Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins
<p>Abstract</p> <p>Background</p> <p>In order to establish a successful infection in the human host, the malaria parasite <it>Plasmodium falciparum </it>must establish interactions with a variety of human proteins on the surface of different cell types, as w...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-10-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/7/1/211 |
| _version_ | 1811260168516665344 |
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| author | Sahasrabudhe Sudhir Bell Russell Chettier Rakesh LaCount Douglas J McKinlay Anastasia Vignali Marissa Hughes Robert E Fields Stanley |
| author_facet | Sahasrabudhe Sudhir Bell Russell Chettier Rakesh LaCount Douglas J McKinlay Anastasia Vignali Marissa Hughes Robert E Fields Stanley |
| author_sort | Sahasrabudhe Sudhir |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In order to establish a successful infection in the human host, the malaria parasite <it>Plasmodium falciparum </it>must establish interactions with a variety of human proteins on the surface of different cell types, as well as with proteins inside the host cells. To better understand this aspect of malaria pathogenesis, a study was conducted with the goal of identifying interactions between proteins of the parasite and those of its human host.</p> <p>Methods</p> <p>A modified yeast two-hybrid methodology that preferentially selects protein fragments that can be expressed in yeast was used to conduct high-throughput screens with <it>P. falciparum </it>protein fragments against human liver and cerebellum libraries. The resulting dataset was analyzed to exclude interactions that are not likely to occur in the human host during infection.</p> <p>Results</p> <p>An initial set of 2,200 interactions was curated to remove proteins that are unlikely to play a role in pathogenesis based on their annotation or localization, and proteins that behave promiscuously in the two-hybrid assay, resulting in a final dataset of 456 interactions. A cluster that implicates binding between <it>P. falciparum </it>PFE1590w/ETRAMP5, a putative parasitophorous vacuole membrane protein, and human apolipoproteins ApoA, ApoB and ApoE was selected for further analysis. Different isoforms of ApoE, which are associated with different outcomes of malaria infection, were shown to display differential interactions with PFE1590w.</p> <p>Conclusion</p> <p>A dataset of interactions between proteins of <it>P. falciparum </it>and those of its human host was generated. The preferential interaction of the <it>P. falciparum </it>PFE1590w protein with the human ApoE ε3 and ApoE ε4 isoforms, but not the ApoE ε2 isoform, supports the hypothesis that ApoE genotype affects risk of malaria infection. The dataset contains other interactions of potential relevance to disease that may identify possible vaccine candidates and drug targets.</p> |
| first_indexed | 2024-04-12T18:43:31Z |
| format | Article |
| id | doaj.art-82507b5741a443b5a0c025b212605812 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-12T18:43:31Z |
| publishDate | 2008-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-82507b5741a443b5a0c025b2126058122022-12-22T03:20:42ZengBMCMalaria Journal1475-28752008-10-017121110.1186/1475-2875-7-211Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteinsSahasrabudhe SudhirBell RussellChettier RakeshLaCount Douglas JMcKinlay AnastasiaVignali MarissaHughes Robert EFields Stanley<p>Abstract</p> <p>Background</p> <p>In order to establish a successful infection in the human host, the malaria parasite <it>Plasmodium falciparum </it>must establish interactions with a variety of human proteins on the surface of different cell types, as well as with proteins inside the host cells. To better understand this aspect of malaria pathogenesis, a study was conducted with the goal of identifying interactions between proteins of the parasite and those of its human host.</p> <p>Methods</p> <p>A modified yeast two-hybrid methodology that preferentially selects protein fragments that can be expressed in yeast was used to conduct high-throughput screens with <it>P. falciparum </it>protein fragments against human liver and cerebellum libraries. The resulting dataset was analyzed to exclude interactions that are not likely to occur in the human host during infection.</p> <p>Results</p> <p>An initial set of 2,200 interactions was curated to remove proteins that are unlikely to play a role in pathogenesis based on their annotation or localization, and proteins that behave promiscuously in the two-hybrid assay, resulting in a final dataset of 456 interactions. A cluster that implicates binding between <it>P. falciparum </it>PFE1590w/ETRAMP5, a putative parasitophorous vacuole membrane protein, and human apolipoproteins ApoA, ApoB and ApoE was selected for further analysis. Different isoforms of ApoE, which are associated with different outcomes of malaria infection, were shown to display differential interactions with PFE1590w.</p> <p>Conclusion</p> <p>A dataset of interactions between proteins of <it>P. falciparum </it>and those of its human host was generated. The preferential interaction of the <it>P. falciparum </it>PFE1590w protein with the human ApoE ε3 and ApoE ε4 isoforms, but not the ApoE ε2 isoform, supports the hypothesis that ApoE genotype affects risk of malaria infection. The dataset contains other interactions of potential relevance to disease that may identify possible vaccine candidates and drug targets.</p>http://www.malariajournal.com/content/7/1/211 |
| spellingShingle | Sahasrabudhe Sudhir Bell Russell Chettier Rakesh LaCount Douglas J McKinlay Anastasia Vignali Marissa Hughes Robert E Fields Stanley Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins Malaria Journal |
| title | Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins |
| title_full | Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins |
| title_fullStr | Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins |
| title_full_unstemmed | Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins |
| title_short | Interaction of an atypical <it>Plasmodium falciparum </it>ETRAMP with human apolipoproteins |
| title_sort | interaction of an atypical it plasmodium falciparum it etramp with human apolipoproteins |
| url | http://www.malariajournal.com/content/7/1/211 |
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