Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis
Systemic sclerosis (SSc) is a rare systemic autoimmune disorder marked by high morbidity and increased risk of mortality. Our study aimed to analyze metabolomic profiles of plasma from SSc patients by using targeted and untargeted metabolomics approaches. Furthermore, we aimed to detect biochemical...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/3/607 |
| _version_ | 1797472767071748096 |
|---|---|
| author | Thomas Bögl Franz Mlynek Markus Himmelsbach Norbert Sepp Wolfgang Buchberger Marija Geroldinger-Simić |
| author_facet | Thomas Bögl Franz Mlynek Markus Himmelsbach Norbert Sepp Wolfgang Buchberger Marija Geroldinger-Simić |
| author_sort | Thomas Bögl |
| collection | DOAJ |
| description | Systemic sclerosis (SSc) is a rare systemic autoimmune disorder marked by high morbidity and increased risk of mortality. Our study aimed to analyze metabolomic profiles of plasma from SSc patients by using targeted and untargeted metabolomics approaches. Furthermore, we aimed to detect biochemical mechanisms relevant to the pathophysiology of SSc. Experiments were performed using high-performance liquid chromatography coupled to mass spectrometry technology. The investigation of plasma samples from SSc patients (<i>n</i> = 52) compared to a control group (<i>n</i> = 48) allowed us to identify four different dysfunctional metabolic mechanisms, which can be assigned to the kynurenine pathway, the urea cycle, lipid metabolism, and the gut microbiome. These significantly altered metabolic pathways are associated with inflammation, vascular damage, fibrosis, and gut dysbiosis and might be relevant for the pathophysiology of SSc. Further studies are needed to explore the role of these metabolomic networks as possible therapeutic targets of SSc. |
| first_indexed | 2024-03-09T20:05:54Z |
| format | Article |
| id | doaj.art-826e5e2b4a9b4199bcfdae78b560ebde |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:05:54Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-826e5e2b4a9b4199bcfdae78b560ebde2023-11-24T00:32:42ZengMDPI AGBiomedicines2227-90592022-03-0110360710.3390/biomedicines10030607Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic SclerosisThomas Bögl0Franz Mlynek1Markus Himmelsbach2Norbert Sepp3Wolfgang Buchberger4Marija Geroldinger-Simić5Institute of Analytical and General Chemistry, Johannes Kepler University Linz, 4040 Linz, AustriaInstitute of Analytical and General Chemistry, Johannes Kepler University Linz, 4040 Linz, AustriaInstitute of Analytical and General Chemistry, Johannes Kepler University Linz, 4040 Linz, AustriaDepartment of Dermatology, Ordensklinikum Linz Elisabethinen, 4020 Linz, AustriaInstitute of Analytical and General Chemistry, Johannes Kepler University Linz, 4040 Linz, AustriaDepartment of Dermatology, Ordensklinikum Linz Elisabethinen, 4020 Linz, AustriaSystemic sclerosis (SSc) is a rare systemic autoimmune disorder marked by high morbidity and increased risk of mortality. Our study aimed to analyze metabolomic profiles of plasma from SSc patients by using targeted and untargeted metabolomics approaches. Furthermore, we aimed to detect biochemical mechanisms relevant to the pathophysiology of SSc. Experiments were performed using high-performance liquid chromatography coupled to mass spectrometry technology. The investigation of plasma samples from SSc patients (<i>n</i> = 52) compared to a control group (<i>n</i> = 48) allowed us to identify four different dysfunctional metabolic mechanisms, which can be assigned to the kynurenine pathway, the urea cycle, lipid metabolism, and the gut microbiome. These significantly altered metabolic pathways are associated with inflammation, vascular damage, fibrosis, and gut dysbiosis and might be relevant for the pathophysiology of SSc. Further studies are needed to explore the role of these metabolomic networks as possible therapeutic targets of SSc.https://www.mdpi.com/2227-9059/10/3/607systemic sclerosismetabolomicsLC-MS/MSion mobilitykynurenine pathwayurea cycle |
| spellingShingle | Thomas Bögl Franz Mlynek Markus Himmelsbach Norbert Sepp Wolfgang Buchberger Marija Geroldinger-Simić Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis Biomedicines systemic sclerosis metabolomics LC-MS/MS ion mobility kynurenine pathway urea cycle |
| title | Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis |
| title_full | Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis |
| title_fullStr | Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis |
| title_full_unstemmed | Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis |
| title_short | Plasma Metabolomic Profiling Reveals Four Possibly Disrupted Mechanisms in Systemic Sclerosis |
| title_sort | plasma metabolomic profiling reveals four possibly disrupted mechanisms in systemic sclerosis |
| topic | systemic sclerosis metabolomics LC-MS/MS ion mobility kynurenine pathway urea cycle |
| url | https://www.mdpi.com/2227-9059/10/3/607 |
| work_keys_str_mv | AT thomasbogl plasmametabolomicprofilingrevealsfourpossiblydisruptedmechanismsinsystemicsclerosis AT franzmlynek plasmametabolomicprofilingrevealsfourpossiblydisruptedmechanismsinsystemicsclerosis AT markushimmelsbach plasmametabolomicprofilingrevealsfourpossiblydisruptedmechanismsinsystemicsclerosis AT norbertsepp plasmametabolomicprofilingrevealsfourpossiblydisruptedmechanismsinsystemicsclerosis AT wolfgangbuchberger plasmametabolomicprofilingrevealsfourpossiblydisruptedmechanismsinsystemicsclerosis AT marijageroldingersimic plasmametabolomicprofilingrevealsfourpossiblydisruptedmechanismsinsystemicsclerosis |