Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications
Poly-L-lactic acid (PLLA) aerogel-based scaffolds were obtained from physical PLLA gels containing cyclopentanone (CPO) or methyl benzoate (BzOMe) molecules. An innovative single step method of solvent extraction, using supercritical CO<sub>2</sub>, was used to achieve cylindrical monoli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2137 |
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| author | Ramona Orlacchio Simona Zuppolini Iriczalli Cruz-Maya Stefania Pragliola Anna Borriello Vincenzo Guarino Rosalba Fittipaldi Mariateresa Lettieri Vincenzo Venditto |
| author_facet | Ramona Orlacchio Simona Zuppolini Iriczalli Cruz-Maya Stefania Pragliola Anna Borriello Vincenzo Guarino Rosalba Fittipaldi Mariateresa Lettieri Vincenzo Venditto |
| author_sort | Ramona Orlacchio |
| collection | DOAJ |
| description | Poly-L-lactic acid (PLLA) aerogel-based scaffolds were obtained from physical PLLA gels containing cyclopentanone (CPO) or methyl benzoate (BzOMe) molecules. An innovative single step method of solvent extraction, using supercritical CO<sub>2</sub>, was used to achieve cylindrical monolithic aerogels. The pore distribution and size, analyzed by SEM microscopy, were found to be related to the crystalline forms present in the physical nodes that hold the gels together, the stable α’-form and the metastable co-crystalline ε-form, detected in the PLLA/BzOMe and PLLA/CPO aerogels, respectively. A higher mechanical compressive strength was found for the PLLA/CPO aerogels, which exhibit a more homogenous porosity. In vitro biocompatibility tests also indicated that monolithic PLLA/CPO aerogels exhibited greater cell viability than PLLA/BzOMe aerogels. An improved biocompatibility of PLLA/CPO monolithic aerogels was finally observed by coating the surface of the aerogels with polydopamine (PDA) obtained by the in situ polymerization of dopamine (DA). The synergistic effect of biodegradable polyester (PLLA) and the biomimetic interface (PDA) makes this new 3D porous scaffold, with porosity and mechanical properties that are tunable based on the solvent used in the preparation process, attractive for tissue engineering applications. |
| first_indexed | 2024-03-09T11:37:08Z |
| format | Article |
| id | doaj.art-82608229a4414ce7b62751073d27920c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:37:08Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-82608229a4414ce7b62751073d27920c2023-11-30T23:40:04ZengMDPI AGMolecules1420-30492022-03-01277213710.3390/molecules27072137Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering ApplicationsRamona Orlacchio0Simona Zuppolini1Iriczalli Cruz-Maya2Stefania Pragliola3Anna Borriello4Vincenzo Guarino5Rosalba Fittipaldi6Mariateresa Lettieri7Vincenzo Venditto8Department of Chemistry and Biology, INSTM Research Unit, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, SA, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, P.le Fermi, 80055 Portici, NA, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, P.le Fermi, 80055 Portici, NA, ItalyDepartment of Chemistry and Biology, INSTM Research Unit, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, SA, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, P.le Fermi, 80055 Portici, NA, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, P.le Fermi, 80055 Portici, NA, ItalyCNR—SPIN, Via Giovanni Paolo II 132, 84084 Fisciano, SA, ItalyCNR—SPIN, Via Giovanni Paolo II 132, 84084 Fisciano, SA, ItalyDepartment of Chemistry and Biology, INSTM Research Unit, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, SA, ItalyPoly-L-lactic acid (PLLA) aerogel-based scaffolds were obtained from physical PLLA gels containing cyclopentanone (CPO) or methyl benzoate (BzOMe) molecules. An innovative single step method of solvent extraction, using supercritical CO<sub>2</sub>, was used to achieve cylindrical monolithic aerogels. The pore distribution and size, analyzed by SEM microscopy, were found to be related to the crystalline forms present in the physical nodes that hold the gels together, the stable α’-form and the metastable co-crystalline ε-form, detected in the PLLA/BzOMe and PLLA/CPO aerogels, respectively. A higher mechanical compressive strength was found for the PLLA/CPO aerogels, which exhibit a more homogenous porosity. In vitro biocompatibility tests also indicated that monolithic PLLA/CPO aerogels exhibited greater cell viability than PLLA/BzOMe aerogels. An improved biocompatibility of PLLA/CPO monolithic aerogels was finally observed by coating the surface of the aerogels with polydopamine (PDA) obtained by the in situ polymerization of dopamine (DA). The synergistic effect of biodegradable polyester (PLLA) and the biomimetic interface (PDA) makes this new 3D porous scaffold, with porosity and mechanical properties that are tunable based on the solvent used in the preparation process, attractive for tissue engineering applications.https://www.mdpi.com/1420-3049/27/7/2137poly-lactic acidpolydopamineaerogelsscaffolds |
| spellingShingle | Ramona Orlacchio Simona Zuppolini Iriczalli Cruz-Maya Stefania Pragliola Anna Borriello Vincenzo Guarino Rosalba Fittipaldi Mariateresa Lettieri Vincenzo Venditto Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications Molecules poly-lactic acid polydopamine aerogels scaffolds |
| title | Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications |
| title_full | Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications |
| title_fullStr | Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications |
| title_full_unstemmed | Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications |
| title_short | Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications |
| title_sort | polydopamine coated poly lactic acid aerogels as scaffolds for tissue engineering applications |
| topic | poly-lactic acid polydopamine aerogels scaffolds |
| url | https://www.mdpi.com/1420-3049/27/7/2137 |
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