Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape
Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglyc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01352/full |
| _version_ | 1818291221373648896 |
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| author | André S. G. Lorenzoni Giordanni C. Dantas Tessa Bergsma Henrique Ferreira Dirk-Jan Scheffers |
| author_facet | André S. G. Lorenzoni Giordanni C. Dantas Tessa Bergsma Henrique Ferreira Dirk-Jan Scheffers |
| author_sort | André S. G. Lorenzoni |
| collection | DOAJ |
| description | Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Δmin phenotype observed in other bacteria deleted for min components: minicells and short filamentation. In addition we noticed the formation of branches, which is similar to what was previously described for Escherichia coli deleted for either min or for several low molecular weight penicillin-binding proteins (PBPs). The branching phenotype was medium dependent and probably linked to gluconeogenic growth. We complemented the minC gene by integrating gfp-minC into the amy locus. Xac complemented strains displayed a wild-type phenotype. In addition, GFP-MinC oscillated from pole to pole, similar to MinCD oscillations observed in E. coli and more recently in Synechococcus elongatus. Further investigation of the branching phenotype revealed that in branching cells nucleoid organization, divisome formation and peptidoglycan incorporation were disrupted. |
| first_indexed | 2024-12-13T02:40:37Z |
| format | Article |
| id | doaj.art-8263a944bc164b0f90306fa4f2e70eaa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T02:40:37Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8263a944bc164b0f90306fa4f2e70eaa2022-12-22T00:02:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-07-01810.3389/fmicb.2017.01352276999Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and ShapeAndré S. G. Lorenzoni0Giordanni C. Dantas1Tessa Bergsma2Henrique Ferreira3Dirk-Jan Scheffers4Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, NetherlandsDepartamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual PaulistaRio Claro, BrazilDepartment of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, NetherlandsDepartamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual PaulistaRio Claro, BrazilDepartment of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, NetherlandsXanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Δmin phenotype observed in other bacteria deleted for min components: minicells and short filamentation. In addition we noticed the formation of branches, which is similar to what was previously described for Escherichia coli deleted for either min or for several low molecular weight penicillin-binding proteins (PBPs). The branching phenotype was medium dependent and probably linked to gluconeogenic growth. We complemented the minC gene by integrating gfp-minC into the amy locus. Xac complemented strains displayed a wild-type phenotype. In addition, GFP-MinC oscillated from pole to pole, similar to MinCD oscillations observed in E. coli and more recently in Synechococcus elongatus. Further investigation of the branching phenotype revealed that in branching cells nucleoid organization, divisome formation and peptidoglycan incorporation were disrupted.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01352/fullXanthomonas citriMinCFtsZZapAParBpeptidoglycan |
| spellingShingle | André S. G. Lorenzoni Giordanni C. Dantas Tessa Bergsma Henrique Ferreira Dirk-Jan Scheffers Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape Frontiers in Microbiology Xanthomonas citri MinC FtsZ ZapA ParB peptidoglycan |
| title | Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape |
| title_full | Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape |
| title_fullStr | Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape |
| title_full_unstemmed | Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape |
| title_short | Xanthomonas citri MinC Oscillates from Pole to Pole to Ensure Proper Cell Division and Shape |
| title_sort | xanthomonas citri minc oscillates from pole to pole to ensure proper cell division and shape |
| topic | Xanthomonas citri MinC FtsZ ZapA ParB peptidoglycan |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01352/full |
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