Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress
<i>Casuarina glauca</i> grows in coastal areas suffering long-term damage due to high salt stress. Arbuscular mycorrhizal fungi (AMF) can colonize their roots to alleviate the effects of salt stress. However, the specific molecular mechanism still needs to be further explored. Our physio...
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MDPI AG
2021-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/1/15 |
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| author | Yihan Wang Fengxin Dong Ming Tang |
| author_facet | Yihan Wang Fengxin Dong Ming Tang |
| author_sort | Yihan Wang |
| collection | DOAJ |
| description | <i>Casuarina glauca</i> grows in coastal areas suffering long-term damage due to high salt stress. Arbuscular mycorrhizal fungi (AMF) can colonize their roots to alleviate the effects of salt stress. However, the specific molecular mechanism still needs to be further explored. Our physiological and biochemical analysis showed that <i>Rhizophagus irregularis</i> inoculation played an important role in promoting plant growth, regulating ion balance, and changing the activity of antioxidant enzymes. Transcriptome analysis of roots revealed that 1827 differentially expressed genes (DEGs) were affected by both <i>R. irregularis</i> inoculation and NaCl stress. The enrichment of GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) showed that most of these DEGs were significantly enriched in ion transport, antioxidant enzyme activity, carbohydrate metabolism, and cell wall. <i>HAK5</i>, <i>KAT3</i>, <i>SKOR</i>, <i>PIP1-2</i>, <i>PER64</i>, <i>CPER</i>, <i>GLP10</i>, <i>MYB46</i>, <i>NAC43</i>, <i>WRKY1</i>, and <i>WRKY19</i> were speculated to play the important roles in the salt tolerance of <i>C. glauca</i> induced by <i>R. irregularis</i>. Our research systematically revealed the effect of <i>R. irregularis</i> on the gene expression of <i>C. glauca</i> roots under salt stress, laying a theoretical foundation for the future use of AMF to enhance plant tolerance to salt stress. |
| first_indexed | 2024-03-10T00:54:53Z |
| format | Article |
| id | doaj.art-82704ca9aaac461f8440b4f503d4ab90 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T00:54:53Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-82704ca9aaac461f8440b4f503d4ab902023-11-23T14:45:54ZengMDPI AGMicroorganisms2076-26072021-12-011011510.3390/microorganisms10010015Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl StressYihan Wang0Fengxin Dong1Ming Tang2College of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, ChinaCollege of Forestry, Northwest A&F University, Xianyang 712100, China<i>Casuarina glauca</i> grows in coastal areas suffering long-term damage due to high salt stress. Arbuscular mycorrhizal fungi (AMF) can colonize their roots to alleviate the effects of salt stress. However, the specific molecular mechanism still needs to be further explored. Our physiological and biochemical analysis showed that <i>Rhizophagus irregularis</i> inoculation played an important role in promoting plant growth, regulating ion balance, and changing the activity of antioxidant enzymes. Transcriptome analysis of roots revealed that 1827 differentially expressed genes (DEGs) were affected by both <i>R. irregularis</i> inoculation and NaCl stress. The enrichment of GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) showed that most of these DEGs were significantly enriched in ion transport, antioxidant enzyme activity, carbohydrate metabolism, and cell wall. <i>HAK5</i>, <i>KAT3</i>, <i>SKOR</i>, <i>PIP1-2</i>, <i>PER64</i>, <i>CPER</i>, <i>GLP10</i>, <i>MYB46</i>, <i>NAC43</i>, <i>WRKY1</i>, and <i>WRKY19</i> were speculated to play the important roles in the salt tolerance of <i>C. glauca</i> induced by <i>R. irregularis</i>. Our research systematically revealed the effect of <i>R. irregularis</i> on the gene expression of <i>C. glauca</i> roots under salt stress, laying a theoretical foundation for the future use of AMF to enhance plant tolerance to salt stress.https://www.mdpi.com/2076-2607/10/1/15arbuscular mycorrhizal fungi<i>Casuarina glauca</i>transcriptome analysissalt stress |
| spellingShingle | Yihan Wang Fengxin Dong Ming Tang Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress Microorganisms arbuscular mycorrhizal fungi <i>Casuarina glauca</i> transcriptome analysis salt stress |
| title | Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress |
| title_full | Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress |
| title_fullStr | Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress |
| title_full_unstemmed | Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress |
| title_short | Transcriptome Analysis of Arbuscular Mycorrhizal <i>Casuarina glauca</i> in Damage Mitigation of Roots on NaCl Stress |
| title_sort | transcriptome analysis of arbuscular mycorrhizal i casuarina glauca i in damage mitigation of roots on nacl stress |
| topic | arbuscular mycorrhizal fungi <i>Casuarina glauca</i> transcriptome analysis salt stress |
| url | https://www.mdpi.com/2076-2607/10/1/15 |
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