Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.)
Cadmium (Cd)-induced oxidative stress detrimentally affects hyperaccumulator growth, thereby diminishing the efficacy of phytoremediation technology aimed at Cd pollution abatement. In the domain of plant antioxidant mechanisms, the role of glutathione peroxidase (GPX) in conferring Cd tolerance to...
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MDPI AG
2024-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/15/3/366 |
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| author | Xiang Peng Tengfei Ma Kejin Song Xue Ji Lien Xiang Nan Chen Ronglei Zu Wenyi Xu Shunqin Zhu Wanhong Liu |
| author_facet | Xiang Peng Tengfei Ma Kejin Song Xue Ji Lien Xiang Nan Chen Ronglei Zu Wenyi Xu Shunqin Zhu Wanhong Liu |
| author_sort | Xiang Peng |
| collection | DOAJ |
| description | Cadmium (Cd)-induced oxidative stress detrimentally affects hyperaccumulator growth, thereby diminishing the efficacy of phytoremediation technology aimed at Cd pollution abatement. In the domain of plant antioxidant mechanisms, the role of glutathione peroxidase (GPX) in conferring Cd tolerance to tobacco (<i>Nicotiana tabacum</i>) remained unclear. Our investigation employed genome-wide analysis to identify 14 <i>NtGPX</i> genes in tobacco, revealing their organization into seven subgroups characterized by analogous conserved domain patterns. Notably, qPCR analysis highlighted <i>NtGPX8a</i> as markedly responsive to Cd<sup>2+</sup> stress. Subsequent exploration through yeast two-hybridization unveiled NtGPX8a’s utilization of thioredoxins AtTrxZ and AtTrxm2 as electron donors, and without interaction with AtTrx5. Introduction of <i>NtGPX8a</i> into <i>Escherichia coli</i> significantly ameliorated Cd-induced adverse effects on bacterial growth. Transgenic tobacco overexpressing <i>NtGPX8a</i> demonstrated significantly augmented activities of GPX, SOD, POD, and CAT under Cd<sup>2+</sup> stress compared to the wild type (WT). Conversely, these transgenic plants exhibited markedly reduced levels of MDA, H<sub>2</sub>O<sub>2</sub>, and proline. Intriguingly, the expression of <i>NtGPX8a</i> in both <i>E. coli</i> and transgenic tobacco led to increased Cd accumulation, confirming its dual role in enhancing Cd tolerance and accumulation. Consequently, <i>NtGPX8a</i> emerges as a promising candidate gene for engineering transgenic hyperaccumulators endowed with robust tolerance for Cd-contaminated phytoremediation. |
| first_indexed | 2024-04-24T18:14:50Z |
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| language | English |
| last_indexed | 2024-04-24T18:14:50Z |
| publishDate | 2024-03-01 |
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| series | Genes |
| spelling | doaj.art-8258c4fac80946c3aa1ea0c8992b34c52024-03-27T13:43:12ZengMDPI AGGenes2073-44252024-03-0115336610.3390/genes15030366Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.)Xiang Peng0Tengfei Ma1Kejin Song2Xue Ji3Lien Xiang4Nan Chen5Ronglei Zu6Wenyi Xu7Shunqin Zhu8Wanhong Liu9School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaCollege of Environmental Science & Engineering, China West Normal University, Nanchong 637009, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaSchool of Life Science, Southwest University, Chongqing 400715, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, ChinaCadmium (Cd)-induced oxidative stress detrimentally affects hyperaccumulator growth, thereby diminishing the efficacy of phytoremediation technology aimed at Cd pollution abatement. In the domain of plant antioxidant mechanisms, the role of glutathione peroxidase (GPX) in conferring Cd tolerance to tobacco (<i>Nicotiana tabacum</i>) remained unclear. Our investigation employed genome-wide analysis to identify 14 <i>NtGPX</i> genes in tobacco, revealing their organization into seven subgroups characterized by analogous conserved domain patterns. Notably, qPCR analysis highlighted <i>NtGPX8a</i> as markedly responsive to Cd<sup>2+</sup> stress. Subsequent exploration through yeast two-hybridization unveiled NtGPX8a’s utilization of thioredoxins AtTrxZ and AtTrxm2 as electron donors, and without interaction with AtTrx5. Introduction of <i>NtGPX8a</i> into <i>Escherichia coli</i> significantly ameliorated Cd-induced adverse effects on bacterial growth. Transgenic tobacco overexpressing <i>NtGPX8a</i> demonstrated significantly augmented activities of GPX, SOD, POD, and CAT under Cd<sup>2+</sup> stress compared to the wild type (WT). Conversely, these transgenic plants exhibited markedly reduced levels of MDA, H<sub>2</sub>O<sub>2</sub>, and proline. Intriguingly, the expression of <i>NtGPX8a</i> in both <i>E. coli</i> and transgenic tobacco led to increased Cd accumulation, confirming its dual role in enhancing Cd tolerance and accumulation. Consequently, <i>NtGPX8a</i> emerges as a promising candidate gene for engineering transgenic hyperaccumulators endowed with robust tolerance for Cd-contaminated phytoremediation.https://www.mdpi.com/2073-4425/15/3/366cadmiumtobaccoNtGPX8aoxidative stressgenome-wide analysis |
| spellingShingle | Xiang Peng Tengfei Ma Kejin Song Xue Ji Lien Xiang Nan Chen Ronglei Zu Wenyi Xu Shunqin Zhu Wanhong Liu Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.) Genes cadmium tobacco NtGPX8a oxidative stress genome-wide analysis |
| title | Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_full | Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_fullStr | Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_full_unstemmed | Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_short | Overexpression of <i>NtGPX8a</i> Improved Cadmium Accumulation and Tolerance in Tobacco (<i>Nicotiana tabacum</i> L.) |
| title_sort | overexpression of i ntgpx8a i improved cadmium accumulation and tolerance in tobacco i nicotiana tabacum i l |
| topic | cadmium tobacco NtGPX8a oxidative stress genome-wide analysis |
| url | https://www.mdpi.com/2073-4425/15/3/366 |
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