Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish
Polychlorinated biphenyls (PCBs) are widely distributed environmental toxicants, whose biological toxicity is magnified step by step through the transmission of the food chain. However, there is little research about the effect of PCBs on intestinal epithelial barrier function. In this experiment, t...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.929925/full |
| _version_ | 1811225753697648640 |
|---|---|
| author | Di-Hua Zhu Fang-Hong Nie Min Zhang Wan Wei Qing-Lang Song Yao Hu Dan-Ju Kang Zhi-Bao Chen Hong-Ying Lin Jin-Jun Chen |
| author_facet | Di-Hua Zhu Fang-Hong Nie Min Zhang Wan Wei Qing-Lang Song Yao Hu Dan-Ju Kang Zhi-Bao Chen Hong-Ying Lin Jin-Jun Chen |
| author_sort | Di-Hua Zhu |
| collection | DOAJ |
| description | Polychlorinated biphenyls (PCBs) are widely distributed environmental toxicants, whose biological toxicity is magnified step by step through the transmission of the food chain. However, there is little research about the effect of PCBs on intestinal epithelial barrier function. In this experiment, the effects of PCB exposure on the intestines of zebrafish were evaluated. Animals were exposed to Aroclor 1254 (5 μg/L, 10 μg/L, 15 μg/L). After 21 days, the changes in histology, enzyme biomarkers, intestinal microorganisms, and metabolomics were detected. The inflammation and oxidative stress in the intestines of zebrafish were observed. Additionally, there were significant changes in intestinal microbiota and tissue metabolism, most of which were associated with oxidative stress, inflammation, and lipid metabolism. The results showed that PCBs exposure resulted in intestinal inflammation and oxidative stress in zebrafish.Moreover, intestinal metabolites and intestinal microflora of zebrafish were also disturbed. This study verified that exposure can lead to intestinal damage and changes in intestinal metabolic capacity and microorganisms, enlightening the consequences of PCB exposure. |
| first_indexed | 2024-04-12T09:12:45Z |
| format | Article |
| id | doaj.art-826ff354bbb64836ba941d78344514bf |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T09:12:45Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-826ff354bbb64836ba941d78344514bf2022-12-22T03:38:56ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-07-01910.3389/fnut.2022.929925929925Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in ZebrafishDi-Hua Zhu0Fang-Hong Nie1Min Zhang2Wan Wei3Qing-Lang Song4Yao Hu5Dan-Ju Kang6Zhi-Bao Chen7Hong-Ying Lin8Jin-Jun Chen9Department of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaDepartment of Veterinary Medicine, College of Coastal Agriculture, Guangdong Ocean University, Zhanjiang, ChinaPolychlorinated biphenyls (PCBs) are widely distributed environmental toxicants, whose biological toxicity is magnified step by step through the transmission of the food chain. However, there is little research about the effect of PCBs on intestinal epithelial barrier function. In this experiment, the effects of PCB exposure on the intestines of zebrafish were evaluated. Animals were exposed to Aroclor 1254 (5 μg/L, 10 μg/L, 15 μg/L). After 21 days, the changes in histology, enzyme biomarkers, intestinal microorganisms, and metabolomics were detected. The inflammation and oxidative stress in the intestines of zebrafish were observed. Additionally, there were significant changes in intestinal microbiota and tissue metabolism, most of which were associated with oxidative stress, inflammation, and lipid metabolism. The results showed that PCBs exposure resulted in intestinal inflammation and oxidative stress in zebrafish.Moreover, intestinal metabolites and intestinal microflora of zebrafish were also disturbed. This study verified that exposure can lead to intestinal damage and changes in intestinal metabolic capacity and microorganisms, enlightening the consequences of PCB exposure.https://www.frontiersin.org/articles/10.3389/fnut.2022.929925/fullmicrobiomePCBs (polychlorinated biphenyls)gutmetabolomicszebrafish |
| spellingShingle | Di-Hua Zhu Fang-Hong Nie Min Zhang Wan Wei Qing-Lang Song Yao Hu Dan-Ju Kang Zhi-Bao Chen Hong-Ying Lin Jin-Jun Chen Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish Frontiers in Nutrition microbiome PCBs (polychlorinated biphenyls) gut metabolomics zebrafish |
| title | Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish |
| title_full | Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish |
| title_fullStr | Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish |
| title_full_unstemmed | Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish |
| title_short | Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish |
| title_sort | effects of aroclor 1254 on intestinal immunity metabolism and microflora in zebrafish |
| topic | microbiome PCBs (polychlorinated biphenyls) gut metabolomics zebrafish |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.929925/full |
| work_keys_str_mv | AT dihuazhu effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT fanghongnie effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT minzhang effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT wanwei effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT qinglangsong effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT yaohu effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT danjukang effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT zhibaochen effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT hongyinglin effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish AT jinjunchen effectsofaroclor1254onintestinalimmunitymetabolismandmicroflorainzebrafish |