Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
Phosphorus (P) is an essential macronutrient for plant growth and development. Among adaptive strategies of plants to P deficiency, increased anthocyanin accumulation is widely observed in plants, which is tightly regulated by a set of genes at transcription levels. However, it remains unclear wheth...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/22/12348 |
| Summary: | Phosphorus (P) is an essential macronutrient for plant growth and development. Among adaptive strategies of plants to P deficiency, increased anthocyanin accumulation is widely observed in plants, which is tightly regulated by a set of genes at transcription levels. However, it remains unclear whether other key regulators might control anthocyanin synthesis through protein modification under P-deficient conditions. In the study, phosphate (Pi) starvation led to anthocyanin accumulations in soybean (<i>Glycine max</i>) leaves, accompanied with increased transcripts of a group of genes involved in anthocyanin synthesis. Meanwhile, transcripts of <i>GmCSN5A</i>/<i>B</i>, two members of the <i>COP9 signalosome subunit 5</i> (<i>CSN5</i>) family, were up-regulated in both young and old soybean leaves by Pi starvation. Furthermore, overexpressing <i>GmCSN5A</i> and <i>GmCSN5B</i> in <i>Arabidopsis thaliana</i> significantly resulted in anthocyanin accumulations in shoots, accompanied with increased transcripts of gene functions in anthocyanin synthesis including <i>AtPAL</i>, <i>AtCHS</i>, <i>AtF3H</i>, <i>AtF3</i>′<i>H</i>, <i>AtDFR</i>, <i>AtANS</i>, and <i>AtUF3GT</i> only under P-deficient conditions. Taken together, these results strongly suggest that P deficiency leads to increased anthocyanin synthesis through enhancing expression levels of genes involved in anthocyanin synthesis, which could be regulated by GmCSN5A and GmCSN5B. |
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| ISSN: | 1661-6596 1422-0067 |