Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines
Cancer, a global health problem due to its increasing prevalence coupled with high mortality rate. Chemotherapy is an aggressive form of drug therapy. Herbal therapy is safer and healthier to treat specific medical issues. Nigella sativa has been used as traditional medicine for centuries. The crude...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Taibah University for Science |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2022.2161294 |
| _version_ | 1797215165295362048 |
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| author | Arusa Aftab Zubaida Yousaf Madiha Rashid Afifa Younas Hamna Yasin Nadia Riaz Qaisar Mansoor |
| author_facet | Arusa Aftab Zubaida Yousaf Madiha Rashid Afifa Younas Hamna Yasin Nadia Riaz Qaisar Mansoor |
| author_sort | Arusa Aftab |
| collection | DOAJ |
| description | Cancer, a global health problem due to its increasing prevalence coupled with high mortality rate. Chemotherapy is an aggressive form of drug therapy. Herbal therapy is safer and healthier to treat specific medical issues. Nigella sativa has been used as traditional medicine for centuries. The crude oil extracted from seed is effective against diseases but vegetative part of N. sativa has always been ignored for its therapeutic potential. Hence, it was hypothesized that vegetative part could be potential antineoplastic source against cancer. Therefore, reactive oxygen species determination and antineoplastic activity were performed against Hep2 and MCF7 cancer cell lines. n-butanol extract of Vegetative Part (VP) caused maximum cell death of Hep2 (58% cell viability). The chloroform VP extract showed 38% cell viability at 10 mg mL-1. The extracts with significant activity were later on validated through GCMS and LC-MS, following literature in PubChem, NIST and Spectrabase libraries. |
| first_indexed | 2024-03-10T08:13:44Z |
| format | Article |
| id | doaj.art-827e108047394680999cdf610b6d6993 |
| institution | Directory Open Access Journal |
| issn | 1658-3655 |
| language | English |
| last_indexed | 2024-04-24T11:25:44Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj.art-827e108047394680999cdf610b6d69932024-04-10T20:17:48ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552023-12-0117110.1080/16583655.2022.2161294Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell linesArusa Aftab0Zubaida Yousaf1Madiha Rashid2Afifa Younas3Hamna Yasin4Nadia Riaz5Qaisar Mansoor6Department of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanInstitute of Biomedical and Genetic Engineering, Islamabad, PakistanCancer, a global health problem due to its increasing prevalence coupled with high mortality rate. Chemotherapy is an aggressive form of drug therapy. Herbal therapy is safer and healthier to treat specific medical issues. Nigella sativa has been used as traditional medicine for centuries. The crude oil extracted from seed is effective against diseases but vegetative part of N. sativa has always been ignored for its therapeutic potential. Hence, it was hypothesized that vegetative part could be potential antineoplastic source against cancer. Therefore, reactive oxygen species determination and antineoplastic activity were performed against Hep2 and MCF7 cancer cell lines. n-butanol extract of Vegetative Part (VP) caused maximum cell death of Hep2 (58% cell viability). The chloroform VP extract showed 38% cell viability at 10 mg mL-1. The extracts with significant activity were later on validated through GCMS and LC-MS, following literature in PubChem, NIST and Spectrabase libraries.https://www.tandfonline.com/doi/10.1080/16583655.2022.2161294Antineoplasticcancercell linecytotoxicityGCMSphyto-medicine |
| spellingShingle | Arusa Aftab Zubaida Yousaf Madiha Rashid Afifa Younas Hamna Yasin Nadia Riaz Qaisar Mansoor Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines Journal of Taibah University for Science Antineoplastic cancer cell line cytotoxicity GCMS phyto-medicine |
| title | Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines |
| title_full | Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines |
| title_fullStr | Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines |
| title_full_unstemmed | Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines |
| title_short | Vegetative part of Nigella sativa L. potential antineoplastic sources against Hep2 and MCF7 human cancer cell lines |
| title_sort | vegetative part of nigella sativa l potential antineoplastic sources against hep2 and mcf7 human cancer cell lines |
| topic | Antineoplastic cancer cell line cytotoxicity GCMS phyto-medicine |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2022.2161294 |
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