Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet
IntroductionAs a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1051200/full |
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| author | Guangwen Yan Shuaibing Li Shuaibing Li Yuhang Wen Yuhang Wen Yadan Luo Yadan Luo Jingrong Huang Jingrong Huang Baoting Chen Baoting Chen Shuya Lv Shuya Lv Lang Chen Lang Chen Lvqin He Lvqin He Manli He Manli He Qian Yang Qian Yang Zehui Yu Zehui Yu Wudian Xiao Wudian Xiao Yong Tang Yong Tang Weiyao Li Jianhong Han Jianhong Han Fangfang Zhao Shumin Yu Fang Kong Fang Kong Benazir Abbasi Hongmei Yin Congwei Gu Congwei Gu Congwei Gu |
| author_facet | Guangwen Yan Shuaibing Li Shuaibing Li Yuhang Wen Yuhang Wen Yadan Luo Yadan Luo Jingrong Huang Jingrong Huang Baoting Chen Baoting Chen Shuya Lv Shuya Lv Lang Chen Lang Chen Lvqin He Lvqin He Manli He Manli He Qian Yang Qian Yang Zehui Yu Zehui Yu Wudian Xiao Wudian Xiao Yong Tang Yong Tang Weiyao Li Jianhong Han Jianhong Han Fangfang Zhao Shumin Yu Fang Kong Fang Kong Benazir Abbasi Hongmei Yin Congwei Gu Congwei Gu Congwei Gu |
| author_sort | Guangwen Yan |
| collection | DOAJ |
| description | IntroductionAs a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.MethodsThe NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon (formed fecal balls) were collected for 16S rRNA gene analysis.ResultsCompared with normal mice, the diversity and the relative abundance of major bacteria and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. In NAFLD mice, the variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different.DiscussionTherefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota. |
| first_indexed | 2024-04-11T05:40:11Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T05:40:11Z |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-8270f192bdd943578ca53a782fc9ac732022-12-22T04:59:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10512001051200Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat dietGuangwen Yan0Shuaibing Li1Shuaibing Li2Yuhang Wen3Yuhang Wen4Yadan Luo5Yadan Luo6Jingrong Huang7Jingrong Huang8Baoting Chen9Baoting Chen10Shuya Lv11Shuya Lv12Lang Chen13Lang Chen14Lvqin He15Lvqin He16Manli He17Manli He18Qian Yang19Qian Yang20Zehui Yu21Zehui Yu22Wudian Xiao23Wudian Xiao24Yong Tang25Yong Tang26Weiyao Li27Jianhong Han28Jianhong Han29Fangfang Zhao30Shumin Yu31Fang Kong32Fang Kong33Benazir Abbasi34Hongmei Yin35Congwei Gu36Congwei Gu37Congwei Gu38College of Animal Science, Xichang University, Xichang, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao SAR, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, ChinaCollege of Animal Science, Xichang University, Xichang, ChinaLaboratory Animal Centre, Southwest Medical University, Luzhou, ChinaModel Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaIntroductionAs a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.MethodsThe NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon (formed fecal balls) were collected for 16S rRNA gene analysis.ResultsCompared with normal mice, the diversity and the relative abundance of major bacteria and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. In NAFLD mice, the variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different.DiscussionTherefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1051200/fullnon-alcoholic fatty liver diseaseintestinal microbiota16S rDNA sequencingmicehigh-fat diet |
| spellingShingle | Guangwen Yan Shuaibing Li Shuaibing Li Yuhang Wen Yuhang Wen Yadan Luo Yadan Luo Jingrong Huang Jingrong Huang Baoting Chen Baoting Chen Shuya Lv Shuya Lv Lang Chen Lang Chen Lvqin He Lvqin He Manli He Manli He Qian Yang Qian Yang Zehui Yu Zehui Yu Wudian Xiao Wudian Xiao Yong Tang Yong Tang Weiyao Li Jianhong Han Jianhong Han Fangfang Zhao Shumin Yu Fang Kong Fang Kong Benazir Abbasi Hongmei Yin Congwei Gu Congwei Gu Congwei Gu Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet Frontiers in Microbiology non-alcoholic fatty liver disease intestinal microbiota 16S rDNA sequencing mice high-fat diet |
| title | Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet |
| title_full | Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet |
| title_fullStr | Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet |
| title_full_unstemmed | Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet |
| title_short | Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet |
| title_sort | characteristics of intestinal microbiota in c57bl 6 mice with non alcoholic fatty liver induced by high fat diet |
| topic | non-alcoholic fatty liver disease intestinal microbiota 16S rDNA sequencing mice high-fat diet |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1051200/full |
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