Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study
Aim: The primary objective of this pilot study was to evaluate the effect of non-surgical periodontal treatment. The secondary aim was to evaluate the effect of dietary therapy on both parameters of oxidative stress in leukocytes and leukocyte-endothelial cell interactions in an obese population. Me...
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MDPI AG
2020-07-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/7/2117 |
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| author | Mayte Martínez-Herrera Zaida Abad-Jiménez Francisco Javier Silvestre Sandra López-Domènech Javier Silvestre-Rangil Cecilia Fabiana Márquez-Arrico Víctor M. Víctor Milagros Rocha |
| author_facet | Mayte Martínez-Herrera Zaida Abad-Jiménez Francisco Javier Silvestre Sandra López-Domènech Javier Silvestre-Rangil Cecilia Fabiana Márquez-Arrico Víctor M. Víctor Milagros Rocha |
| author_sort | Mayte Martínez-Herrera |
| collection | DOAJ |
| description | Aim: The primary objective of this pilot study was to evaluate the effect of non-surgical periodontal treatment. The secondary aim was to evaluate the effect of dietary therapy on both parameters of oxidative stress in leukocytes and leukocyte-endothelial cell interactions in an obese population. Methods: This was a pilot study with a before-and-after design. Forty-nine obese subjects with periodontitis were randomized by means of the minimization method and assigned to one of two groups, one of which underwent dietary therapy while the other did not. All the subjects underwent non-surgical periodontal treatment. We determined periodontal, inflammatory and oxidative stress parameters—total reactive oxygen species (ROS), superoxide production, intracellular Ca<sup>2+</sup>, mitochondrial membrane potential and superoxide dismutase (SOD) activity. We also evaluated interactions between leukocytes and endothelium cells—velocity, rolling flux and adhesion—at baseline and 12 weeks after intervention. Results: Periodontal treatment improved the periodontal health of all the patients, with a reduction in serum retinol-binding protein 4 (RBP4), total superoxide production and cytosolic Ca<sup>2+</sup> in leukocytes. In the patients undergoing dietary therapy, there were less leukocyte adhesion to the endothelium, an effect that was accompanied by a decrease in TNFα, P-selectin and total ROS and an increase in SOD activity. Conclusions: Whereas non-surgical periodontal treatment induces an improvement in leukocyte homeostasis, dietary therapy as an adjuvant reduces systemic inflammation and increases antioxidant status which, in turn, modulates leukocyte-endothelium dynamics. |
| first_indexed | 2024-03-10T18:40:39Z |
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| language | English |
| last_indexed | 2024-03-10T18:40:39Z |
| publishDate | 2020-07-01 |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-8262d14abffb417daad0e63adf91f7aa2023-11-20T05:52:44ZengMDPI AGJournal of Clinical Medicine2077-03832020-07-0197211710.3390/jcm9072117Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot StudyMayte Martínez-Herrera0Zaida Abad-Jiménez1Francisco Javier Silvestre2Sandra López-Domènech3Javier Silvestre-Rangil4Cecilia Fabiana Márquez-Arrico5Víctor M. Víctor6Milagros Rocha7Department of Stomatology, University of Valencia, Gascó i Oliag 1, 46010 Valencia, SpainDepartment of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Av. Gaspar Aguilar 90, 46017 Valencia, SpainDepartment of Stomatology, University of Valencia, Gascó i Oliag 1, 46010 Valencia, SpainDepartment of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Av. Gaspar Aguilar 90, 46017 Valencia, SpainDepartment of Stomatology, University Hospital Doctor Peset-FISABIO, Av. Gaspar Aguilar 90, 46017 Valencia, SpainDepartment of Stomatology, University of Valencia, Gascó i Oliag 1, 46010 Valencia, SpainDepartment of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Av. Gaspar Aguilar 90, 46017 Valencia, SpainDepartment of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Av. Gaspar Aguilar 90, 46017 Valencia, SpainAim: The primary objective of this pilot study was to evaluate the effect of non-surgical periodontal treatment. The secondary aim was to evaluate the effect of dietary therapy on both parameters of oxidative stress in leukocytes and leukocyte-endothelial cell interactions in an obese population. Methods: This was a pilot study with a before-and-after design. Forty-nine obese subjects with periodontitis were randomized by means of the minimization method and assigned to one of two groups, one of which underwent dietary therapy while the other did not. All the subjects underwent non-surgical periodontal treatment. We determined periodontal, inflammatory and oxidative stress parameters—total reactive oxygen species (ROS), superoxide production, intracellular Ca<sup>2+</sup>, mitochondrial membrane potential and superoxide dismutase (SOD) activity. We also evaluated interactions between leukocytes and endothelium cells—velocity, rolling flux and adhesion—at baseline and 12 weeks after intervention. Results: Periodontal treatment improved the periodontal health of all the patients, with a reduction in serum retinol-binding protein 4 (RBP4), total superoxide production and cytosolic Ca<sup>2+</sup> in leukocytes. In the patients undergoing dietary therapy, there were less leukocyte adhesion to the endothelium, an effect that was accompanied by a decrease in TNFα, P-selectin and total ROS and an increase in SOD activity. Conclusions: Whereas non-surgical periodontal treatment induces an improvement in leukocyte homeostasis, dietary therapy as an adjuvant reduces systemic inflammation and increases antioxidant status which, in turn, modulates leukocyte-endothelium dynamics.https://www.mdpi.com/2077-0383/9/7/2117periodontitisreactive oxygen speciesoxidative stressendothelial dysfunctionperiodontal treatmentdietary therapy |
| spellingShingle | Mayte Martínez-Herrera Zaida Abad-Jiménez Francisco Javier Silvestre Sandra López-Domènech Javier Silvestre-Rangil Cecilia Fabiana Márquez-Arrico Víctor M. Víctor Milagros Rocha Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study Journal of Clinical Medicine periodontitis reactive oxygen species oxidative stress endothelial dysfunction periodontal treatment dietary therapy |
| title | Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study |
| title_full | Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study |
| title_fullStr | Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study |
| title_full_unstemmed | Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study |
| title_short | Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study |
| title_sort | effect of non surgical periodontal treatment on oxidative stress markers in leukocytes and their interaction with the endothelium in obese subjects with periodontitis a pilot study |
| topic | periodontitis reactive oxygen species oxidative stress endothelial dysfunction periodontal treatment dietary therapy |
| url | https://www.mdpi.com/2077-0383/9/7/2117 |
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