Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity
This paper evaluates CHCl3 and CH3OH extracts of the stem bark, branches and leaves of Drimys brasiliensis and drimane sesquiterpenes isolated from the stem bark against strains of Leishmania amazonensis and Leishmania braziliensis promastigotes and Plasmodium falciparum trophozoites. All of the ext...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2013-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200140&lng=en&tlng=en |
| _version_ | 1797718232246779904 |
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| author | Vanessa Duarte Claudino Kesia Caroline da Silva Valdir Cechinel Filho Rosendo Augusto Yunes Franco Delle Monache Alberto Giménez Efrain Salamanca David Gutierrez-Yapu Angela Malheiros |
| author_facet | Vanessa Duarte Claudino Kesia Caroline da Silva Valdir Cechinel Filho Rosendo Augusto Yunes Franco Delle Monache Alberto Giménez Efrain Salamanca David Gutierrez-Yapu Angela Malheiros |
| author_sort | Vanessa Duarte Claudino |
| collection | DOAJ |
| description | This paper evaluates CHCl3 and CH3OH extracts of the stem bark, branches and leaves of Drimys brasiliensis and drimane sesquiterpenes isolated from the stem bark against strains of Leishmania amazonensis and Leishmania braziliensis promastigotes and Plasmodium falciparum trophozoites. All of the extracts and compounds were tested in cell lines in comparison with reference standards and cell viability was determined by the XTT method. The CHCl3 and CH3OH extracts from the stem bark and branches yielded promising results against two strains of Leishmania, with 50% inhibitory concentrations (IC50 ) values ranging from 39-100 µg/mL. The CHCl3 extract of the stem bark returned IC50 values of 39 and 40.6 µg/mL for L. amazonensis and L. braziliensis, respectively. The drimanes were relatively effective: 1-β-(p-coumaroyloxy)-polygodial produced IC50 values of 5.55 and 2.52 µM for L. amazonensis and L. braziliensis, respectively, compared with 1-β-(p-methoxycinnamoyl)-polygodial, which produced respective IC50 values of 15.85 and 17.80 µM. The CHCl3 extract demonstrated activity (IC50 of 3.0 µg/mL) against P. falciparum. The IC50 values of 1-β-(p-cumaroyloxyl)-polygodial and 1-β-(p-methoxycinnamoyl)-polygodial were 1.01 and 4.87 µM, respectively, for the trophozoite strain. Therefore, the results suggest that D. brasiliensis is a promising plant from which to obtain new and effective antiparasitic agents. |
| first_indexed | 2024-03-12T08:47:53Z |
| format | Article |
| id | doaj.art-8271cd13e6d5403fb93051a886bc4baf |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:47:53Z |
| publishDate | 2013-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-8271cd13e6d5403fb93051a886bc4baf2023-09-02T16:25:07ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602013-04-01108214014410.1590/0074-0276108022013002S0074-02762013000200140Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activityVanessa Duarte ClaudinoKesia Caroline da SilvaValdir Cechinel FilhoRosendo Augusto YunesFranco Delle MonacheAlberto GiménezEfrain SalamancaDavid Gutierrez-YapuAngela MalheirosThis paper evaluates CHCl3 and CH3OH extracts of the stem bark, branches and leaves of Drimys brasiliensis and drimane sesquiterpenes isolated from the stem bark against strains of Leishmania amazonensis and Leishmania braziliensis promastigotes and Plasmodium falciparum trophozoites. All of the extracts and compounds were tested in cell lines in comparison with reference standards and cell viability was determined by the XTT method. The CHCl3 and CH3OH extracts from the stem bark and branches yielded promising results against two strains of Leishmania, with 50% inhibitory concentrations (IC50 ) values ranging from 39-100 µg/mL. The CHCl3 extract of the stem bark returned IC50 values of 39 and 40.6 µg/mL for L. amazonensis and L. braziliensis, respectively. The drimanes were relatively effective: 1-β-(p-coumaroyloxy)-polygodial produced IC50 values of 5.55 and 2.52 µM for L. amazonensis and L. braziliensis, respectively, compared with 1-β-(p-methoxycinnamoyl)-polygodial, which produced respective IC50 values of 15.85 and 17.80 µM. The CHCl3 extract demonstrated activity (IC50 of 3.0 µg/mL) against P. falciparum. The IC50 values of 1-β-(p-cumaroyloxyl)-polygodial and 1-β-(p-methoxycinnamoyl)-polygodial were 1.01 and 4.87 µM, respectively, for the trophozoite strain. Therefore, the results suggest that D. brasiliensis is a promising plant from which to obtain new and effective antiparasitic agents.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200140&lng=en&tlng=enleishmaniasismalariaDrimysdrimane sesquiterpenes |
| spellingShingle | Vanessa Duarte Claudino Kesia Caroline da Silva Valdir Cechinel Filho Rosendo Augusto Yunes Franco Delle Monache Alberto Giménez Efrain Salamanca David Gutierrez-Yapu Angela Malheiros Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity Memorias do Instituto Oswaldo Cruz leishmaniasis malaria Drimys drimane sesquiterpenes |
| title | Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity |
| title_full | Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity |
| title_fullStr | Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity |
| title_full_unstemmed | Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity |
| title_short | Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity |
| title_sort | drimanes from drimys brasiliensis with leishmanicidal and antimalarial activity |
| topic | leishmaniasis malaria Drimys drimane sesquiterpenes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200140&lng=en&tlng=en |
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