Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing
The effects of each subtype-selective peroxisome proliferator activated receptor (PPAR) agonist (α, β/δ, γ) on corneal epithelial wound healing were investigated using a rat corneal alkali burn model. After the alkali burn, each PPAR agonist or vehicle ophthalmic solution was instilled topically ont...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/14/2/88 |
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| author | Yutaro Tobita Takeshi Arima Yuji Nakano Masaaki Uchiyama Akira Shimizu Hiroshi Takahashi |
| author_facet | Yutaro Tobita Takeshi Arima Yuji Nakano Masaaki Uchiyama Akira Shimizu Hiroshi Takahashi |
| author_sort | Yutaro Tobita |
| collection | DOAJ |
| description | The effects of each subtype-selective peroxisome proliferator activated receptor (PPAR) agonist (α, β/δ, γ) on corneal epithelial wound healing were investigated using a rat corneal alkali burn model. After the alkali burn, each PPAR agonist or vehicle ophthalmic solution was instilled topically onto the rat’s cornea. Corneal epithelial healing processes were evaluated by fluorescein staining. Pathological analyses and real-time reverse transcription polymerase chain reactions were performed to evaluate Ki67 (proliferative maker) expression and inflammatory findings. The area of the corneal epithelial defect at 12 h and 24 h after the alkali burn was significantly smaller in each PPAR group than in the vehicle group. Ki67 mRNA expression was increased in the PPARβ/δ group, whereas mRNA expressions of inflammatory cytokines were suppressed in all of the PPAR agonist groups. Nuclear factor kappa B (NF-κB) was the most suppressed in the PPARγ group. The accelerated corneal epithelial healing effects of each PPAR ligand were thought to be related to the promotion of proliferative capacity and inhibition of inflammation. |
| first_indexed | 2024-03-09T03:41:32Z |
| format | Article |
| id | doaj.art-824a915e541f42f38076fac4fec61735 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T03:41:32Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-824a915e541f42f38076fac4fec617352023-12-03T14:40:21ZengMDPI AGPharmaceuticals1424-82472021-01-011428810.3390/ph14020088Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound HealingYutaro Tobita0Takeshi Arima1Yuji Nakano2Masaaki Uchiyama3Akira Shimizu4Hiroshi Takahashi5Department of Ophthalmology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, JapanDepartment of Ophthalmology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, JapanDepartment of Ophthalmology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, JapanDepartment of Ophthalmology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, JapanDepartment of Analytic Human Pathology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, JapanDepartment of Ophthalmology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, JapanThe effects of each subtype-selective peroxisome proliferator activated receptor (PPAR) agonist (α, β/δ, γ) on corneal epithelial wound healing were investigated using a rat corneal alkali burn model. After the alkali burn, each PPAR agonist or vehicle ophthalmic solution was instilled topically onto the rat’s cornea. Corneal epithelial healing processes were evaluated by fluorescein staining. Pathological analyses and real-time reverse transcription polymerase chain reactions were performed to evaluate Ki67 (proliferative maker) expression and inflammatory findings. The area of the corneal epithelial defect at 12 h and 24 h after the alkali burn was significantly smaller in each PPAR group than in the vehicle group. Ki67 mRNA expression was increased in the PPARβ/δ group, whereas mRNA expressions of inflammatory cytokines were suppressed in all of the PPAR agonist groups. Nuclear factor kappa B (NF-κB) was the most suppressed in the PPARγ group. The accelerated corneal epithelial healing effects of each PPAR ligand were thought to be related to the promotion of proliferative capacity and inhibition of inflammation.https://www.mdpi.com/1424-8247/14/2/88corneal epithelial wound healingPPARalkali burn |
| spellingShingle | Yutaro Tobita Takeshi Arima Yuji Nakano Masaaki Uchiyama Akira Shimizu Hiroshi Takahashi Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing Pharmaceuticals corneal epithelial wound healing PPAR alkali burn |
| title | Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing |
| title_full | Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing |
| title_fullStr | Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing |
| title_full_unstemmed | Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing |
| title_short | Effects of Selective Peroxisome Proliferator Activated Receptor Agonists on Corneal Epithelial Wound Healing |
| title_sort | effects of selective peroxisome proliferator activated receptor agonists on corneal epithelial wound healing |
| topic | corneal epithelial wound healing PPAR alkali burn |
| url | https://www.mdpi.com/1424-8247/14/2/88 |
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