Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing
Background/Aims The microbiological surveillance of endoscopes and automated flexible endoscope reprocessing have been proven to be two of the most difficult and controversial areas of infection control in endoscopy. The purpose of this study was to standardize a sampling method for assessing the ef...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Gastrointestinal Endoscopy
2018-09-01
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| Series: | Clinical Endoscopy |
| Subjects: | |
| Online Access: | http://www.e-ce.org/upload/pdf/ce-2018-012.pdf |
| _version_ | 1797669252689297408 |
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| author | Savina Ditommaso Monica Giacomuzzi Elisa Ricciardi Carla Zotti |
| author_facet | Savina Ditommaso Monica Giacomuzzi Elisa Ricciardi Carla Zotti |
| author_sort | Savina Ditommaso |
| collection | DOAJ |
| description | Background/Aims The microbiological surveillance of endoscopes and automated flexible endoscope reprocessing have been proven to be two of the most difficult and controversial areas of infection control in endoscopy. The purpose of this study was to standardize a sampling method for assessing the effectiveness of standard reprocessing operating procedures for flexible fiberoptic laryngoscopes (FFLs). Methods First, the sampling devices were directly inoculated with Bacillus atrophaeus spores; second, tissue non tissue (TNT) wipes were tested on artificially contaminated surfaces and on FFLs. Results Comparison of the sponges, cellulose, and TNT wipes indicated that the TNT wipes were more effective in releasing spores (93%) than the sponges (49%) and cellulose wipes (52%). The developed protocol provides a high efficiency for both collection and extraction from the stainless steel surface (87% of the spores were removed and released) and from the FFL (85% of the spores were removed and released), with relatively low standard deviations for recovery efficiency, particularly for the analysis of the FFL. Conclusions TNT wipes are more efficient for sampling surface areas, thereby aiding in the accuracy and reproducibility of environmental surveillance. |
| first_indexed | 2024-03-11T20:41:30Z |
| format | Article |
| id | doaj.art-8242a85a73c84e9bb6ebebc0bb41479b |
| institution | Directory Open Access Journal |
| issn | 2234-2400 2234-2443 |
| language | English |
| last_indexed | 2024-03-11T20:41:30Z |
| publishDate | 2018-09-01 |
| publisher | Korean Society of Gastrointestinal Endoscopy |
| record_format | Article |
| series | Clinical Endoscopy |
| spelling | doaj.art-8242a85a73c84e9bb6ebebc0bb41479b2023-10-02T02:08:02ZengKorean Society of Gastrointestinal EndoscopyClinical Endoscopy2234-24002234-24432018-09-0151546346910.5946/ce.2018.0127131Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after ReprocessingSavina DitommasoMonica Giacomuzzi0Elisa Ricciardi1Carla Zotti2 Department of Public Health and Pediatrics, University of Turin, Turin, Italy Department of Public Health and Pediatrics, University of Turin, Turin, Italy Department of Public Health and Pediatrics, University of Turin, Turin, ItalyBackground/Aims The microbiological surveillance of endoscopes and automated flexible endoscope reprocessing have been proven to be two of the most difficult and controversial areas of infection control in endoscopy. The purpose of this study was to standardize a sampling method for assessing the effectiveness of standard reprocessing operating procedures for flexible fiberoptic laryngoscopes (FFLs). Methods First, the sampling devices were directly inoculated with Bacillus atrophaeus spores; second, tissue non tissue (TNT) wipes were tested on artificially contaminated surfaces and on FFLs. Results Comparison of the sponges, cellulose, and TNT wipes indicated that the TNT wipes were more effective in releasing spores (93%) than the sponges (49%) and cellulose wipes (52%). The developed protocol provides a high efficiency for both collection and extraction from the stainless steel surface (87% of the spores were removed and released) and from the FFL (85% of the spores were removed and released), with relatively low standard deviations for recovery efficiency, particularly for the analysis of the FFL. Conclusions TNT wipes are more efficient for sampling surface areas, thereby aiding in the accuracy and reproducibility of environmental surveillance.http://www.e-ce.org/upload/pdf/ce-2018-012.pdfFlexible fiberoptic laryngoscopeDisinfectionSamplingWipes |
| spellingShingle | Savina Ditommaso Monica Giacomuzzi Elisa Ricciardi Carla Zotti Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing Clinical Endoscopy Flexible fiberoptic laryngoscope Disinfection Sampling Wipes |
| title | Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing |
| title_full | Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing |
| title_fullStr | Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing |
| title_full_unstemmed | Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing |
| title_short | Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing |
| title_sort | experimental study to develop a method for improving sample collection to monitor laryngoscopes after reprocessing |
| topic | Flexible fiberoptic laryngoscope Disinfection Sampling Wipes |
| url | http://www.e-ce.org/upload/pdf/ce-2018-012.pdf |
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