The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i>
The human pathogenic fungus <i>Aspergillus fumigatus</i> is readily eradicated by the innate immunity of immunocompetent human hosts, but can cause severe infections, such as invasive aspergillosis (IA), in immunocompromised individuals. During infection, the fungal redox homeostasis can...
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MDPI AG
2021-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/668 |
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| author | Jana Marie Boysen Nauman Saeed Thomas Wolf Gianni Panagiotou Falk Hillmann |
| author_facet | Jana Marie Boysen Nauman Saeed Thomas Wolf Gianni Panagiotou Falk Hillmann |
| author_sort | Jana Marie Boysen |
| collection | DOAJ |
| description | The human pathogenic fungus <i>Aspergillus fumigatus</i> is readily eradicated by the innate immunity of immunocompetent human hosts, but can cause severe infections, such as invasive aspergillosis (IA), in immunocompromised individuals. During infection, the fungal redox homeostasis can be challenged by reactive oxygen species (ROS), either derived from the oxidative burst of innate immune cells or the action of antifungal drugs. The peroxiredoxin Asp f3 was found to be essential to cause IA in mice, but how Asp f3 integrates with fungal redox homeostasis remains unknown. Here, we show that in vivo, Asp f3 acts as a sensor for ROS. While global transcription in fungal hyphae under minimal growth conditions was fully independent of Asp f3, a robust induction of the oxidative stress response required the presence of the peroxiredoxin. Hyphae devoid of Asp f3 failed to activate several redox active genes, like members of the gliotoxin biosynthesis gene cluster and integral members of the Afyap1 regulon, the central activator of the ROS defense machinery in fungi. Upon deletion of the <i>asp f3</i> gene Afyap1 displayed significantly reduced nuclear localization during ROS exposure, indicating that Asp f3 can act as an intracellular redox sensor for several target proteins. |
| first_indexed | 2024-03-10T11:50:14Z |
| format | Article |
| id | doaj.art-8259f6238fd14a4cb59d4f62320c4413 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:50:14Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-8259f6238fd14a4cb59d4f62320c44132023-11-21T17:45:52ZengMDPI AGGenes2073-44252021-04-0112566810.3390/genes12050668The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i>Jana Marie Boysen0Nauman Saeed1Thomas Wolf2Gianni Panagiotou3Falk Hillmann4Junior Research Group Evolution of Microbial Interactions, Leibniz-Institute for Natural Product Research and Infection Biology–Hans Knöll Institute (HKI), Beutenbergstr. 11a, 07745 Jena, GermanyJunior Research Group Evolution of Microbial Interactions, Leibniz-Institute for Natural Product Research and Infection Biology–Hans Knöll Institute (HKI), Beutenbergstr. 11a, 07745 Jena, GermanySystemsbiology and Bioinformatics, Leibniz-Institute for Natural Product Research and Infection Biology–Hans Knöll Institute, Beutenbergstr. 11a, 07745 Jena, GermanySystemsbiology and Bioinformatics, Leibniz-Institute for Natural Product Research and Infection Biology–Hans Knöll Institute, Beutenbergstr. 11a, 07745 Jena, GermanyJunior Research Group Evolution of Microbial Interactions, Leibniz-Institute for Natural Product Research and Infection Biology–Hans Knöll Institute (HKI), Beutenbergstr. 11a, 07745 Jena, GermanyThe human pathogenic fungus <i>Aspergillus fumigatus</i> is readily eradicated by the innate immunity of immunocompetent human hosts, but can cause severe infections, such as invasive aspergillosis (IA), in immunocompromised individuals. During infection, the fungal redox homeostasis can be challenged by reactive oxygen species (ROS), either derived from the oxidative burst of innate immune cells or the action of antifungal drugs. The peroxiredoxin Asp f3 was found to be essential to cause IA in mice, but how Asp f3 integrates with fungal redox homeostasis remains unknown. Here, we show that in vivo, Asp f3 acts as a sensor for ROS. While global transcription in fungal hyphae under minimal growth conditions was fully independent of Asp f3, a robust induction of the oxidative stress response required the presence of the peroxiredoxin. Hyphae devoid of Asp f3 failed to activate several redox active genes, like members of the gliotoxin biosynthesis gene cluster and integral members of the Afyap1 regulon, the central activator of the ROS defense machinery in fungi. Upon deletion of the <i>asp f3</i> gene Afyap1 displayed significantly reduced nuclear localization during ROS exposure, indicating that Asp f3 can act as an intracellular redox sensor for several target proteins.https://www.mdpi.com/2073-4425/12/5/668<i>Aspergillus fumigatus</i>peroxiredoxin Asp f3AfYap1transcriptomicsoxidative stress |
| spellingShingle | Jana Marie Boysen Nauman Saeed Thomas Wolf Gianni Panagiotou Falk Hillmann The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i> Genes <i>Aspergillus fumigatus</i> peroxiredoxin Asp f3 AfYap1 transcriptomics oxidative stress |
| title | The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i> |
| title_full | The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i> |
| title_fullStr | The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i> |
| title_full_unstemmed | The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i> |
| title_short | The Peroxiredoxin Asp f3 Acts as Redox Sensor in <i>Aspergillus fumigatus</i> |
| title_sort | peroxiredoxin asp f3 acts as redox sensor in i aspergillus fumigatus i |
| topic | <i>Aspergillus fumigatus</i> peroxiredoxin Asp f3 AfYap1 transcriptomics oxidative stress |
| url | https://www.mdpi.com/2073-4425/12/5/668 |
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