Continuous Flow Labeling and In-Line Magnetic Separation of Cells
There is an identified need for point-of-care diagnostic systems for detecting and counting specific rare types of circulating cells in blood. By adequately labeling such cells with immunomagnetic beads and quantum dots, they can be efficiently collected magnetically for quantification using fluores...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Magnetochemistry |
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| Online Access: | https://www.mdpi.com/2312-7481/8/1/5 |
| _version_ | 1827664692407959552 |
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| author | Zhixi Qian Thomas R. Hanley Lisa M. Reece James F. Leary Eugene D. Boland Paul Todd |
| author_facet | Zhixi Qian Thomas R. Hanley Lisa M. Reece James F. Leary Eugene D. Boland Paul Todd |
| author_sort | Zhixi Qian |
| collection | DOAJ |
| description | There is an identified need for point-of-care diagnostic systems for detecting and counting specific rare types of circulating cells in blood. By adequately labeling such cells with immunomagnetic beads and quantum dots, they can be efficiently collected magnetically for quantification using fluorescence methods. Automation of this process requires adequate mixing of the labeling materials with blood samples. A static mixing device can be employed to improve cell labeling efficiency and eliminate error-prone laboratory operations. Computational fluid dynamics (CFD) were utilized to simulate the flow of a labeling-materials/blood mixture through a 20-stage in-line static mixer of the interfacial-surface-generator type. Optimal fluid mixing conditions were identified and tested in a magnetic bead/tumor cell model, and it was found that labeled cells could be produced at 1.0 mL/min flow rate and fed directly into an in-line magnetic trap. The trap design consists of a dual flow channel with three bends and a permanent magnet positioned at the outer curve of each bend. The capture of labeled cells in the device was simulated using CFD, finite-element analysis and magnetophoretic mobility distributions of labeled cells. Testing with cultured CRL14777 human melanoma cells labeled with anti-CD146 1.5 μm diameter beads indicated that 90 ± 10% are captured at the first stage, and these cells can be captured when present in whole blood. Both in-line devices were demonstrated to function separately and together as predicted. |
| first_indexed | 2024-03-10T01:05:30Z |
| format | Article |
| id | doaj.art-8236aefd9aba46a8a67bfc6acee7df00 |
| institution | Directory Open Access Journal |
| issn | 2312-7481 |
| language | English |
| last_indexed | 2024-03-10T01:05:30Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Magnetochemistry |
| spelling | doaj.art-8236aefd9aba46a8a67bfc6acee7df002023-11-23T14:27:05ZengMDPI AGMagnetochemistry2312-74812021-12-0181510.3390/magnetochemistry8010005Continuous Flow Labeling and In-Line Magnetic Separation of CellsZhixi Qian0Thomas R. Hanley1Lisa M. Reece2James F. Leary3Eugene D. Boland4Paul Todd5Department of Chemical Engineering, Auburn University, Auburn, AL 36849, USADepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USABirck Nanotechnology Center, Purdue University, W. Lafayette, IN 47907-2057, USABirck Nanotechnology Center, Purdue University, W. Lafayette, IN 47907-2057, USATechshot, Inc., Greenville, IN 47124, USATechshot, Inc., Greenville, IN 47124, USAThere is an identified need for point-of-care diagnostic systems for detecting and counting specific rare types of circulating cells in blood. By adequately labeling such cells with immunomagnetic beads and quantum dots, they can be efficiently collected magnetically for quantification using fluorescence methods. Automation of this process requires adequate mixing of the labeling materials with blood samples. A static mixing device can be employed to improve cell labeling efficiency and eliminate error-prone laboratory operations. Computational fluid dynamics (CFD) were utilized to simulate the flow of a labeling-materials/blood mixture through a 20-stage in-line static mixer of the interfacial-surface-generator type. Optimal fluid mixing conditions were identified and tested in a magnetic bead/tumor cell model, and it was found that labeled cells could be produced at 1.0 mL/min flow rate and fed directly into an in-line magnetic trap. The trap design consists of a dual flow channel with three bends and a permanent magnet positioned at the outer curve of each bend. The capture of labeled cells in the device was simulated using CFD, finite-element analysis and magnetophoretic mobility distributions of labeled cells. Testing with cultured CRL14777 human melanoma cells labeled with anti-CD146 1.5 μm diameter beads indicated that 90 ± 10% are captured at the first stage, and these cells can be captured when present in whole blood. Both in-line devices were demonstrated to function separately and together as predicted.https://www.mdpi.com/2312-7481/8/1/5static mixercell labelingcell sedimentationFLUENT simulationcomputational fluid dynamicsmagnetic microparticles |
| spellingShingle | Zhixi Qian Thomas R. Hanley Lisa M. Reece James F. Leary Eugene D. Boland Paul Todd Continuous Flow Labeling and In-Line Magnetic Separation of Cells Magnetochemistry static mixer cell labeling cell sedimentation FLUENT simulation computational fluid dynamics magnetic microparticles |
| title | Continuous Flow Labeling and In-Line Magnetic Separation of Cells |
| title_full | Continuous Flow Labeling and In-Line Magnetic Separation of Cells |
| title_fullStr | Continuous Flow Labeling and In-Line Magnetic Separation of Cells |
| title_full_unstemmed | Continuous Flow Labeling and In-Line Magnetic Separation of Cells |
| title_short | Continuous Flow Labeling and In-Line Magnetic Separation of Cells |
| title_sort | continuous flow labeling and in line magnetic separation of cells |
| topic | static mixer cell labeling cell sedimentation FLUENT simulation computational fluid dynamics magnetic microparticles |
| url | https://www.mdpi.com/2312-7481/8/1/5 |
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