Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study
Ksenia Loskutova,1 Mar Torras,1 Ying Zhao,2 Anna J Svagan,3 Dmitry Grishenkov1 1Department of Biomedical Engineering and Health Systems, KTH Royal Institute of Technology, Huddinge, SE-141 57, Sweden; 2Department of Laboratory Medicine, Karolinska Institute, Huddinge, SE-141 57, Sweden; 3Department...
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| Format: | Article |
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Dove Medical Press
2023-04-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/cellulose-nanofiber-coated-perfluoropentane-droplets-fabrication-and-b-peer-reviewed-fulltext-article-IJN |
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| author | Loskutova K Torras M Zhao Y Svagan AJ Grishenkov D |
| author_facet | Loskutova K Torras M Zhao Y Svagan AJ Grishenkov D |
| author_sort | Loskutova K |
| collection | DOAJ |
| description | Ksenia Loskutova,1 Mar Torras,1 Ying Zhao,2 Anna J Svagan,3 Dmitry Grishenkov1 1Department of Biomedical Engineering and Health Systems, KTH Royal Institute of Technology, Huddinge, SE-141 57, Sweden; 2Department of Laboratory Medicine, Karolinska Institute, Huddinge, SE-141 57, Sweden; 3Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, SE-100 44, SwedenCorrespondence: Ksenia Loskutova, Department of Biomedical Engineering and Health Systems, KTH Royal Institute of Technology, Hälsovägen 11C, Huddinge, SE-14157, Sweden, Tel +46 707 26 76 77, Email ksenial@kth.sePurpose: To study the effect of cellulose nanofiber (CNF)-shelled perfluoropentane (PFP) droplets on the cell viability of 4T1 breast cancer cells with or without the addition of non-encapsulated paclitaxel.Methods: The CNF-shelled PFP droplets were produced by mixing a CNF suspension and PFP using a homogenizer. The volume size distribution and concentration of CNF-shelled PFP droplets were estimated from images taken with an optical microscope and analyzed using Fiji software and an in-house Matlab script. The thermal stability was qualitatively assessed by comparing the size distribution and concentration of CNF-shelled PFP droplets at room temperature (~22°) and 37°C. The cell viability of 4T1 cells was measured using a 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, a hemolysis assay was performed to assess blood compatibility of CNF-shelled PFP droplets.Results: The droplet diameter and concentration of CNF-shelled PFP droplets decreased after 48 hours at both room temperature and 37°C. In addition, the decrease in concentration was more significant at 37°C, from 3.50 ± 0.64× 106 droplets/mL to 1.94 ± 0.10× 106 droplets/mL, than at room temperature, from 3.65 ± 0.29× 106 droplets/mL to 2.56 ± 0.22× 106 droplets/mL. The 4T1 cell viability decreased with increased exposure time and concentration of paclitaxel, but it was not affected by the presence of CNF-shelled PFP droplets. No hemolysis was observed at any concentration of CNF-shelled PFP droplets.Conclusion: CNF-shelled PFP droplets have the potential to be applied as drug carriers in ultrasound-mediated therapy.Keywords: cell viability, ultrasound-mediated therapy, paclitaxel, cellulose nanofibers, biocompatibility, 4T1, Pickering emulsion |
| first_indexed | 2024-04-09T18:28:49Z |
| format | Article |
| id | doaj.art-8254eae4b2ed42999128ce85918c6638 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-04-09T18:28:49Z |
| publishDate | 2023-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-8254eae4b2ed42999128ce85918c66382023-04-11T19:04:59ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-04-01Volume 181835184782827Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility StudyLoskutova KTorras MZhao YSvagan AJGrishenkov DKsenia Loskutova,1 Mar Torras,1 Ying Zhao,2 Anna J Svagan,3 Dmitry Grishenkov1 1Department of Biomedical Engineering and Health Systems, KTH Royal Institute of Technology, Huddinge, SE-141 57, Sweden; 2Department of Laboratory Medicine, Karolinska Institute, Huddinge, SE-141 57, Sweden; 3Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, SE-100 44, SwedenCorrespondence: Ksenia Loskutova, Department of Biomedical Engineering and Health Systems, KTH Royal Institute of Technology, Hälsovägen 11C, Huddinge, SE-14157, Sweden, Tel +46 707 26 76 77, Email ksenial@kth.sePurpose: To study the effect of cellulose nanofiber (CNF)-shelled perfluoropentane (PFP) droplets on the cell viability of 4T1 breast cancer cells with or without the addition of non-encapsulated paclitaxel.Methods: The CNF-shelled PFP droplets were produced by mixing a CNF suspension and PFP using a homogenizer. The volume size distribution and concentration of CNF-shelled PFP droplets were estimated from images taken with an optical microscope and analyzed using Fiji software and an in-house Matlab script. The thermal stability was qualitatively assessed by comparing the size distribution and concentration of CNF-shelled PFP droplets at room temperature (~22°) and 37°C. The cell viability of 4T1 cells was measured using a 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, a hemolysis assay was performed to assess blood compatibility of CNF-shelled PFP droplets.Results: The droplet diameter and concentration of CNF-shelled PFP droplets decreased after 48 hours at both room temperature and 37°C. In addition, the decrease in concentration was more significant at 37°C, from 3.50 ± 0.64× 106 droplets/mL to 1.94 ± 0.10× 106 droplets/mL, than at room temperature, from 3.65 ± 0.29× 106 droplets/mL to 2.56 ± 0.22× 106 droplets/mL. The 4T1 cell viability decreased with increased exposure time and concentration of paclitaxel, but it was not affected by the presence of CNF-shelled PFP droplets. No hemolysis was observed at any concentration of CNF-shelled PFP droplets.Conclusion: CNF-shelled PFP droplets have the potential to be applied as drug carriers in ultrasound-mediated therapy.Keywords: cell viability, ultrasound-mediated therapy, paclitaxel, cellulose nanofibers, biocompatibility, 4T1, Pickering emulsionhttps://www.dovepress.com/cellulose-nanofiber-coated-perfluoropentane-droplets-fabrication-and-b-peer-reviewed-fulltext-article-IJNcell viabilityultrasound-mediated therapypaclitaxelcellulose nanofibersbiocompatibility4t1pickering emulsion. |
| spellingShingle | Loskutova K Torras M Zhao Y Svagan AJ Grishenkov D Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study International Journal of Nanomedicine cell viability ultrasound-mediated therapy paclitaxel cellulose nanofibers biocompatibility 4t1 pickering emulsion. |
| title | Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study |
| title_full | Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study |
| title_fullStr | Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study |
| title_full_unstemmed | Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study |
| title_short | Cellulose Nanofiber-Coated Perfluoropentane Droplets: Fabrication and Biocompatibility Study |
| title_sort | cellulose nanofiber coated perfluoropentane droplets fabrication and biocompatibility study |
| topic | cell viability ultrasound-mediated therapy paclitaxel cellulose nanofibers biocompatibility 4t1 pickering emulsion. |
| url | https://www.dovepress.com/cellulose-nanofiber-coated-perfluoropentane-droplets-fabrication-and-b-peer-reviewed-fulltext-article-IJN |
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