Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue
Laser capture microdissection (LCM) coupled to label-free quantitative mass spectrometry is a viable strategy to identify biomarkers from infected tissues. In this study, LCM was employed to take a “snapshot” of proteins produced in vivo during <i>Coccidiodies</i> spp. infection in human...
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MDPI AG
2020-12-01
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Series: | Journal of Fungi |
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Online Access: | https://www.mdpi.com/2309-608X/6/4/365 |
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author | Natalie M. Mitchell Surendra Dasari Thomas E. Grys Douglas F. Lake |
author_facet | Natalie M. Mitchell Surendra Dasari Thomas E. Grys Douglas F. Lake |
author_sort | Natalie M. Mitchell |
collection | DOAJ |
description | Laser capture microdissection (LCM) coupled to label-free quantitative mass spectrometry is a viable strategy to identify biomarkers from infected tissues. In this study, LCM was employed to take a “snapshot” of proteins produced in vivo during <i>Coccidiodies</i> spp. infection in human lungs. Proteomic analysis of LCM lung sections revealed hundreds of hosts and Coccidioidal proteins. Twenty-seven highly abundant <i>Coccidioides</i> spp. proteins were identified which do not share significant sequence orthology with human proteins. Three of the 27 Coccidioidal proteins are also potential <i>Coccidoides</i>-specific biomarkers, as they also do not share sequence homology to any other pathogenic fungus or microbe. Gene ontology analysis of the 27 biomarker candidate proteins revealed enriched hydrolase activity and increased purine and carbohydrate metabolism functions. Finally, we provide proteomic evidence that all 27 biomarker candidates are produced by the fungus when grown in vitro in a media- and growth-phase dependent manner. |
first_indexed | 2024-03-10T14:04:58Z |
format | Article |
id | doaj.art-82724563af5348e1b09d78e6d2377565 |
institution | Directory Open Access Journal |
issn | 2309-608X |
language | English |
last_indexed | 2024-03-10T14:04:58Z |
publishDate | 2020-12-01 |
publisher | MDPI AG |
record_format | Article |
series | Journal of Fungi |
spelling | doaj.art-82724563af5348e1b09d78e6d23775652023-11-21T00:47:13ZengMDPI AGJournal of Fungi2309-608X2020-12-016436510.3390/jof6040365Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung TissueNatalie M. Mitchell0Surendra Dasari1Thomas E. Grys2Douglas F. Lake3School of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ 85259, USADepartment of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, MN 55905, USADepartment of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ 85054, USASchool of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ 85259, USALaser capture microdissection (LCM) coupled to label-free quantitative mass spectrometry is a viable strategy to identify biomarkers from infected tissues. In this study, LCM was employed to take a “snapshot” of proteins produced in vivo during <i>Coccidiodies</i> spp. infection in human lungs. Proteomic analysis of LCM lung sections revealed hundreds of hosts and Coccidioidal proteins. Twenty-seven highly abundant <i>Coccidioides</i> spp. proteins were identified which do not share significant sequence orthology with human proteins. Three of the 27 Coccidioidal proteins are also potential <i>Coccidoides</i>-specific biomarkers, as they also do not share sequence homology to any other pathogenic fungus or microbe. Gene ontology analysis of the 27 biomarker candidate proteins revealed enriched hydrolase activity and increased purine and carbohydrate metabolism functions. Finally, we provide proteomic evidence that all 27 biomarker candidates are produced by the fungus when grown in vitro in a media- and growth-phase dependent manner.https://www.mdpi.com/2309-608X/6/4/365<i>Coccidioides</i>Valley Feverbiomarkermass spectrometrylaser capture microdissection |
spellingShingle | Natalie M. Mitchell Surendra Dasari Thomas E. Grys Douglas F. Lake Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue Journal of Fungi <i>Coccidioides</i> Valley Fever biomarker mass spectrometry laser capture microdissection |
title | Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue |
title_full | Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue |
title_fullStr | Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue |
title_full_unstemmed | Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue |
title_short | Laser Capture Microdissection-Assisted Protein Biomarker Discovery from <i>Coccidioides</i>-Infected Lung Tissue |
title_sort | laser capture microdissection assisted protein biomarker discovery from i coccidioides i infected lung tissue |
topic | <i>Coccidioides</i> Valley Fever biomarker mass spectrometry laser capture microdissection |
url | https://www.mdpi.com/2309-608X/6/4/365 |
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