Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae
<p><strong><em>Introduction</em></strong>: <em>Streptococcus pneumoniae </em>cause morbidity and mortality in infants and younger children. Because of high prevalence of penicillin resistance, rapid and reliable diagnostic techniques for penicillin non-...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-11-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | http://jmb.tums.ac.ir/index.php/jmb/article/view/60 |
| _version_ | 1818057426111299584 |
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| author | Ali Ahmadi Farzaneh khodaei Maryam Esghaei Gholamreza Irajian Malihe Talebi |
| author_facet | Ali Ahmadi Farzaneh khodaei Maryam Esghaei Gholamreza Irajian Malihe Talebi |
| author_sort | Ali Ahmadi |
| collection | DOAJ |
| description | <p><strong><em>Introduction</em></strong>: <em>Streptococcus pneumoniae </em>cause morbidity and mortality in infants and younger children. Because of high prevalence of penicillin resistance, rapid and reliable diagnostic techniques for penicillin non-susceptible <em>S. pneumoniae </em>(PNSSP) are important for prevention and treatment. We investigated the association of the restriction length polymorphism (RFLP) patterns for <em>pbp2b </em>to distinguish between penicillin susceptible and resistant <em>S</em>. <em>pneumoniae </em>isolates.</p><p><strong><em>M</em></strong><strong><em>e</em></strong><strong><em>thods</em></strong>: In this study, a total of 70 pneumococcal isolates were collected from different clinical sources. MIC of these isolates was determined and <em>pbp</em>2b gene was amplified by PCR and they were digested by HaeІІІ enzyme.</p><p><strong><em>Results</em></strong><strong>: </strong>Of the 70 isolates, 86% (60) and 14% (10) pneumococcal isolates were found to be PNSSP (penicillin intermediate <em>S. pneumoniae </em>(PISP) and penicillin resistant <em>S. pneumoniae </em>(PRSP)) and penicillin susceptible <em>S. pneumoniae </em>(PSSP). In addition, 10 RFLP patterns (A-J) which were based on the HaeІІІ digestion of <em>pbp2b </em>gene were observed. All PSSP isolates showed that they belonged to pattern D, whereas, all PNSSP showed 10 different patterns.</p><p><strong><em>Conclusion</em>: </strong>In general, the present study suggests that RFLP can be a powerful tool in differentiation between the penicillin resistant and susceptible strains.</p> |
| first_indexed | 2024-12-10T12:44:33Z |
| format | Article |
| id | doaj.art-826e843bec8a4b01aad431dfe576e0b5 |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-12-10T12:44:33Z |
| publishDate | 2015-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-826e843bec8a4b01aad431dfe576e0b52022-12-22T01:48:26ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-11-0141-2152053Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniaeAli Ahmadi0Farzaneh khodaei1Maryam Esghaei2Gholamreza Irajian3Malihe Talebi4Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR IranDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences , Tehran, IR IranDepartment of virology, Iran University of medical science. Tehran, IR IranDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences , Tehran, IR IranDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences , Tehran, IR Iran<p><strong><em>Introduction</em></strong>: <em>Streptococcus pneumoniae </em>cause morbidity and mortality in infants and younger children. Because of high prevalence of penicillin resistance, rapid and reliable diagnostic techniques for penicillin non-susceptible <em>S. pneumoniae </em>(PNSSP) are important for prevention and treatment. We investigated the association of the restriction length polymorphism (RFLP) patterns for <em>pbp2b </em>to distinguish between penicillin susceptible and resistant <em>S</em>. <em>pneumoniae </em>isolates.</p><p><strong><em>M</em></strong><strong><em>e</em></strong><strong><em>thods</em></strong>: In this study, a total of 70 pneumococcal isolates were collected from different clinical sources. MIC of these isolates was determined and <em>pbp</em>2b gene was amplified by PCR and they were digested by HaeІІІ enzyme.</p><p><strong><em>Results</em></strong><strong>: </strong>Of the 70 isolates, 86% (60) and 14% (10) pneumococcal isolates were found to be PNSSP (penicillin intermediate <em>S. pneumoniae </em>(PISP) and penicillin resistant <em>S. pneumoniae </em>(PRSP)) and penicillin susceptible <em>S. pneumoniae </em>(PSSP). In addition, 10 RFLP patterns (A-J) which were based on the HaeІІІ digestion of <em>pbp2b </em>gene were observed. All PSSP isolates showed that they belonged to pattern D, whereas, all PNSSP showed 10 different patterns.</p><p><strong><em>Conclusion</em>: </strong>In general, the present study suggests that RFLP can be a powerful tool in differentiation between the penicillin resistant and susceptible strains.</p>http://jmb.tums.ac.ir/index.php/jmb/article/view/60HaeІІІ , Pneumococcal,PCR-RFLP, pbp2b |
| spellingShingle | Ali Ahmadi Farzaneh khodaei Maryam Esghaei Gholamreza Irajian Malihe Talebi Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae Journal of Medical Bacteriology HaeІІІ , Pneumococcal,PCR-RFLP, pbp2b |
| title | Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae |
| title_full | Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae |
| title_fullStr | Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae |
| title_full_unstemmed | Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae |
| title_short | Differentiation of Penicillin Susceptible and Nonsusceptible Streptococcus pneumoniae |
| title_sort | differentiation of penicillin susceptible and nonsusceptible streptococcus pneumoniae |
| topic | HaeІІІ , Pneumococcal,PCR-RFLP, pbp2b |
| url | http://jmb.tums.ac.ir/index.php/jmb/article/view/60 |
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