МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС
<p>Soil samples were collected from Khentii and Tuv provinces in Mongolia. Two strains (N11, N22) were selected for polyphasic approach which including morphological, physiological and phylogenetic analysis. <br />Phylogenetic analysis revealed that the strain N22 has highest the 16S rRN...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Mongolian Academy of Sciences
2016-11-01
|
Series: | Proceedings of the Mongolian Academy of Sciences |
Subjects: | |
Online Access: | https://www.mongoliajol.info/index.php/PMAS/article/view/692 |
_version_ | 1818290572766478336 |
---|---|
author | Б Пагмадулам Мониша Канна Д Цэрэндулам Ц Рэнцэнханд |
author_facet | Б Пагмадулам Мониша Канна Д Цэрэндулам Ц Рэнцэнханд |
author_sort | Б Пагмадулам |
collection | DOAJ |
description | <p>Soil samples were collected from Khentii and Tuv provinces in Mongolia. Two strains (N11, N22) were selected for polyphasic approach which including morphological, physiological and phylogenetic analysis. <br />Phylogenetic analysis revealed that the strain N22 has highest the 16S rRNA similarity of 99.39% with Streptomyces ghanaensis NBRC 15414(T). The 16S rRNA genes sequence of 1418 nucleotides was generated for N11 and compared to the validly described species of genus Streptomyces as closest neighbors. Phylogenetic analysis revealed that the strain N11 has highest the 16S rRNA similarity of 98.51% with Streptomyces yerevanensis NBRC 12517(T). Also some phenotypic characteristics were different from type strains. Preliminary study shows that strain N11 might be new actinomycete species. However, need to determine the genetic distance between two microorganisms by using DNA-DNA hybridization methods.</p> |
first_indexed | 2024-12-13T02:30:18Z |
format | Article |
id | doaj.art-82540cdda09644f19a67bfb6bff5d802 |
institution | Directory Open Access Journal |
issn | 2310-4716 2312-2994 |
language | English |
last_indexed | 2024-12-13T02:30:18Z |
publishDate | 2016-11-01 |
publisher | Mongolian Academy of Sciences |
record_format | Article |
series | Proceedings of the Mongolian Academy of Sciences |
spelling | doaj.art-82540cdda09644f19a67bfb6bff5d8022022-12-22T00:02:31ZengMongolian Academy of SciencesProceedings of the Mongolian Academy of Sciences2310-47162312-29942016-11-01563667210.5564/pmas.v56i3.692645МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭСБ Пагмадулам0Мониша Канна1Д Цэрэндулам2Ц Рэнцэнханд3Ерөнхий болон сорилын биологийн хүрээлэнгийн Микробын Нийлэгжлийн Лаборатори, Шинжлэх ухааны АкадемиМикробын технологийн лаборатори, АНДК, Дэлигийн Их СургуульЕрөнхий болон сорилын биологийн хүрээлэнгийн Микробын Нийлэгжлийн Лаборатори, Шинжлэх ухааны АкадемиЕрөнхий болон сорилын биологийн хүрээлэнгийн Микробын Нийлэгжлийн Лаборатори, Шинжлэх ухааны Академи<p>Soil samples were collected from Khentii and Tuv provinces in Mongolia. Two strains (N11, N22) were selected for polyphasic approach which including morphological, physiological and phylogenetic analysis. <br />Phylogenetic analysis revealed that the strain N22 has highest the 16S rRNA similarity of 99.39% with Streptomyces ghanaensis NBRC 15414(T). The 16S rRNA genes sequence of 1418 nucleotides was generated for N11 and compared to the validly described species of genus Streptomyces as closest neighbors. Phylogenetic analysis revealed that the strain N11 has highest the 16S rRNA similarity of 98.51% with Streptomyces yerevanensis NBRC 12517(T). Also some phenotypic characteristics were different from type strains. Preliminary study shows that strain N11 might be new actinomycete species. However, need to determine the genetic distance between two microorganisms by using DNA-DNA hybridization methods.</p>https://www.mongoliajol.info/index.php/PMAS/article/view/692Актиномицет, 16S рРНХ ген, ангилалзүй, филогенетик |
spellingShingle | Б Пагмадулам Мониша Канна Д Цэрэндулам Ц Рэнцэнханд МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС Proceedings of the Mongolian Academy of Sciences Актиномицет, 16S рРНХ ген, ангилалзүй, филогенетик |
title | МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС |
title_full | МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС |
title_fullStr | МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС |
title_full_unstemmed | МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС |
title_short | МОНГОЛ ОРНЫ ХӨРСНӨӨС ЯЛГАСАН АКТИНОМИЦЕТИЙН АНГИЛАЛЗҮЙН СУДАЛГААНЫ ДҮНГЭЭС |
title_sort | монгол орны хөрснөөс ялгасан актиномицетийн ангилалзүйн судалгааны дүнгээс |
topic | Актиномицет, 16S рРНХ ген, ангилалзүй, филогенетик |
url | https://www.mongoliajol.info/index.php/PMAS/article/view/692 |
work_keys_str_mv | AT bpagmadulam mongolornyhөrsnөөsâlgasanaktinomicetijnangilalzүjnsudalgaanydүngéés AT monišakanna mongolornyhөrsnөөsâlgasanaktinomicetijnangilalzүjnsudalgaanydүngéés AT dcéréndulam mongolornyhөrsnөөsâlgasanaktinomicetijnangilalzүjnsudalgaanydүngéés AT créncénhand mongolornyhөrsnөөsâlgasanaktinomicetijnangilalzүjnsudalgaanydүngéés |