Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli
Currently, Streptococcus pneumoniae is responsible for over 14 million cases of pneumonia worldwide annually, and over 1 million deaths, the majority of them children. The major determinant for pathogenesis is a polysaccharide capsule that is variable and is used to distinguish strains based on thei...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2016-01-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150243 |
| _version_ | 1818605813934063616 |
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| author | Emily J. Kay Laura E. Yates Vanessa S. Terra Jon Cuccui Brendan W. Wren |
| author_facet | Emily J. Kay Laura E. Yates Vanessa S. Terra Jon Cuccui Brendan W. Wren |
| author_sort | Emily J. Kay |
| collection | DOAJ |
| description | Currently, Streptococcus pneumoniae is responsible for over 14 million cases of pneumonia worldwide annually, and over 1 million deaths, the majority of them children. The major determinant for pathogenesis is a polysaccharide capsule that is variable and is used to distinguish strains based on their serotype. The capsule forms the basis of the pneumococcal polysaccharide vaccine (PPV23) that contains purified capsular polysaccharide from 23 serotypes, and the pneumococcal conjugate vaccine (PCV13), containing 13 common serotypes conjugated to CRM197 (mutant diphtheria toxin). Purified capsule from S. pneumoniae is required for pneumococcal conjugate vaccine production, and costs can be prohibitively high, limiting accessibility of the vaccine in low-income countries. In this study, we demonstrate the recombinant expression of the capsule-encoding locus from four different serotypes of S. pneumoniae within Escherichia coli. Furthermore, we attempt to identify the minimum set of genes necessary to reliably and efficiently express these capsules heterologously. These E. coli strains could be used to produce a supply of S. pneumoniae serotype-specific capsules without the need to culture pathogenic bacteria. Additionally, these strains could be applied to synthetic glycobiological applications: recombinant vaccine production using E. coli outer membrane vesicles or coupling to proteins using protein glycan coupling technology. |
| first_indexed | 2024-12-16T14:00:56Z |
| format | Article |
| id | doaj.art-826a99e70e3941628ce2e3f051f50a1b |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-16T14:00:56Z |
| publishDate | 2016-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-826a99e70e3941628ce2e3f051f50a1b2022-12-21T22:29:06ZengThe Royal SocietyOpen Biology2046-24412016-01-016410.1098/rsob.150243150243Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coliEmily J. KayLaura E. YatesVanessa S. TerraJon CuccuiBrendan W. WrenCurrently, Streptococcus pneumoniae is responsible for over 14 million cases of pneumonia worldwide annually, and over 1 million deaths, the majority of them children. The major determinant for pathogenesis is a polysaccharide capsule that is variable and is used to distinguish strains based on their serotype. The capsule forms the basis of the pneumococcal polysaccharide vaccine (PPV23) that contains purified capsular polysaccharide from 23 serotypes, and the pneumococcal conjugate vaccine (PCV13), containing 13 common serotypes conjugated to CRM197 (mutant diphtheria toxin). Purified capsule from S. pneumoniae is required for pneumococcal conjugate vaccine production, and costs can be prohibitively high, limiting accessibility of the vaccine in low-income countries. In this study, we demonstrate the recombinant expression of the capsule-encoding locus from four different serotypes of S. pneumoniae within Escherichia coli. Furthermore, we attempt to identify the minimum set of genes necessary to reliably and efficiently express these capsules heterologously. These E. coli strains could be used to produce a supply of S. pneumoniae serotype-specific capsules without the need to culture pathogenic bacteria. Additionally, these strains could be applied to synthetic glycobiological applications: recombinant vaccine production using E. coli outer membrane vesicles or coupling to proteins using protein glycan coupling technology.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150243streptococcus pneumoniaesynthetic glycobiologyglycoengineeringvaccinecapsular polysaccharide |
| spellingShingle | Emily J. Kay Laura E. Yates Vanessa S. Terra Jon Cuccui Brendan W. Wren Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli Open Biology streptococcus pneumoniae synthetic glycobiology glycoengineering vaccine capsular polysaccharide |
| title | Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli |
| title_full | Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli |
| title_fullStr | Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli |
| title_full_unstemmed | Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli |
| title_short | Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli |
| title_sort | recombinant expression of streptococcus pneumoniae capsular polysaccharides in escherichia coli |
| topic | streptococcus pneumoniae synthetic glycobiology glycoengineering vaccine capsular polysaccharide |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150243 |
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