Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma
Abstract Background Primary open angle glaucoma (POAG) is a multifactorial disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death. An increasing number of studies have suggested that long noncoding RNAs (lncRNAs) have the ability to regulate gene express...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-03-01
|
| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12886-020-01365-5 |
| _version_ | 1818499867672051712 |
|---|---|
| author | Minwen Zhou Bing Lu Wei Tan Mingshui Fu |
| author_facet | Minwen Zhou Bing Lu Wei Tan Mingshui Fu |
| author_sort | Minwen Zhou |
| collection | DOAJ |
| description | Abstract Background Primary open angle glaucoma (POAG) is a multifactorial disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death. An increasing number of studies have suggested that long noncoding RNAs (lncRNAs) have the ability to regulate gene expression; however, thus far, the mechanisms and functions of lncRNAs in the development of POAG are still unclear. Methods Using the data from Gene Expression Omnibus (GEO), differentially expressed lncRNAs and differentially expressed mRNAs between POAG patients and controls were identified. Then, the lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network was constructed, and the key lncRNAs in POAG were identified. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to assess the enriched biological functions of mRNA in the ceRNA network. Results During this study, a POAG-related ceRNA network with 37 miRNA nodes, 248 lncRNA nodes, 178 mRNA nodes, and 1985 edges was constructed. In addition, four lncRNAs (DNAJC27-AS1, AF121898, OIP5-AS1, and SNX29P2) were established as hub RNAs in this ceRNA network. The functional assay showed that 18 GO terms and 17 pathways were enriched. Conclusion This study provides novel insights into the lncRNA-related ceRNA network in POAG, and the four lncRNAs were identified in the development of POAG. |
| first_indexed | 2024-12-10T20:35:27Z |
| format | Article |
| id | doaj.art-82685af724a243338a709d0e862a8d78 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-10T20:35:27Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-82685af724a243338a709d0e862a8d782022-12-22T01:34:33ZengBMCBMC Ophthalmology1471-24152020-03-012011910.1186/s12886-020-01365-5Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucomaMinwen Zhou0Bing Lu1Wei Tan2Mingshui Fu3Department of Ophthalmology, Shanghai General Hospital (Shanghai First People’s Hospital), Shanghai Jiao Tong University School of MedicineDepartment of Ophthalmology, Shanghai General Hospital (Shanghai First People’s Hospital), Shanghai Jiao Tong University School of MedicineThe Department of Ophthalmology, The Third Affiliated Hospital of Zunyi Medical UniversityDepartment of Ophthalmology, Shanghai General Hospital (Shanghai First People’s Hospital), Shanghai Jiao Tong University School of MedicineAbstract Background Primary open angle glaucoma (POAG) is a multifactorial disorder characterized by a progressive permanent degeneration of retinal ganglion cell (RGCs) death. An increasing number of studies have suggested that long noncoding RNAs (lncRNAs) have the ability to regulate gene expression; however, thus far, the mechanisms and functions of lncRNAs in the development of POAG are still unclear. Methods Using the data from Gene Expression Omnibus (GEO), differentially expressed lncRNAs and differentially expressed mRNAs between POAG patients and controls were identified. Then, the lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network was constructed, and the key lncRNAs in POAG were identified. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to assess the enriched biological functions of mRNA in the ceRNA network. Results During this study, a POAG-related ceRNA network with 37 miRNA nodes, 248 lncRNA nodes, 178 mRNA nodes, and 1985 edges was constructed. In addition, four lncRNAs (DNAJC27-AS1, AF121898, OIP5-AS1, and SNX29P2) were established as hub RNAs in this ceRNA network. The functional assay showed that 18 GO terms and 17 pathways were enriched. Conclusion This study provides novel insights into the lncRNA-related ceRNA network in POAG, and the four lncRNAs were identified in the development of POAG.http://link.springer.com/article/10.1186/s12886-020-01365-5Primary open angle glaucomaLong noncoding RNAlncRNA-miRNA-mRNA regulatory network |
| spellingShingle | Minwen Zhou Bing Lu Wei Tan Mingshui Fu Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma BMC Ophthalmology Primary open angle glaucoma Long noncoding RNA lncRNA-miRNA-mRNA regulatory network |
| title | Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma |
| title_full | Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma |
| title_fullStr | Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma |
| title_full_unstemmed | Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma |
| title_short | Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma |
| title_sort | identification of lncrna mirna mrna regulatory network associated with primary open angle glaucoma |
| topic | Primary open angle glaucoma Long noncoding RNA lncRNA-miRNA-mRNA regulatory network |
| url | http://link.springer.com/article/10.1186/s12886-020-01365-5 |
| work_keys_str_mv | AT minwenzhou identificationoflncrnamirnamrnaregulatorynetworkassociatedwithprimaryopenangleglaucoma AT binglu identificationoflncrnamirnamrnaregulatorynetworkassociatedwithprimaryopenangleglaucoma AT weitan identificationoflncrnamirnamrnaregulatorynetworkassociatedwithprimaryopenangleglaucoma AT mingshuifu identificationoflncrnamirnamrnaregulatorynetworkassociatedwithprimaryopenangleglaucoma |