Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure
Bisbenzimidazoles have a broad spectrum of potential applications: radioprotectors, drug delivery vectors, antiviral agents, etc. At the same time, they seem to be promising fluorescent probes for radiation measurements. Therefore, in the present work, a fluorescent response to X-ray irradiation of...
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MDPI AG
2023-06-01
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| author | Maria A. Kolyvanova Mikhail A. Klimovich Ekaterina D. Koshevaya Evgeny A. Nikitin Nikita S. Lifanovsky Vladimir Y. Tyurin Alexandr V. Belousov Aleksei V. Trofimov Vladimir A. Kuzmin Vladimir N. Morozov |
| author_facet | Maria A. Kolyvanova Mikhail A. Klimovich Ekaterina D. Koshevaya Evgeny A. Nikitin Nikita S. Lifanovsky Vladimir Y. Tyurin Alexandr V. Belousov Aleksei V. Trofimov Vladimir A. Kuzmin Vladimir N. Morozov |
| author_sort | Maria A. Kolyvanova |
| collection | DOAJ |
| description | Bisbenzimidazoles have a broad spectrum of potential applications: radioprotectors, drug delivery vectors, antiviral agents, etc. At the same time, they seem to be promising fluorescent probes for radiation measurements. Therefore, in the present work, a fluorescent response to X-ray irradiation of Hoechst 33258, one of the most widely known representatives of the bisbenzimidazole family, was studied for the first time. Irradiation of the dye was performed in aqueous and organic solutions (DMSO and glycerol), as well as in their mixtures. It is shown that the reaction of the dye to radiation exposure is very versatile and may be controlled by the solvent properties, which makes it possible to build relationships between the absorbed dose and a wide variety of parameters of its fluorescence signal. For example, irradiation may induce fluorescence quenching caused by the degradation of the dye, a change in the position of the fluorescence band maximum due to the modification of the dye molecules or to the radiation-induced changes in the properties of the medium, as well as a fluorescence flare-up mediated by the changes in pH. |
| first_indexed | 2024-03-11T02:02:07Z |
| format | Article |
| id | doaj.art-824f832691e54e4fa274ef1c7b4fd71d |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-11T02:02:07Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-824f832691e54e4fa274ef1c7b4fd71d2023-11-18T12:08:06ZengMDPI AGPhotonics2304-67322023-06-0110667110.3390/photonics10060671Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation ExposureMaria A. Kolyvanova0Mikhail A. Klimovich1Ekaterina D. Koshevaya2Evgeny A. Nikitin3Nikita S. Lifanovsky4Vladimir Y. Tyurin5Alexandr V. Belousov6Aleksei V. Trofimov7Vladimir A. Kuzmin8Vladimir N. Morozov9Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina, 119334 Moscow, RussiaBurnazyan Federal Medical Biophysical Center, Federal Medical Biological Agency of the Russian Federation, 23 Marshala Novikova, 123182 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 1–3 Leninskie Gory, 119991 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina, 119334 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 1–3 Leninskie Gory, 119991 Moscow, RussiaBurnazyan Federal Medical Biophysical Center, Federal Medical Biological Agency of the Russian Federation, 23 Marshala Novikova, 123182 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina, 119334 Moscow, RussiaBisbenzimidazoles have a broad spectrum of potential applications: radioprotectors, drug delivery vectors, antiviral agents, etc. At the same time, they seem to be promising fluorescent probes for radiation measurements. Therefore, in the present work, a fluorescent response to X-ray irradiation of Hoechst 33258, one of the most widely known representatives of the bisbenzimidazole family, was studied for the first time. Irradiation of the dye was performed in aqueous and organic solutions (DMSO and glycerol), as well as in their mixtures. It is shown that the reaction of the dye to radiation exposure is very versatile and may be controlled by the solvent properties, which makes it possible to build relationships between the absorbed dose and a wide variety of parameters of its fluorescence signal. For example, irradiation may induce fluorescence quenching caused by the degradation of the dye, a change in the position of the fluorescence band maximum due to the modification of the dye molecules or to the radiation-induced changes in the properties of the medium, as well as a fluorescence flare-up mediated by the changes in pH.https://www.mdpi.com/2304-6732/10/6/671Hoechst 33258bisbenzimidazoleschemical dosimetryradiation measurementsfluorescenceionizing radiation |
| spellingShingle | Maria A. Kolyvanova Mikhail A. Klimovich Ekaterina D. Koshevaya Evgeny A. Nikitin Nikita S. Lifanovsky Vladimir Y. Tyurin Alexandr V. Belousov Aleksei V. Trofimov Vladimir A. Kuzmin Vladimir N. Morozov Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure Photonics Hoechst 33258 bisbenzimidazoles chemical dosimetry radiation measurements fluorescence ionizing radiation |
| title | Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure |
| title_full | Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure |
| title_fullStr | Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure |
| title_full_unstemmed | Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure |
| title_short | Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure |
| title_sort | chemical dosimetry using bisbenzimidazoles solvent dependent fluorescence response of hoechst 33258 to radiation exposure |
| topic | Hoechst 33258 bisbenzimidazoles chemical dosimetry radiation measurements fluorescence ionizing radiation |
| url | https://www.mdpi.com/2304-6732/10/6/671 |
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