Urinary microbiome profile in men with genitourinary malignancies
Purpose: Recent advances in molecular biology technology have allowed identification of microbial communities in the urinary tract, and urinary microbiome is associated with various urological diseases. In this study, we aimed to characterize the urinary microbiome of genitourinary malignancies. Ma...
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| Format: | Article |
| Language: | English |
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Korean Urological Association
2022-09-01
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| Series: | Investigative and Clinical Urology |
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| Online Access: | https://www.icurology.org/pdf/10.4111/icu.20220124 |
| _version_ | 1828409824499466240 |
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| author | Hyun Kyu Ahn Kwangmin Kim Junhyung Park Kwang Hyun Kim |
| author_facet | Hyun Kyu Ahn Kwangmin Kim Junhyung Park Kwang Hyun Kim |
| author_sort | Hyun Kyu Ahn |
| collection | DOAJ |
| description | Purpose: Recent advances in molecular biology technology have allowed identification of microbial communities in the urinary tract, and urinary microbiome is associated with various urological diseases. In this study, we aimed to characterize the urinary microbiome of genitourinary malignancies.
Materials and Methods: Metagenomic analysis of urinary DNA was performed in 85 patients including 30 with bladder cancer (BC), 27 with prostate cancer (PC), 12 with renal cancer (RC), and 16 with non-cancer (NC). 16S rRNA gene sequencing was conducted after amplification of the V3–V4 region.
Results: PC and RC had significantly lower Shannon index than BC, and beta diversity showed significantly different microbiome composition between four groups. We identified six genera of Cutibacterium, Peptoniphilus, Sphingomonas, Staphylococcus, Micrococcus, and Moraxella, which showed significantly different abundance between the four groups. When each of the malignancies were compared to NC at the species level, Micrococcus sp. was significantly increased in BC. We also identified 12 and five species with increased populations in PC and RC, respectively. Of these, Cutibacterium acnes, Cutibacterium granulosum, Peptoniphilus lacydonensis, and Tessaracoccus were significantly increased in both PC and RC.
Conclusions: Urinary microbiome composition was different depending on the type of genitourinary malignancies, and we identified bacteria that are significantly associated with each type of malignancy. Specifically, several bacterial species were associated both PC and RC, suggesting that PC and RC share a similar pathogenesis-related urinary microbiome. |
| first_indexed | 2024-12-10T12:01:47Z |
| format | Article |
| id | doaj.art-8250888a819244649299f0c67bc03443 |
| institution | Directory Open Access Journal |
| issn | 2466-0493 2466-054X |
| language | English |
| last_indexed | 2024-12-10T12:01:47Z |
| publishDate | 2022-09-01 |
| publisher | Korean Urological Association |
| record_format | Article |
| series | Investigative and Clinical Urology |
| spelling | doaj.art-8250888a819244649299f0c67bc034432022-12-22T01:49:36ZengKorean Urological AssociationInvestigative and Clinical Urology2466-04932466-054X2022-09-0163556957610.4111/icu.20220124Urinary microbiome profile in men with genitourinary malignanciesHyun Kyu Ahn0https://orcid.org/0000-0002-1996-6249Kwangmin Kim1https://orcid.org/0000-0003-3306-1021Junhyung Park2https://orcid.org/0000-0002-0805-6362Kwang Hyun Kim3https://orcid.org/0000-0002-6264-5109Department of Urology, Ewha Womans University Seoul Hospital, Seoul, Korea.3BIGS CO., LTD., Hwaseong, Korea.3BIGS CO., LTD., Hwaseong, Korea.Department of Urology, Ewha Womans University Seoul Hospital, Seoul, Korea.Purpose: Recent advances in molecular biology technology have allowed identification of microbial communities in the urinary tract, and urinary microbiome is associated with various urological diseases. In this study, we aimed to characterize the urinary microbiome of genitourinary malignancies. Materials and Methods: Metagenomic analysis of urinary DNA was performed in 85 patients including 30 with bladder cancer (BC), 27 with prostate cancer (PC), 12 with renal cancer (RC), and 16 with non-cancer (NC). 16S rRNA gene sequencing was conducted after amplification of the V3–V4 region. Results: PC and RC had significantly lower Shannon index than BC, and beta diversity showed significantly different microbiome composition between four groups. We identified six genera of Cutibacterium, Peptoniphilus, Sphingomonas, Staphylococcus, Micrococcus, and Moraxella, which showed significantly different abundance between the four groups. When each of the malignancies were compared to NC at the species level, Micrococcus sp. was significantly increased in BC. We also identified 12 and five species with increased populations in PC and RC, respectively. Of these, Cutibacterium acnes, Cutibacterium granulosum, Peptoniphilus lacydonensis, and Tessaracoccus were significantly increased in both PC and RC. Conclusions: Urinary microbiome composition was different depending on the type of genitourinary malignancies, and we identified bacteria that are significantly associated with each type of malignancy. Specifically, several bacterial species were associated both PC and RC, suggesting that PC and RC share a similar pathogenesis-related urinary microbiome.https://www.icurology.org/pdf/10.4111/icu.20220124metagenomemicrobiotaurineurologic neoplasms |
| spellingShingle | Hyun Kyu Ahn Kwangmin Kim Junhyung Park Kwang Hyun Kim Urinary microbiome profile in men with genitourinary malignancies Investigative and Clinical Urology metagenome microbiota urine urologic neoplasms |
| title | Urinary microbiome profile in men with genitourinary malignancies |
| title_full | Urinary microbiome profile in men with genitourinary malignancies |
| title_fullStr | Urinary microbiome profile in men with genitourinary malignancies |
| title_full_unstemmed | Urinary microbiome profile in men with genitourinary malignancies |
| title_short | Urinary microbiome profile in men with genitourinary malignancies |
| title_sort | urinary microbiome profile in men with genitourinary malignancies |
| topic | metagenome microbiota urine urologic neoplasms |
| url | https://www.icurology.org/pdf/10.4111/icu.20220124 |
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