Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus
The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ-pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were tra...
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Academia Brasileira de Ciências
2010-09-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300004 |
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| author | Nelson R. Ferreira Maria Inez M. Sarquis Cláudio N. Alves Alberdan S. Santos |
| author_facet | Nelson R. Ferreira Maria Inez M. Sarquis Cláudio N. Alves Alberdan S. Santos |
| author_sort | Nelson R. Ferreira |
| collection | DOAJ |
| description | The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ-pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28"C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l-1 in 120g l-1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase capable of converting fructose into glucose. In this work, it focused the preference for glucose and, then, of fructose by A. flavus and the strategy used to produce HHMP.<br>Foram investigadas a hidrólise da sacarose e a preferência pela glicose frente à frutose no processo de produção do 5-hidroxi-2-hidroximetil-γ-pirona (HHMP) na presença de Aspergillus flavus IOC 3974 cultivado em meio líquido Czapeck. Quantidades de 0,5g de pelletes foram utilizadas como inóculo. Doze frascos cônicos de 250 ml contendo 100 ml de meio de culturacom diferentes concentrações de sacarose foram utilizados.Os microrganismos foram cultivados a 120 rpm e 28"C por 16 dias sem ajuste do pH. O maior rendimento do HHMP foi 26g l-1em 120g l-1de sacarose. Nestas condições, A. flavus, foi capaz de produzir uma invertase possibilitando a hidrólise de 65% da concentração total de sacarose em 24 horas, conjuntamente com a produção de uma isomerase que foi capaz de converter a frutose em glicose. Este trabalho está focalizado preferencialmente no consumo da glicose frente à frutose por A. flavus e na estratégia de produção do HHMP. |
| first_indexed | 2024-12-19T03:40:46Z |
| format | Article |
| id | doaj.art-825a12952b2b46349acb17170476376e |
| institution | Directory Open Access Journal |
| issn | 0001-3765 1678-2690 |
| language | English |
| last_indexed | 2024-12-19T03:40:46Z |
| publishDate | 2010-09-01 |
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| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-825a12952b2b46349acb17170476376e2022-12-21T20:37:13ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências0001-37651678-26902010-09-0182356957610.1590/S0001-37652010000300004Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavusNelson R. FerreiraMaria Inez M. SarquisCláudio N. AlvesAlberdan S. SantosThe sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ-pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28"C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l-1 in 120g l-1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase capable of converting fructose into glucose. In this work, it focused the preference for glucose and, then, of fructose by A. flavus and the strategy used to produce HHMP.<br>Foram investigadas a hidrólise da sacarose e a preferência pela glicose frente à frutose no processo de produção do 5-hidroxi-2-hidroximetil-γ-pirona (HHMP) na presença de Aspergillus flavus IOC 3974 cultivado em meio líquido Czapeck. Quantidades de 0,5g de pelletes foram utilizadas como inóculo. Doze frascos cônicos de 250 ml contendo 100 ml de meio de culturacom diferentes concentrações de sacarose foram utilizados.Os microrganismos foram cultivados a 120 rpm e 28"C por 16 dias sem ajuste do pH. O maior rendimento do HHMP foi 26g l-1em 120g l-1de sacarose. Nestas condições, A. flavus, foi capaz de produzir uma invertase possibilitando a hidrólise de 65% da concentração total de sacarose em 24 horas, conjuntamente com a produção de uma isomerase que foi capaz de converter a frutose em glicose. Este trabalho está focalizado preferencialmente no consumo da glicose frente à frutose por A. flavus e na estratégia de produção do HHMP.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300004Aspergillus flavusbiotransformaçãoácido kójicometabólito secundáriofrutose-isomeraseAspergillus flavusbiotransformationkojic acidsecondary metabolitefructose-isomerase |
| spellingShingle | Nelson R. Ferreira Maria Inez M. Sarquis Cláudio N. Alves Alberdan S. Santos Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus Anais da Academia Brasileira de Ciências Aspergillus flavus biotransformação ácido kójico metabólito secundário frutose-isomerase Aspergillus flavus biotransformation kojic acid secondary metabolite fructose-isomerase |
| title | Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus |
| title_full | Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus |
| title_fullStr | Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus |
| title_full_unstemmed | Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus |
| title_short | Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ-pirone by Aspergillus flavus |
| title_sort | biotransformation of sucrose into 5 hydroxy 2 hydroxymethyl 947 pirone by aspergillus flavus |
| topic | Aspergillus flavus biotransformação ácido kójico metabólito secundário frutose-isomerase Aspergillus flavus biotransformation kojic acid secondary metabolite fructose-isomerase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300004 |
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