ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA
The purpose of this work was to study the antimicrobial, antibiofilm-forming and antioxidant properties of alcohol extracts of Arnica montana L. and Achillea millefolium L. The plants for the analysis were gathered in the territory of Velyky Berezny region, Transcarpathia. Ethyl and methyl extracts...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-02-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/1_2020/kryvtsova_1_2020.pdf |
| _version_ | 1797709143929257984 |
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| author | M. Kryvtsova J. Koščová |
| author_facet | M. Kryvtsova J. Koščová |
| author_sort | M. Kryvtsova |
| collection | DOAJ |
| description | The purpose of this work was to study the antimicrobial, antibiofilm-forming and antioxidant properties of alcohol extracts of Arnica montana L. and Achillea millefolium L. The plants for the analysis were gathered in the territory of Velyky Berezny region, Transcarpathia. Ethyl and methyl extracts were produced from inflorescences of Arnica montana L. and Achillea millefolium L. For the purpose of analysis, we used Staphylococcus genus bacteria that had been isolated from the mouth cavities and pharynx of human patients suffering from inflammatory diseases, by plating into differentially diagnostic nutrient media with subsequent identification. All isolates were characterized to be biofilm-forming.
The subject of the study was extract’s antimicrobial activity evaluated by the diffusion-into-agar method in standard 96-well plates using the spectrophotometric method and by measuring of antioxidant activity (DPPH test).
t was established that Arnica montana L. extracts exerted a more pronounced antimicrobial activity upon the analysed isolates of Staphylococcus genus bacteria. It was furthermore shown that Arnica montana L. extracts displayed an antimicrobial effect even upon MRSA of S. aureus. Extracts of Arnica montana L. and Achillea millefolium L. were shown to possess anti-biofilm forming properties.
Ethyl and methyl extracts of Arnica montana L.and Achillea millefolium L. were shown to reveal significant antioxidant activity.
Thus, our results indicated a need in further research of possible application of Arnica montana L. and Achillea millefolium L. extracts as anti-staphylococcal agents, which could be employed for the treatment of inflammatory processes in mouth cavity and oropharynx. |
| first_indexed | 2024-03-12T06:32:08Z |
| format | Article |
| id | doaj.art-824733313194464686e81e1b4b40084b |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T06:32:08Z |
| publishDate | 2020-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-824733313194464686e81e1b4b40084b2023-09-03T01:33:36ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2020-02-01131303710.15407/biotech13.01.030ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIAM. Kryvtsova0J. Koščová1Uzhhorod National University, UkraineUniversity of Veterinary Medicine and Pharmacy in Košice, SlovakiaThe purpose of this work was to study the antimicrobial, antibiofilm-forming and antioxidant properties of alcohol extracts of Arnica montana L. and Achillea millefolium L. The plants for the analysis were gathered in the territory of Velyky Berezny region, Transcarpathia. Ethyl and methyl extracts were produced from inflorescences of Arnica montana L. and Achillea millefolium L. For the purpose of analysis, we used Staphylococcus genus bacteria that had been isolated from the mouth cavities and pharynx of human patients suffering from inflammatory diseases, by plating into differentially diagnostic nutrient media with subsequent identification. All isolates were characterized to be biofilm-forming. The subject of the study was extract’s antimicrobial activity evaluated by the diffusion-into-agar method in standard 96-well plates using the spectrophotometric method and by measuring of antioxidant activity (DPPH test). t was established that Arnica montana L. extracts exerted a more pronounced antimicrobial activity upon the analysed isolates of Staphylococcus genus bacteria. It was furthermore shown that Arnica montana L. extracts displayed an antimicrobial effect even upon MRSA of S. aureus. Extracts of Arnica montana L. and Achillea millefolium L. were shown to possess anti-biofilm forming properties. Ethyl and methyl extracts of Arnica montana L.and Achillea millefolium L. were shown to reveal significant antioxidant activity. Thus, our results indicated a need in further research of possible application of Arnica montana L. and Achillea millefolium L. extracts as anti-staphylococcal agents, which could be employed for the treatment of inflammatory processes in mouth cavity and oropharynx.http://biotechnology.kiev.ua/images/1_2020/kryvtsova_1_2020.pdfantimicrobial effectantibiofilm formationplant extracts. |
| spellingShingle | M. Kryvtsova J. Koščová ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA Biotechnologia Acta antimicrobial effect antibiofilm formation plant extracts. |
| title | ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA |
| title_full | ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA |
| title_fullStr | ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA |
| title_full_unstemmed | ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA |
| title_short | ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA |
| title_sort | antibiofilm forming and antimicrobial activity of extracts of arnica montana l achillea millefolium l on staphylococcus genus bacteria |
| topic | antimicrobial effect antibiofilm formation plant extracts. |
| url | http://biotechnology.kiev.ua/images/1_2020/kryvtsova_1_2020.pdf |
| work_keys_str_mv | AT mkryvtsova antibiofilmformingandantimicrobialactivityofextractsofarnicamontanalachilleamillefoliumlonstaphylococcusgenusbacteria AT jkoscova antibiofilmformingandantimicrobialactivityofextractsofarnicamontanalachilleamillefoliumlonstaphylococcusgenusbacteria |