12-01T23:30:24ZengMDPI AGBiosensors2079-63742022-08-0112863210.3390/bios12080632MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin DeterminationXin-Yan Song0Xin Meng1Bao-Lin Xiao2Yang-Yang Li3Xin-Xin Ma4Ali Akbar Moosavi-Movahedi5Jun Hong6School of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaInstitute of Biochemistry and Biophysics, University of Tehran, Tehran 1417614418, IranSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaRutin is a flavonoid glycoside compound, which is mainly transported via the blood circulation system in the human body. The monitoring of the blood concentration of rutin is of great significance in many fields such as pharmacology and pharmacokinetics. In this work, a biosensor based on multi-walled carbon nanotubes (MWCNTs), cetyltrimethylammonium bromide (CTAB), hydroxyl fullerenes (HFs), and laccase (Lac) nanocomposite-modified glassy carbon electrodes was constructed. The modified materials were characterized with a transmission electron microscope (TEM), cyclic voltammograms (CV), and electrochemical impedance spectroscopy (EIS). CTAB is used to disperse MWCNTs and improve hydrophilicity and biocompatibility of MWCNTs, while the use of Lac can enhance the oxidation of catechol structure in rutin, thus significantly improving the sensitivity and selectivity of the modified electrode. Linear sweep voltammetry (LSV) studies showed that the determination linear ranges of rutin were 0.1 µmol L<sup>−1</sup> to 2 µmol L<sup>−1</sup> and 2 µmol L<sup>−1</sup> to 11 µmol L<sup>−1</sup>, with the determination limits of 30 nmol L<sup>−1</sup> and 95.5 nmol L<sup>−1</sup>, respectively. The proposed biosensor can be used to detect rutin tablets and serum samples with high recovery, which indicates a good accuracy of this method, and the results are consistent with those measured by the traditional ultra-high performance liquid chromatography (UHPLC) method. Hence, this biosensor has potential practical application value in rutin drug quality testing and clinical blood drug concentration monitoring.https://www.mdpi.com/2079-6374/12/8/632biosensorlaccasehydroxyl fullerenesmulti-walled carbon nanotubesrutin determination |
| spellingShingle | Xin-Yan Song Xin Meng Bao-Lin Xiao Yang-Yang Li Xin-Xin Ma Ali Akbar Moosavi-Movahedi Jun Hong MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination Biosensors biosensor laccase hydroxyl fullerenes multi-walled carbon nanotubes rutin determination |
| title | MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination |
| title_full | MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination |
| title_fullStr | MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination |
| title_full_unstemmed | MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination |
| title_short | MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination |
| title_sort | mwcnts ctab and hfs lac nanocomposite modified glassy carbon electrode for rutin determination |
| topic | biosensor laccase hydroxyl fullerenes multi-walled carbon nanotubes rutin determination |
| url | https://www.mdpi.com/2079-6374/12/8/632 |
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