| Summary: | Petroleum-derived substances (PDSs) as main pollutants of the natural environment can negatively affect the microbiological, biochemical, and biological properties of agricultural soils and, consequently, plant production. The present study aimed to determine the after-effect of PDSs such as petrol, used engine oil, and diesel fuel on the activity of selected soil enzymes (phosphatase, dehydrogenase, and urease) and on the occurrence of soil invertebrates. Moreover, changes in the analyzed parameters in response to bioremediation of the polluted soil by using ZB-01 preparation were investigated. The field experiments were performed four- and five-years post contamination. The results showed that even after five years, PDSs significantly modified the activity of soil enzymes; however, this effect was often varied, depending on the pollutant, enzyme, and time after soil contamination. Dehydrogenase seems to be a good indicator of soil contamination with PDSs, particularly diesel fuel. Engine oil and diesel fuel limited still the occurrence of soil invertebrates, particularly Collembola from the families Hypogastruidae, Isotomidae, and Entomobryidae, even after four and five years of contamination. This finding suggests the usefulness of these organisms in assessing soil pollution and in monitoring the progress of bioremediation. The effect of ZB-01 biopreparation on the activity of selected enzymes was varied. Its effect on the occurrence of soil invertebrates was usually beneficial, which was evident in diesel fuel-contaminated soil.
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