Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers
<i>Cistus creticus</i> L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog medium (MS), woody plant medium...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/1/104 |
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| author | Kostas Ioannidis Polyxeni Koropouli |
| author_facet | Kostas Ioannidis Polyxeni Koropouli |
| author_sort | Kostas Ioannidis |
| collection | DOAJ |
| description | <i>Cistus creticus</i> L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog medium (MS), woody plant medium (WPM), and Driver and Kuniyaki Walnut medium (DKW) at several strengths (1/8×, 1/4×, 1/2×, 1×, and 2×) on the in vitro growth and organogenesis of rockrose. Significant interactions were observed throughout the experiments between pairs of plant origins, medium types, and strengths, and we also examined the extent to which they affected the studied traits was examined. The types of nutrient medium affected all studied traits except shoot and root percentages. The maximum growth percentage (143.49%) was gained using full-strength WPM. The best performance in shoot percentage was obtained using MS (100%) at several strengths along with 1× WPM (100%). The topmost rooting percentage values (98.61%) were obtained using 1× WPM and 1/2× DKW. The highest number of shoots and roots were observed using full-strength MS (9.39) and half-strength WPM (6.49), respectively. The maximum values for shoot and root length were achieved using 1/2× MS (0.78 cm) and 1/8× WPM (1.55 cm), respectively. The origin of the plant material did not influence any studied trait. Moreover, the genetic relations among the populations used in the in vitro culture were assessed using simple sequence repeats (SSR) markers. Twenty-eight alleles were identified across all five STR loci. The different and effective alleles per locus were 5.60 and 4.72, respectively. The average observed and expected heterozygosity was estimated at 0.52 and 0.72, respectively. Shannon’s information index and the inbreeding coefficient (F) were assessed at 1.48 and 0.30, respectively, revealing a narrow genetic base and high genetic similarity among origins, suggesting that they belong to the same population. |
| first_indexed | 2024-03-08T10:49:21Z |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T10:49:21Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-82708239e4cb43928a81ff153e05f2bf2024-01-26T16:50:00ZengMDPI AGHorticulturae2311-75242024-01-0110110410.3390/horticulturae10010104Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular MarkersKostas Ioannidis0Polyxeni Koropouli1Laboratory of Forest Genetics and Biotechnology, Institute of Mediterranean and Forest Ecosystems, Hellenic Agricultural Organization “Demeter”, Ilissia, 11528 Athens, GreeceMinistry of Education, 15180 Athens, Greece<i>Cistus creticus</i> L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog medium (MS), woody plant medium (WPM), and Driver and Kuniyaki Walnut medium (DKW) at several strengths (1/8×, 1/4×, 1/2×, 1×, and 2×) on the in vitro growth and organogenesis of rockrose. Significant interactions were observed throughout the experiments between pairs of plant origins, medium types, and strengths, and we also examined the extent to which they affected the studied traits was examined. The types of nutrient medium affected all studied traits except shoot and root percentages. The maximum growth percentage (143.49%) was gained using full-strength WPM. The best performance in shoot percentage was obtained using MS (100%) at several strengths along with 1× WPM (100%). The topmost rooting percentage values (98.61%) were obtained using 1× WPM and 1/2× DKW. The highest number of shoots and roots were observed using full-strength MS (9.39) and half-strength WPM (6.49), respectively. The maximum values for shoot and root length were achieved using 1/2× MS (0.78 cm) and 1/8× WPM (1.55 cm), respectively. The origin of the plant material did not influence any studied trait. Moreover, the genetic relations among the populations used in the in vitro culture were assessed using simple sequence repeats (SSR) markers. Twenty-eight alleles were identified across all five STR loci. The different and effective alleles per locus were 5.60 and 4.72, respectively. The average observed and expected heterozygosity was estimated at 0.52 and 0.72, respectively. Shannon’s information index and the inbreeding coefficient (F) were assessed at 1.48 and 0.30, respectively, revealing a narrow genetic base and high genetic similarity among origins, suggesting that they belong to the same population.https://www.mdpi.com/2311-7524/10/1/104rockroseMSWPMDKWin vitro micropropagationshoot induction |
| spellingShingle | Kostas Ioannidis Polyxeni Koropouli Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers Horticulturae rockrose MS WPM DKW in vitro micropropagation shoot induction |
| title | Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers |
| title_full | Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers |
| title_fullStr | Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers |
| title_full_unstemmed | Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers |
| title_short | Effects of Different Media and Their Strengths in In Vitro Culture of Three Different <i>Cistus creticus</i> L. Populations and Their Genetic Assessment Using Simple Sequence Repeat Molecular Markers |
| title_sort | effects of different media and their strengths in in vitro culture of three different i cistus creticus i l populations and their genetic assessment using simple sequence repeat molecular markers |
| topic | rockrose MS WPM DKW in vitro micropropagation shoot induction |
| url | https://www.mdpi.com/2311-7524/10/1/104 |
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