pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188
Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was deter...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2012-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-11-0215 |
| _version_ | 1811291272044871680 |
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| author | Isolde Francis Annick De Keyser Philippe De Backer Carmen Simón-Mateo Jutta Kalkus Ine Pertry Wilson Ardiles-Diaz Riet De Rycke Olivier M. Vandeputte Mondher El Jaziri Marcelle Holsters Danny Vereecke |
| author_facet | Isolde Francis Annick De Keyser Philippe De Backer Carmen Simón-Mateo Jutta Kalkus Ine Pertry Wilson Ardiles-Diaz Riet De Rycke Olivier M. Vandeputte Mondher El Jaziri Marcelle Holsters Danny Vereecke |
| author_sort | Isolde Francis |
| collection | DOAJ |
| description | Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi_086 and pFi_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actinobacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, att, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host. |
| first_indexed | 2024-04-13T04:26:56Z |
| format | Article |
| id | doaj.art-8269e1b65adb4838b5915a0d3ed00689 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:56Z |
| publishDate | 2012-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-8269e1b65adb4838b5915a0d3ed006892022-12-22T03:02:30ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062012-05-0125563764710.1094/MPMI-08-11-0215pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188Isolde FrancisAnnick De KeyserPhilippe De BackerCarmen Simón-MateoJutta KalkusIne PertryWilson Ardiles-DiazRiet De RyckeOlivier M. VandeputteMondher El JaziriMarcelle HolstersDanny VereeckeRhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi_086 and pFi_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actinobacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, att, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host.https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-11-0215 |
| spellingShingle | Isolde Francis Annick De Keyser Philippe De Backer Carmen Simón-Mateo Jutta Kalkus Ine Pertry Wilson Ardiles-Diaz Riet De Rycke Olivier M. Vandeputte Mondher El Jaziri Marcelle Holsters Danny Vereecke pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188 Molecular Plant-Microbe Interactions |
| title | pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188 |
| title_full | pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188 |
| title_fullStr | pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188 |
| title_full_unstemmed | pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188 |
| title_short | pFiD188, the Linear Virulence Plasmid of Rhodococcus fascians D188 |
| title_sort | pfid188 the linear virulence plasmid of rhodococcus fascians d188 |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-11-0215 |
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