A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice
Abstract Background Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHLP gene, is responsible for phytyl biosynthesis. The light-harvesting like protein LIL3 was suggested to be required for stability of GGR and pro...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-2028-z |
| _version_ | 1818150869648015360 |
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| author | Chunmei Li Xin Liu Jihong Pan Jia Guo Qian Wang Congping Chen Na Li Kuan Zhang Bin Yang Changhui Sun Xiaojian Deng Pingrong Wang |
| author_facet | Chunmei Li Xin Liu Jihong Pan Jia Guo Qian Wang Congping Chen Na Li Kuan Zhang Bin Yang Changhui Sun Xiaojian Deng Pingrong Wang |
| author_sort | Chunmei Li |
| collection | DOAJ |
| description | Abstract Background Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHLP gene, is responsible for phytyl biosynthesis. The light-harvesting like protein LIL3 was suggested to be required for stability of GGR and protochlorophyllide oxidoreductase in Arabidopsis. Results In this study, we isolated a yellow-green leaf mutant, 637ys, in rice (Oryza sativa). The mutant accumulated majority of Chls with unsaturated geranylgeraniol side chains and displayed a yellow-green leaf phenotype through the whole growth period. The development of chloroplasts was suppressed, and the major agronomic traits, especially No. of productive panicles per plant and of spikelets per panicle, dramatically decreased in 637ys. Besides, the mutant exhibited to be sensitive to light intensity and deficiency of tocopherols without obvious alteration in tocotrienols in leaves and grains. Map-based cloning and complementation experiment demonstrated that a point mutation on the OsLIL3 gene accounted for the mutant phenotype of 637ys. OsLIL3 is mainly expressed in green tissues, and its encoded protein is targeted to the chloroplast. Furthermore, the 637ys 502ys (lil3 chlp) double mutant exclusively accumulated geranylgeranyl Chl and exhibited lethality at the three-leaf stage. Conclusions We identified the OsLIL3 gene through a map-based cloning approach. Meanwhile, we demonstrated that OsLIL3 is of extreme importance to the function of OsGGR, and that the complete replacement of phytyl side chain of chlorophyll by geranylgeranyl chain could be fatal to plant survival in rice. |
| first_indexed | 2024-12-11T13:29:47Z |
| format | Article |
| id | doaj.art-8254190da06945c886aea25553c2f0d0 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T13:29:47Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-8254190da06945c886aea25553c2f0d02022-12-22T01:05:19ZengBMCBMC Plant Biology1471-22292019-10-0119111510.1186/s12870-019-2028-zA lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in riceChunmei Li0Xin Liu1Jihong Pan2Jia Guo3Qian Wang4Congping Chen5Na Li6Kuan Zhang7Bin Yang8Changhui Sun9Xiaojian Deng10Pingrong Wang11Rice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityRice Research Institute, Sichuan Agricultural UniversityAbstract Background Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHLP gene, is responsible for phytyl biosynthesis. The light-harvesting like protein LIL3 was suggested to be required for stability of GGR and protochlorophyllide oxidoreductase in Arabidopsis. Results In this study, we isolated a yellow-green leaf mutant, 637ys, in rice (Oryza sativa). The mutant accumulated majority of Chls with unsaturated geranylgeraniol side chains and displayed a yellow-green leaf phenotype through the whole growth period. The development of chloroplasts was suppressed, and the major agronomic traits, especially No. of productive panicles per plant and of spikelets per panicle, dramatically decreased in 637ys. Besides, the mutant exhibited to be sensitive to light intensity and deficiency of tocopherols without obvious alteration in tocotrienols in leaves and grains. Map-based cloning and complementation experiment demonstrated that a point mutation on the OsLIL3 gene accounted for the mutant phenotype of 637ys. OsLIL3 is mainly expressed in green tissues, and its encoded protein is targeted to the chloroplast. Furthermore, the 637ys 502ys (lil3 chlp) double mutant exclusively accumulated geranylgeranyl Chl and exhibited lethality at the three-leaf stage. Conclusions We identified the OsLIL3 gene through a map-based cloning approach. Meanwhile, we demonstrated that OsLIL3 is of extreme importance to the function of OsGGR, and that the complete replacement of phytyl side chain of chlorophyll by geranylgeranyl chain could be fatal to plant survival in rice.http://link.springer.com/article/10.1186/s12870-019-2028-zChlorophyll biosynthesisGeranylgeranyl reductaseLeaf-color mutantLight-harvesting like proteinLIL3 genePhytyl residue |
| spellingShingle | Chunmei Li Xin Liu Jihong Pan Jia Guo Qian Wang Congping Chen Na Li Kuan Zhang Bin Yang Changhui Sun Xiaojian Deng Pingrong Wang A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice BMC Plant Biology Chlorophyll biosynthesis Geranylgeranyl reductase Leaf-color mutant Light-harvesting like protein LIL3 gene Phytyl residue |
| title | A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice |
| title_full | A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice |
| title_fullStr | A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice |
| title_full_unstemmed | A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice |
| title_short | A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice |
| title_sort | lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice |
| topic | Chlorophyll biosynthesis Geranylgeranyl reductase Leaf-color mutant Light-harvesting like protein LIL3 gene Phytyl residue |
| url | http://link.springer.com/article/10.1186/s12870-019-2028-z |
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