Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress
Plants are markedly affected by drought stress caused by fluctuations in global climate, reduction in rainfall and a decrease in soil fertility. Therefore, some mechanistic strategies to cope with adverse effects of drought stress are needed. Alpha lipoic acid (ALA), a potent antioxidant molecule, i...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2018-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641800011T.pdf |
| _version_ | 1811324065600765952 |
|---|---|
| author | Terzi Rabiye Saruhan Güler Neslihan Güven Funda Gül Kadioglu Asim |
| author_facet | Terzi Rabiye Saruhan Güler Neslihan Güven Funda Gül Kadioglu Asim |
| author_sort | Terzi Rabiye |
| collection | DOAJ |
| description | Plants are markedly affected by drought stress caused by fluctuations in global climate, reduction in rainfall and a decrease in soil fertility. Therefore, some mechanistic strategies to cope with adverse effects of drought stress are needed. Alpha lipoic acid (ALA), a potent antioxidant molecule, is known to function in abiotic stress tolerance. In the current study, we investigated the ALA-stimulated physiological role in tolerance to osmotic stress induced by polyethylene glycol in two maize (Zea mays L.) cultivars (cv. Helen and cv. Akpinar). Application of ALA increased the leaf water potential of maize cultivars under stressful and stress-free conditions but decreased lipid peroxidation and the hydrogen peroxide (H2O2) content. Additionally, enhanced activity of the antioxidant defense system was observed following ALA application. Exogenous ALA elevated the activities of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) under osmotic stress as compared to seedlings not exposed to ALA. Conversely, ascorbate peroxidase (APX) activity was decreased by ALA application in both cultivars. Higher GR and MDHAR activities of both cultivars were simultaneously observed in ALA treatments under osmotic stress. Taken together, the data indicated that exogenous ALA may function in arranging resilience against osmotic stress by reducing oxidative damage through induction of the antioxidant machinery in maize cultivars. |
| first_indexed | 2024-04-13T14:07:38Z |
| format | Article |
| id | doaj.art-827a3628c30c4028a0b80a633ae3f443 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-13T14:07:38Z |
| publishDate | 2018-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-827a3628c30c4028a0b80a633ae3f4432022-12-22T02:43:52ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392018-01-0170350351110.2298/ABS171218011T0354-46641800011TAlpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stressTerzi Rabiye0Saruhan Güler Neslihan1Güven Funda Gül2Kadioglu Asim3Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, TurkeyDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, Trabzon, TurkeyDepartment of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, TurkeyDepartment of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, TurkeyPlants are markedly affected by drought stress caused by fluctuations in global climate, reduction in rainfall and a decrease in soil fertility. Therefore, some mechanistic strategies to cope with adverse effects of drought stress are needed. Alpha lipoic acid (ALA), a potent antioxidant molecule, is known to function in abiotic stress tolerance. In the current study, we investigated the ALA-stimulated physiological role in tolerance to osmotic stress induced by polyethylene glycol in two maize (Zea mays L.) cultivars (cv. Helen and cv. Akpinar). Application of ALA increased the leaf water potential of maize cultivars under stressful and stress-free conditions but decreased lipid peroxidation and the hydrogen peroxide (H2O2) content. Additionally, enhanced activity of the antioxidant defense system was observed following ALA application. Exogenous ALA elevated the activities of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) under osmotic stress as compared to seedlings not exposed to ALA. Conversely, ascorbate peroxidase (APX) activity was decreased by ALA application in both cultivars. Higher GR and MDHAR activities of both cultivars were simultaneously observed in ALA treatments under osmotic stress. Taken together, the data indicated that exogenous ALA may function in arranging resilience against osmotic stress by reducing oxidative damage through induction of the antioxidant machinery in maize cultivars.http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641800011T.pdfalpha lipoic acidantioxidant systemosmotic stresstoleranceZea mays |
| spellingShingle | Terzi Rabiye Saruhan Güler Neslihan Güven Funda Gül Kadioglu Asim Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress Archives of Biological Sciences alpha lipoic acid antioxidant system osmotic stress tolerance Zea mays |
| title | Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress |
| title_full | Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress |
| title_fullStr | Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress |
| title_full_unstemmed | Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress |
| title_short | Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress |
| title_sort | alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress |
| topic | alpha lipoic acid antioxidant system osmotic stress tolerance Zea mays |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641800011T.pdf |
| work_keys_str_mv | AT terzirabiye alphalipoicacidtreatmentinducestheantioxidantsystemandameliorateslipidperoxidationinmaizeseedlingsunderosmoticstress AT saruhangulerneslihan alphalipoicacidtreatmentinducestheantioxidantsystemandameliorateslipidperoxidationinmaizeseedlingsunderosmoticstress AT guvenfundagul alphalipoicacidtreatmentinducestheantioxidantsystemandameliorateslipidperoxidationinmaizeseedlingsunderosmoticstress AT kadiogluasim alphalipoicacidtreatmentinducestheantioxidantsystemandameliorateslipidperoxidationinmaizeseedlingsunderosmoticstress |