Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells
Selenium nanoparticles (SeNPs) have gained extensive attention for their excellent biological activity and low toxicity. However, SeNPs are extremely liable to aggregate into non-bioactive or gray elemental selenium, which limits their application in the biomedicine field. This study aimed to prepar...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.987807/full |
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| author | Xiong Gao Xiong Gao Xiong Gao Yanting Yao Xujie Chen Xiaorong Lin Xiaobing Yang Chi-Tang Ho Bin Li Bin Li Zhongzheng Chen Zhongzheng Chen |
| author_facet | Xiong Gao Xiong Gao Xiong Gao Yanting Yao Xujie Chen Xiaorong Lin Xiaobing Yang Chi-Tang Ho Bin Li Bin Li Zhongzheng Chen Zhongzheng Chen |
| author_sort | Xiong Gao |
| collection | DOAJ |
| description | Selenium nanoparticles (SeNPs) have gained extensive attention for their excellent biological activity and low toxicity. However, SeNPs are extremely liable to aggregate into non-bioactive or gray elemental selenium, which limits their application in the biomedicine field. This study aimed to prepare stable SeNPs by using lentinan (LNT) as a template and evaluate its anti-colon cancer activity. The average particle diameter of obtained lentinan-selenium nanoparticles (LNT-SeNPs) was approximately 59 nm and presented zero-valent, amorphous, and spherical structures. The monodisperse SeNPs were stabilized by LNT through hydrogen bonding interactions. LNT-SeNPs solution remained highly stable at 4°C for at least 8 weeks. The stability of LNT-SeNPs solution sharply decreased under high temperature and strong acidic conditions. LNT-SeNPs showed no obvious cytotoxic effect on normal cells (IEC-6) but significantly inhibited the proliferation of five colon cancer cells (HCT-116, HT-29, Caco-2, SW620, and CT26). Among them, LNT-SeNPs exhibited the highest sensitivity toward HCT-116 cells with an IC50 value of 7.65 μM. Also, LNT-SeNPs displayed better cancer cell selectivity than sodium selenite and selenomethionine. Moreover, LNT-SeNPs promoted apoptosis of HCT-116 cells through activating mitochondria-mediated apoptotic pathway. Meanwhile, LNT-SeNPs induced cell cycle arrest at G0/G1 phase in HCT-116 cells via modulation of cell cycle regulatory proteins. The results of this study indicated that LNT-SeNPs possessed strong potential application in the treatment of colorectal cancer (CRC). |
| first_indexed | 2024-12-11T00:44:12Z |
| format | Article |
| id | doaj.art-823b0ff2c7814ecb908e466c24b3356b |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T00:44:12Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-823b0ff2c7814ecb908e466c24b3356b2022-12-22T01:26:50ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-08-01910.3389/fnut.2022.987807987807Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cellsXiong Gao0Xiong Gao1Xiong Gao2Yanting Yao3Xujie Chen4Xiaorong Lin5Xiaobing Yang6Chi-Tang Ho7Bin Li8Bin Li9Zhongzheng Chen10Zhongzheng Chen11State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaDepartment of Food Science, Rutgers University, New Brunswick, NJ, United StatesCollege of Food Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, South China Agricultural University, Guangzhou, ChinaCollege of Food Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, South China Agricultural University, Guangzhou, ChinaSelenium nanoparticles (SeNPs) have gained extensive attention for their excellent biological activity and low toxicity. However, SeNPs are extremely liable to aggregate into non-bioactive or gray elemental selenium, which limits their application in the biomedicine field. This study aimed to prepare stable SeNPs by using lentinan (LNT) as a template and evaluate its anti-colon cancer activity. The average particle diameter of obtained lentinan-selenium nanoparticles (LNT-SeNPs) was approximately 59 nm and presented zero-valent, amorphous, and spherical structures. The monodisperse SeNPs were stabilized by LNT through hydrogen bonding interactions. LNT-SeNPs solution remained highly stable at 4°C for at least 8 weeks. The stability of LNT-SeNPs solution sharply decreased under high temperature and strong acidic conditions. LNT-SeNPs showed no obvious cytotoxic effect on normal cells (IEC-6) but significantly inhibited the proliferation of five colon cancer cells (HCT-116, HT-29, Caco-2, SW620, and CT26). Among them, LNT-SeNPs exhibited the highest sensitivity toward HCT-116 cells with an IC50 value of 7.65 μM. Also, LNT-SeNPs displayed better cancer cell selectivity than sodium selenite and selenomethionine. Moreover, LNT-SeNPs promoted apoptosis of HCT-116 cells through activating mitochondria-mediated apoptotic pathway. Meanwhile, LNT-SeNPs induced cell cycle arrest at G0/G1 phase in HCT-116 cells via modulation of cell cycle regulatory proteins. The results of this study indicated that LNT-SeNPs possessed strong potential application in the treatment of colorectal cancer (CRC).https://www.frontiersin.org/articles/10.3389/fnut.2022.987807/fulllentinanselenium nanoparticlescharacterizationstabilityHCT-116apoptosis |
| spellingShingle | Xiong Gao Xiong Gao Xiong Gao Yanting Yao Xujie Chen Xiaorong Lin Xiaobing Yang Chi-Tang Ho Bin Li Bin Li Zhongzheng Chen Zhongzheng Chen Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells Frontiers in Nutrition lentinan selenium nanoparticles characterization stability HCT-116 apoptosis |
| title | Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells |
| title_full | Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells |
| title_fullStr | Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells |
| title_full_unstemmed | Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells |
| title_short | Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells |
| title_sort | lentinan functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma hct 116 cells |
| topic | lentinan selenium nanoparticles characterization stability HCT-116 apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.987807/full |
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