The sickle cell trait and end stage renal disease in Salvador, Brazil.
BACKGROUND:Carriers of the sickle cell trait (HbAS) usually remain asymptomatic. However, under conditions of low tissue oxygenation, red blood cell sickling and vascular obstruction may develop. Chronic kidney disease (CKD) can arise from conditions promoting low-oxygen in kidney tissue, which may...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0209036 |
| _version_ | 1819260186953515008 |
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| author | Dona J Alladagbin Paula N Fernandes Maria B Tavares Jean T Brito Geraldo G S Oliveira Luciano K Silva Nadia A Khouri Marilia B Oliveira Tatiana Amorim Cácia M Matos Guilherme S Ribeiro Antônio A Lopes Marilda S Gonçalves Washington L C Dos-Santos |
| author_facet | Dona J Alladagbin Paula N Fernandes Maria B Tavares Jean T Brito Geraldo G S Oliveira Luciano K Silva Nadia A Khouri Marilia B Oliveira Tatiana Amorim Cácia M Matos Guilherme S Ribeiro Antônio A Lopes Marilda S Gonçalves Washington L C Dos-Santos |
| author_sort | Dona J Alladagbin |
| collection | DOAJ |
| description | BACKGROUND:Carriers of the sickle cell trait (HbAS) usually remain asymptomatic. However, under conditions of low tissue oxygenation, red blood cell sickling and vascular obstruction may develop. Chronic kidney disease (CKD) can arise from conditions promoting low-oxygen in kidney tissue, which may be aggravated by the presence of the sickle cell trait. In addition, CKD can arise from other genetic traits. AIM:To compare the frequency of HbAS among hemodialysis patients and the general newborn population of Salvador (Bahia-Brazil), as well as to investigate the frequencies of apolipoprotein L1 risk variants in patients undergoing hemodialysis. METHODS:A cross-sectional study included 306 patients with ESRD (End Stage Renal Disease) on hemodialysis for no more than three years. Hemoglobin profiles were characterized by high-performance liquid chromatography. To estimate the sickle cell trait frequency in the general population of Salvador, we analyzed data collected by a local neonatal screening program between 2011 and 2016. To exclude the potential contributing effect of the apolipoprotein L1 (APOL1) gene variants, we performed genotyping by PCR and DNA sequencing of 45 patients. RESULTS:The frequency of HbAS was significantly higher in hemodialysis patients (9.8%) than in the general population (4.6%): Odds Ratio = 2.32 (95% CI = 1.59-3.38). No differences in demographic, clinical or laboratory data were found among patients with or without the sickle cell trait. The frequency of patients with none, one or two APOL1 risk haplotypes (G1 and G2) for CKD were 80%, 18% and 2%, respectively. CONCLUSIONS:The frequency of the sickle cell trait is higher in patients with ESRD on hemodialysis compared to the general population. APOL1 haplotypes do not seem to be the determinant of ESRD in these patients. |
| first_indexed | 2024-12-23T19:21:55Z |
| format | Article |
| id | doaj.art-827ac5349c7a4824b3cc94e8e74eeed1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:21:55Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-827ac5349c7a4824b3cc94e8e74eeed12022-12-21T17:34:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011312e020903610.1371/journal.pone.0209036The sickle cell trait and end stage renal disease in Salvador, Brazil.Dona J AlladagbinPaula N FernandesMaria B TavaresJean T BritoGeraldo G S OliveiraLuciano K SilvaNadia A KhouriMarilia B OliveiraTatiana AmorimCácia M MatosGuilherme S RibeiroAntônio A LopesMarilda S GonçalvesWashington L C Dos-SantosBACKGROUND:Carriers of the sickle cell trait (HbAS) usually remain asymptomatic. However, under conditions of low tissue oxygenation, red blood cell sickling and vascular obstruction may develop. Chronic kidney disease (CKD) can arise from conditions promoting low-oxygen in kidney tissue, which may be aggravated by the presence of the sickle cell trait. In addition, CKD can arise from other genetic traits. AIM:To compare the frequency of HbAS among hemodialysis patients and the general newborn population of Salvador (Bahia-Brazil), as well as to investigate the frequencies of apolipoprotein L1 risk variants in patients undergoing hemodialysis. METHODS:A cross-sectional study included 306 patients with ESRD (End Stage Renal Disease) on hemodialysis for no more than three years. Hemoglobin profiles were characterized by high-performance liquid chromatography. To estimate the sickle cell trait frequency in the general population of Salvador, we analyzed data collected by a local neonatal screening program between 2011 and 2016. To exclude the potential contributing effect of the apolipoprotein L1 (APOL1) gene variants, we performed genotyping by PCR and DNA sequencing of 45 patients. RESULTS:The frequency of HbAS was significantly higher in hemodialysis patients (9.8%) than in the general population (4.6%): Odds Ratio = 2.32 (95% CI = 1.59-3.38). No differences in demographic, clinical or laboratory data were found among patients with or without the sickle cell trait. The frequency of patients with none, one or two APOL1 risk haplotypes (G1 and G2) for CKD were 80%, 18% and 2%, respectively. CONCLUSIONS:The frequency of the sickle cell trait is higher in patients with ESRD on hemodialysis compared to the general population. APOL1 haplotypes do not seem to be the determinant of ESRD in these patients.https://doi.org/10.1371/journal.pone.0209036 |
| spellingShingle | Dona J Alladagbin Paula N Fernandes Maria B Tavares Jean T Brito Geraldo G S Oliveira Luciano K Silva Nadia A Khouri Marilia B Oliveira Tatiana Amorim Cácia M Matos Guilherme S Ribeiro Antônio A Lopes Marilda S Gonçalves Washington L C Dos-Santos The sickle cell trait and end stage renal disease in Salvador, Brazil. PLoS ONE |
| title | The sickle cell trait and end stage renal disease in Salvador, Brazil. |
| title_full | The sickle cell trait and end stage renal disease in Salvador, Brazil. |
| title_fullStr | The sickle cell trait and end stage renal disease in Salvador, Brazil. |
| title_full_unstemmed | The sickle cell trait and end stage renal disease in Salvador, Brazil. |
| title_short | The sickle cell trait and end stage renal disease in Salvador, Brazil. |
| title_sort | sickle cell trait and end stage renal disease in salvador brazil |
| url | https://doi.org/10.1371/journal.pone.0209036 |
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