Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene
A novel tobacco(nicotiana tabacum) sucrose synthase genewas characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. Thissucrose synthase gene mRNA was 2,954bp in length containing a 2,418bp open reading framewhich encodes a protein of 805 amino acids. Sequence anal...
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| Format: | Article |
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EDP Sciences
2021-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/68/e3sconf_netid21_03101.pdf |
| _version_ | 1819240063800705024 |
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| author | Zhao Meiwei Zou Juan Leng Sixuan Tao Ying Zhao Zhengxiong |
| author_facet | Zhao Meiwei Zou Juan Leng Sixuan Tao Ying Zhao Zhengxiong |
| author_sort | Zhao Meiwei |
| collection | DOAJ |
| description | A novel tobacco(nicotiana tabacum) sucrose synthase genewas characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. Thissucrose synthase gene mRNA was 2,954bp in length containing a 2,418bp open reading framewhich encodes a protein of 805 amino acids. Sequence analysis revealed that thissucrose synthase of tobacco shares high homology with the sucrose synthase of Nicotianaattenuata(98%), Nicotianasylvestris(98%), Nicotianatomentosiformis(98%), Capsicumannuum(95%), Capsicumbaccatum(95%), Solanumtuberosum(94%), Solanumlycopersicum(94%) and Solanumpennellii(94%). Results also showed that tobacco sucrose synthase gene has a closer genetic relationship with the sucrose synthase gene of Capsicumannuum.Genomic DNA analysis indicated that tobacco sucrose synthase gene is structured in 13 exons and 12 introns.Tissue expression profile analysis revealed that this tobacco sucrose synthase gene was highly expressed in leaf, but moderately expressed in root, stemand flower. These established the foundation for further research on the tobacco sucrose synthase gene. |
| first_indexed | 2024-12-23T14:02:04Z |
| format | Article |
| id | doaj.art-827229b466c444788f2cfb5c7bcec499 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-23T14:02:04Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-827229b466c444788f2cfb5c7bcec4992022-12-21T17:44:18ZengEDP SciencesE3S Web of Conferences2267-12422021-01-012920310110.1051/e3sconf/202129203101e3sconf_netid21_03101Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase GeneZhao Meiwei0Zou Juan1Leng Sixuan2Tao Ying3Zhao Zhengxiong4College of Agronomy and Biotechnology, Yunnan Agricultural UniversityResearch and Development Center, China Tobacco Yunnan Industrial Co.,LtdResearch and Development Center, China Tobacco Yunnan Industrial Co.,LtdResearch and Development Center, China Tobacco Yunnan Industrial Co.,LtdCollege of Resources and Environment, Yunnan Agricultural UniversityA novel tobacco(nicotiana tabacum) sucrose synthase genewas characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. Thissucrose synthase gene mRNA was 2,954bp in length containing a 2,418bp open reading framewhich encodes a protein of 805 amino acids. Sequence analysis revealed that thissucrose synthase of tobacco shares high homology with the sucrose synthase of Nicotianaattenuata(98%), Nicotianasylvestris(98%), Nicotianatomentosiformis(98%), Capsicumannuum(95%), Capsicumbaccatum(95%), Solanumtuberosum(94%), Solanumlycopersicum(94%) and Solanumpennellii(94%). Results also showed that tobacco sucrose synthase gene has a closer genetic relationship with the sucrose synthase gene of Capsicumannuum.Genomic DNA analysis indicated that tobacco sucrose synthase gene is structured in 13 exons and 12 introns.Tissue expression profile analysis revealed that this tobacco sucrose synthase gene was highly expressed in leaf, but moderately expressed in root, stemand flower. These established the foundation for further research on the tobacco sucrose synthase gene.https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/68/e3sconf_netid21_03101.pdf |
| spellingShingle | Zhao Meiwei Zou Juan Leng Sixuan Tao Ying Zhao Zhengxiong Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene E3S Web of Conferences |
| title | Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene |
| title_full | Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene |
| title_fullStr | Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene |
| title_full_unstemmed | Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene |
| title_short | Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) Sucrose Synthase Gene |
| title_sort | initial characterization and expression pattern analysis of tobacco nicotiana tabacum sucrose synthase gene |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/68/e3sconf_netid21_03101.pdf |
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