Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats
The main goal of our research was to fabricate electrospun scaffolds from three different silk proteins—silk fibroin from <i>Bombyx mori</i> silkworm cocoons and two recombinant spidroins, rS2/12 and rS2/12-RGDS—and to perform a comparative analysis of the structure, biological propertie...
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MDPI AG
2021-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/10/1704 |
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| author | Liubov Safonova Maria Bobrova Anton Efimov Lyubov Davydova Timur Tenchurin Vladimir Bogush Olga Agapova Igor Agapov |
| author_facet | Liubov Safonova Maria Bobrova Anton Efimov Lyubov Davydova Timur Tenchurin Vladimir Bogush Olga Agapova Igor Agapov |
| author_sort | Liubov Safonova |
| collection | DOAJ |
| description | The main goal of our research was to fabricate electrospun scaffolds from three different silk proteins—silk fibroin from <i>Bombyx mori</i> silkworm cocoons and two recombinant spidroins, rS2/12 and rS2/12-RGDS—and to perform a comparative analysis of the structure, biological properties, and regenerative potential of the scaffolds in a full-thickness rat skin wound model. The surface and internal structures were investigated using scanning electron microscopy and scanning probe nanotomography. The structures of the scaffolds were similar. The average fiber diameter of the scaffolds was 315 ± 26 nm, the volume porosity was 94.5 ± 1.4%, the surface-to-volume ratio of the scaffolds was 25.4 ± 4.2 μm<sup>−1</sup> and the fiber surface roughness was 3.8 ± 0.6 nm. The scaffolds were characterized by a non-cytotoxicity effect and a high level of cytocompatibility with cells. The scaffolds also had high regenerative potential—the healing of the skin wound was accelerated by 19 days compared with the control. A histological analysis did not reveal any fragments of the experimental constructions or areas of inflammation. Thus, novel data on the structure and biological properties of the silk fibroin/spidroin electrospun scaffolds were obtained. |
| first_indexed | 2024-03-10T06:16:21Z |
| format | Article |
| id | doaj.art-825c2bf959444bf0b9a32665f95123c1 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T06:16:21Z |
| publishDate | 2021-10-01 |
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| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-825c2bf959444bf0b9a32665f95123c12023-11-22T19:40:03ZengMDPI AGPharmaceutics1999-49232021-10-011310170410.3390/pharmaceutics13101704Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in RatsLiubov Safonova0Maria Bobrova1Anton Efimov2Lyubov Davydova3Timur Tenchurin4Vladimir Bogush5Olga Agapova6Igor Agapov7Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaNational Research Center “Kurchatov Institute”, pl. Academician Kurchatov 1, 123182 Moscow, RussiaNational Research Center “Kurchatov Institute”, pl. Academician Kurchatov 1, 123182 Moscow, RussiaNational Research Center “Kurchatov Institute”, pl. Academician Kurchatov 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaThe main goal of our research was to fabricate electrospun scaffolds from three different silk proteins—silk fibroin from <i>Bombyx mori</i> silkworm cocoons and two recombinant spidroins, rS2/12 and rS2/12-RGDS—and to perform a comparative analysis of the structure, biological properties, and regenerative potential of the scaffolds in a full-thickness rat skin wound model. The surface and internal structures were investigated using scanning electron microscopy and scanning probe nanotomography. The structures of the scaffolds were similar. The average fiber diameter of the scaffolds was 315 ± 26 nm, the volume porosity was 94.5 ± 1.4%, the surface-to-volume ratio of the scaffolds was 25.4 ± 4.2 μm<sup>−1</sup> and the fiber surface roughness was 3.8 ± 0.6 nm. The scaffolds were characterized by a non-cytotoxicity effect and a high level of cytocompatibility with cells. The scaffolds also had high regenerative potential—the healing of the skin wound was accelerated by 19 days compared with the control. A histological analysis did not reveal any fragments of the experimental constructions or areas of inflammation. Thus, novel data on the structure and biological properties of the silk fibroin/spidroin electrospun scaffolds were obtained.https://www.mdpi.com/1999-4923/13/10/1704silk fibroinrecombinant spidroinelectrospinningscanning probe nanotomographyfull-thickness skin wound |
| spellingShingle | Liubov Safonova Maria Bobrova Anton Efimov Lyubov Davydova Timur Tenchurin Vladimir Bogush Olga Agapova Igor Agapov Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats Pharmaceutics silk fibroin recombinant spidroin electrospinning scanning probe nanotomography full-thickness skin wound |
| title | Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats |
| title_full | Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats |
| title_fullStr | Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats |
| title_full_unstemmed | Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats |
| title_short | Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats |
| title_sort | silk fibroin spidroin electrospun scaffolds for full thickness skin wound healing in rats |
| topic | silk fibroin recombinant spidroin electrospinning scanning probe nanotomography full-thickness skin wound |
| url | https://www.mdpi.com/1999-4923/13/10/1704 |
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