Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue
Background: Triethylene glycol dimethacrylate (TEGDMA) monomers released from resin matrix are toxic to dental pulp cells, induce apoptosis, oxidative stress and decrease viability. Recently, mitochondrial complex I (CI) was identified as a potential target of TEGDMA. In isolated mitochondria suppor...
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| author | Krisztina Mikulás Timea Komlódi Anna Földes Gergely Sváb Gergő Horváth Ádám Miklós Nagy Attila Ambrus Szabolcs Gyulai-Gaál István Gera Péter Hermann Gábor Varga László Tretter |
| author_facet | Krisztina Mikulás Timea Komlódi Anna Földes Gergely Sváb Gergő Horváth Ádám Miklós Nagy Attila Ambrus Szabolcs Gyulai-Gaál István Gera Péter Hermann Gábor Varga László Tretter |
| author_sort | Krisztina Mikulás |
| collection | DOAJ |
| description | Background: Triethylene glycol dimethacrylate (TEGDMA) monomers released from resin matrix are toxic to dental pulp cells, induce apoptosis, oxidative stress and decrease viability. Recently, mitochondrial complex I (CI) was identified as a potential target of TEGDMA. In isolated mitochondria supported by CI, substrates oxidation and ATP synthesis were inhibited, reactive oxygen species production was stimulated. Contrary to that, respiratory Complex II was not impaired by TEGDMA. The beneficial effects of electron carrier compound methylene blue (MB) are proven in many disease models where mitochondrial involvement has been detected. In the present study, the bioenergetic effects of MB on TEGDMA-treated isolated mitochondria and on human dental pulp stem cells (DPSC) were analyzed. Methods: Isolated mitochondria and DPSC were acutely exposed to low millimolar concentrations of TEGDMA and 2 μM concentration of MB. Mitochondrial and cellular respiration and glycolytic flux were measured by high resolution respirometry and by Seahorse XF extracellular analyzer. Mitochondrial membrane potential was measured fluorimetrically. Results: MB partially restored the mitochondrial oxidation, rescued membrane potential in isolated mitochondria and significantly increased the impaired cellular O<sub>2</sub> consumption in the presence of TEGDMA. Conclusion: MB is able to protect against TEGDMA-induced CI damage, and might provide protective effects in resin monomer exposed cells. |
| first_indexed | 2024-03-10T17:51:55Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T17:51:55Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-823e525040584a83b1b36b9294fd494d2023-11-20T09:19:55ZengMDPI AGMaterials1996-19442020-08-011316347210.3390/ma13163472Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene BlueKrisztina Mikulás0Timea Komlódi1Anna Földes2Gergely Sváb3Gergő Horváth4Ádám Miklós Nagy5Attila Ambrus6Szabolcs Gyulai-Gaál7István Gera8Péter Hermann9Gábor Varga10László Tretter11Department of Prosthodontics, Semmelweis University, 1088 Budapest, HungaryDepartment of Medical Biochemistry, MTA-SE, Laboratory of Neurobiochemistry, Semmelweis University, 1094 Budapest, HungaryDepartment of Oral Biology, Semmelweis University, 1089 Budapest, HungaryDepartment of Medical Biochemistry, MTA-SE, Laboratory of Neurobiochemistry, Semmelweis University, 1094 Budapest, HungaryDepartment of Medical Biochemistry, MTA-SE, Laboratory of Neurobiochemistry, Semmelweis University, 1094 Budapest, HungaryDepartment of Medical Biochemistry, MTA-SE, Laboratory of Neurobiochemistry, Semmelweis University, 1094 Budapest, HungaryDepartment of Medical Biochemistry, MTA-SE, Laboratory of Neurobiochemistry, Semmelweis University, 1094 Budapest, HungaryDepartment of Oral Diagnostics, Semmelweis University, 1088 Budapest, HungaryDepartment of Periodontology, Semmelweis University, 1088 Budapest, HungaryDepartment of Prosthodontics, Semmelweis University, 1088 Budapest, HungaryDepartment of Oral Biology, Semmelweis University, 1089 Budapest, HungaryDepartment of Medical Biochemistry, MTA-SE, Laboratory of Neurobiochemistry, Semmelweis University, 1094 Budapest, HungaryBackground: Triethylene glycol dimethacrylate (TEGDMA) monomers released from resin matrix are toxic to dental pulp cells, induce apoptosis, oxidative stress and decrease viability. Recently, mitochondrial complex I (CI) was identified as a potential target of TEGDMA. In isolated mitochondria supported by CI, substrates oxidation and ATP synthesis were inhibited, reactive oxygen species production was stimulated. Contrary to that, respiratory Complex II was not impaired by TEGDMA. The beneficial effects of electron carrier compound methylene blue (MB) are proven in many disease models where mitochondrial involvement has been detected. In the present study, the bioenergetic effects of MB on TEGDMA-treated isolated mitochondria and on human dental pulp stem cells (DPSC) were analyzed. Methods: Isolated mitochondria and DPSC were acutely exposed to low millimolar concentrations of TEGDMA and 2 μM concentration of MB. Mitochondrial and cellular respiration and glycolytic flux were measured by high resolution respirometry and by Seahorse XF extracellular analyzer. Mitochondrial membrane potential was measured fluorimetrically. Results: MB partially restored the mitochondrial oxidation, rescued membrane potential in isolated mitochondria and significantly increased the impaired cellular O<sub>2</sub> consumption in the presence of TEGDMA. Conclusion: MB is able to protect against TEGDMA-induced CI damage, and might provide protective effects in resin monomer exposed cells.https://www.mdpi.com/1996-1944/13/16/3472DPSCmethylene bluemitochondriaoxygen consumptionTEGDMA |
| spellingShingle | Krisztina Mikulás Timea Komlódi Anna Földes Gergely Sváb Gergő Horváth Ádám Miklós Nagy Attila Ambrus Szabolcs Gyulai-Gaál István Gera Péter Hermann Gábor Varga László Tretter Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue Materials DPSC methylene blue mitochondria oxygen consumption TEGDMA |
| title | Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue |
| title_full | Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue |
| title_fullStr | Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue |
| title_full_unstemmed | Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue |
| title_short | Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue |
| title_sort | bioenergetic impairment of triethylene glycol dimethacrylate tegdma treated dental pulp stem cells dpscs and isolated brain mitochondria are amended by redox compound methylene blue |
| topic | DPSC methylene blue mitochondria oxygen consumption TEGDMA |
| url | https://www.mdpi.com/1996-1944/13/16/3472 |
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