Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2
The goal of this study was to evaluate the performance of a commercial reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay (Detect COVID-19 Test) in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A total of 202 human respiratory and viral cult...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/11/9/2344 |
| _version_ | 1827727082673668096 |
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| author | Janet Hei Yin Law Wai Sing Chan Tsun Leung Chan Edmond Shiu Kwan Ma Bone Siu Fai Tang |
| author_facet | Janet Hei Yin Law Wai Sing Chan Tsun Leung Chan Edmond Shiu Kwan Ma Bone Siu Fai Tang |
| author_sort | Janet Hei Yin Law |
| collection | DOAJ |
| description | The goal of this study was to evaluate the performance of a commercial reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay (Detect COVID-19 Test) in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A total of 202 human respiratory and viral culture specimens were tested retrospectively. The performance of the Detect COVID-19 Test was comparable to that of commercial real-time polymerase chain reaction assays (sensitivity: 93.42%; specificity: 100%), and better than that of the rapid antigen test (sensitivity: 48.00%; specificity: 100%) for specimens with threshold cycle (Ct) values of less than 30. The Beta, Delta, and Omicron variants of concern were successfully detected. With their simplicity of use and good assay sensitivity, point-of-care RT-LAMP assays may be a viable option for SARS-CoV-2 testing at home, or in regions without sophisticated laboratory facilities. |
| first_indexed | 2024-03-10T23:01:31Z |
| format | Article |
| id | doaj.art-8248eebd18cd479c9b1994f1ed267bc0 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T23:01:31Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-8248eebd18cd479c9b1994f1ed267bc02023-11-19T09:39:59ZengMDPI AGBiomedicines2227-90592023-08-01119234410.3390/biomedicines11092344Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2Janet Hei Yin Law0Wai Sing Chan1Tsun Leung Chan2Edmond Shiu Kwan Ma3Bone Siu Fai Tang4Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong SAR, ChinaDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong SAR, ChinaDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong SAR, ChinaDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong SAR, ChinaDepartment of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong SAR, ChinaThe goal of this study was to evaluate the performance of a commercial reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay (Detect COVID-19 Test) in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A total of 202 human respiratory and viral culture specimens were tested retrospectively. The performance of the Detect COVID-19 Test was comparable to that of commercial real-time polymerase chain reaction assays (sensitivity: 93.42%; specificity: 100%), and better than that of the rapid antigen test (sensitivity: 48.00%; specificity: 100%) for specimens with threshold cycle (Ct) values of less than 30. The Beta, Delta, and Omicron variants of concern were successfully detected. With their simplicity of use and good assay sensitivity, point-of-care RT-LAMP assays may be a viable option for SARS-CoV-2 testing at home, or in regions without sophisticated laboratory facilities.https://www.mdpi.com/2227-9059/11/9/2344COVID-19Detect COVID-19 TestRT-LAMPPOCTSARS-CoV-2variants of concern |
| spellingShingle | Janet Hei Yin Law Wai Sing Chan Tsun Leung Chan Edmond Shiu Kwan Ma Bone Siu Fai Tang Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 Biomedicines COVID-19 Detect COVID-19 Test RT-LAMP POCT SARS-CoV-2 variants of concern |
| title | Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 |
| title_full | Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 |
| title_fullStr | Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 |
| title_full_unstemmed | Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 |
| title_short | Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 |
| title_sort | evaluation of a commercial point of care rt lamp assay for rapid detection of sars cov 2 |
| topic | COVID-19 Detect COVID-19 Test RT-LAMP POCT SARS-CoV-2 variants of concern |
| url | https://www.mdpi.com/2227-9059/11/9/2344 |
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