Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
Brazil has experienced several arbovirus outbreaks in recent years, among which yellow fever stands out. The state of Minas Gerais faced outbreaks of sylvatic yellow fever in 2017 and 2018, with 1002 confirmed cases and 340 deaths. This work presents the results of survey efforts to detect the yello...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/10/5/136 |
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| author | Guilherme Garcia Pinheiro Marcele Neves Rocha Maria Angélica de Oliveira Luciano Andrade Moreira José Dilermando Andrade Filho |
| author_facet | Guilherme Garcia Pinheiro Marcele Neves Rocha Maria Angélica de Oliveira Luciano Andrade Moreira José Dilermando Andrade Filho |
| author_sort | Guilherme Garcia Pinheiro |
| collection | DOAJ |
| description | Brazil has experienced several arbovirus outbreaks in recent years, among which yellow fever stands out. The state of Minas Gerais faced outbreaks of sylvatic yellow fever in 2017 and 2018, with 1002 confirmed cases and 340 deaths. This work presents the results of survey efforts to detect the yellow fever virus in mosquitoes from two conservation areas in the metropolitan region of Belo Horizonte, Brazil. A total of 867 mosquitoes of 20 species were collected between September 2017 and May 2018, the most abundant being <i>Psorophora</i> (<i>Janthinosoma</i>) <i>ferox</i> (von Humboldt, 1819) (31.3%), <i>Limatus durhamii</i> Theobald, 1901 (19.1%) and <i>Haemagogus</i> (<i>Haemagogus</i>) <i>janthinomys</i> Dyar, 1921 (18.2%). Total RNA was extracted from the mosquitoes for real-time PCR analysis for yellow fever, chikungunya, mayaro, Zika and dengue viruses. The yellow fever infection rate was 8.2% for <i>Hg. janthinomys</i> (13 mosquitoes), which is the main vector of sylvatic yellow fever in Brazil. In addition to surveying the mosquito fauna of these conservation units, this work demonstrates the importance of monitoring the circulation of viruses near large urban centers. |
| first_indexed | 2024-04-12T20:54:49Z |
| format | Article |
| id | doaj.art-827da0b15377486092a67e851b5aa698 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-04-12T20:54:49Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-827da0b15377486092a67e851b5aa6982022-12-22T03:17:01ZengMDPI AGInsects2075-44502019-05-0110513610.3390/insects10050136insects10050136Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, BrazilGuilherme Garcia Pinheiro0Marcele Neves Rocha1Maria Angélica de Oliveira2Luciano Andrade Moreira3José Dilermando Andrade Filho4Coleção de Mosquitos Neotropicais, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, BrazilMosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, BrazilColeção de Mosquitos Neotropicais, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, BrazilMosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, BrazilGrupo de Estudos em Leishmanioses, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, BrazilBrazil has experienced several arbovirus outbreaks in recent years, among which yellow fever stands out. The state of Minas Gerais faced outbreaks of sylvatic yellow fever in 2017 and 2018, with 1002 confirmed cases and 340 deaths. This work presents the results of survey efforts to detect the yellow fever virus in mosquitoes from two conservation areas in the metropolitan region of Belo Horizonte, Brazil. A total of 867 mosquitoes of 20 species were collected between September 2017 and May 2018, the most abundant being <i>Psorophora</i> (<i>Janthinosoma</i>) <i>ferox</i> (von Humboldt, 1819) (31.3%), <i>Limatus durhamii</i> Theobald, 1901 (19.1%) and <i>Haemagogus</i> (<i>Haemagogus</i>) <i>janthinomys</i> Dyar, 1921 (18.2%). Total RNA was extracted from the mosquitoes for real-time PCR analysis for yellow fever, chikungunya, mayaro, Zika and dengue viruses. The yellow fever infection rate was 8.2% for <i>Hg. janthinomys</i> (13 mosquitoes), which is the main vector of sylvatic yellow fever in Brazil. In addition to surveying the mosquito fauna of these conservation units, this work demonstrates the importance of monitoring the circulation of viruses near large urban centers.https://www.mdpi.com/2075-4450/10/5/136arbovirusesyellow fevermosquitoes |
| spellingShingle | Guilherme Garcia Pinheiro Marcele Neves Rocha Maria Angélica de Oliveira Luciano Andrade Moreira José Dilermando Andrade Filho Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil Insects arboviruses yellow fever mosquitoes |
| title | Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil |
| title_full | Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil |
| title_fullStr | Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil |
| title_full_unstemmed | Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil |
| title_short | Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil |
| title_sort | detection of yellow fever virus in sylvatic mosquitoes during disease outbreaks of 2017 2018 in minas gerais state brazil |
| topic | arboviruses yellow fever mosquitoes |
| url | https://www.mdpi.com/2075-4450/10/5/136 |
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