Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms
There is a paucity of information regarding staphylococcal populations on teatcups of milking parlours in sheep and goat farms. The objectives were to describe the populations of staphylococci on teatcups in milking parlours in sheep or goat farms in two field investigations throughout Greece and to...
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MDPI AG
2021-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/4/385 |
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| author | Charalambia K. Michael Daphne T. Lianou Natalia G.C. Vasileiou Katerina Tsilipounidaki Angeliki I. Katsafadou Antonis P. Politis Nikos G. Kordalis Katerina S. Ioannidi Dimitris A. Gougoulis Constantina Trikalinou Denise C. Orfanou Ilektra A. Fragkou Panagiota I. Kontou Dimitra V. Liagka Vasia S. Mavrogianni Efthimia Petinaki George C. Fthenakis |
| author_facet | Charalambia K. Michael Daphne T. Lianou Natalia G.C. Vasileiou Katerina Tsilipounidaki Angeliki I. Katsafadou Antonis P. Politis Nikos G. Kordalis Katerina S. Ioannidi Dimitris A. Gougoulis Constantina Trikalinou Denise C. Orfanou Ilektra A. Fragkou Panagiota I. Kontou Dimitra V. Liagka Vasia S. Mavrogianni Efthimia Petinaki George C. Fthenakis |
| author_sort | Charalambia K. Michael |
| collection | DOAJ |
| description | There is a paucity of information regarding staphylococcal populations on teatcups of milking parlours in sheep and goat farms. The objectives were to describe the populations of staphylococci on teatcups in milking parlours in sheep or goat farms in two field investigations throughout Greece and to potentially associate the findings with the use of anti-staphylococcal mastitis vaccinations in the farms visited during the two investigations. In a cross-sectional (255 sheep and 66 goat farms across Greece) and a longitudinal (12 sheep farms, four samplings, throughout lactation) study, swab samples were collected from 1418 teatcups (upper and lower part) for staphylococcal recovery, identification and assessment of biofilm-formation. A total of 328 contaminated teatcups (23.1%) were found in 105 sheep (41.2%) and 35 goat (53.0%) farms. Staphylococci were more frequently recovered from the upper than the lower part of teatcups: 269 <i>versus</i> 139 teatcups, respectively. After identification, 253 staphylococcal isolates were found: <i>Staphylococcus aureus</i>, <i>Staphylococcus equorum</i>, <i>Staphylococcus lentus</i>, and <i>Staphylococcus capitis</i> predominated. Of these isolates, 87.4% were biofilm-forming. The proportion of contaminated teatcups was smaller in farms where vaccination against anti-staphylococcal mastitis in general or vaccination specifically against mastitis caused specifically by biofilm-forming staphylococcal strains was applied, 19.7% or 10.9%, respectively, <i>versus</i> 25.5% in farms without vaccination. In the longitudinal study, contaminated teatcups were identified in 28 (58.3%) sampling occasions, with staphylococci being recovered more frequently from their upper part. The same species as in the cross-sectional study predominated. Of these isolates, 61.9% were biofilm-forming. In farms where vaccination against mastitis caused specifically by biofilm-forming staphylococcal strains was applied, the proportion of contaminated teatcups was smaller: 20.4% <i>versus</i> 48.3% in farms without vaccination. There were no differences in proportions of contaminated teatcups between sampling occasions. In conclusion, the great majority of staphylococci recovered from teatcups of milking parlours in sheep and goat farms included biofilm-forming isolates. Reduced staphylococcal isolation was noted in farms where anti-staphylococcal vaccination was performed; this was possibly the effect of reduced excretion of staphylococci in the milk of vaccinated animals. |
| first_indexed | 2024-03-10T12:58:10Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T12:58:10Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj.art-8288ed49940f470c8ee85d7bf5a1c25a2023-11-21T11:46:44ZengMDPI AGPathogens2076-08172021-03-0110438510.3390/pathogens10040385Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat FarmsCharalambia K. Michael0Daphne T. Lianou1Natalia G.C. Vasileiou2Katerina Tsilipounidaki3Angeliki I. Katsafadou4Antonis P. Politis5Nikos G. Kordalis6Katerina S. Ioannidi7Dimitris A. Gougoulis8Constantina Trikalinou9Denise C. Orfanou10Ilektra A. Fragkou11Panagiota I. Kontou12Dimitra V. Liagka13Vasia S. Mavrogianni14Efthimia Petinaki15George C. Fthenakis16Veterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceFaculty of Animal Science, University of Thessaly, 41110 Larissa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceDepartment of Computer Science and Biomedical Informatics, University of Thessaly, 35131 Lamia, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital of Larissa, 41110 Larissa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceThere is a paucity of information regarding staphylococcal populations on teatcups of milking parlours in sheep and goat farms. The objectives were to describe the populations of staphylococci on teatcups in milking parlours in sheep or goat farms in two field investigations throughout Greece and to potentially associate the findings with the use of anti-staphylococcal mastitis vaccinations in the farms visited during the two investigations. In a cross-sectional (255 sheep and 66 goat farms across Greece) and a longitudinal (12 sheep farms, four samplings, throughout lactation) study, swab samples were collected from 1418 teatcups (upper and lower part) for staphylococcal recovery, identification and assessment of biofilm-formation. A total of 328 contaminated teatcups (23.1%) were found in 105 sheep (41.2%) and 35 goat (53.0%) farms. Staphylococci were more frequently recovered from the upper than the lower part of teatcups: 269 <i>versus</i> 139 teatcups, respectively. After identification, 253 staphylococcal isolates were found: <i>Staphylococcus aureus</i>, <i>Staphylococcus equorum</i>, <i>Staphylococcus lentus</i>, and <i>Staphylococcus capitis</i> predominated. Of these isolates, 87.4% were biofilm-forming. The proportion of contaminated teatcups was smaller in farms where vaccination against anti-staphylococcal mastitis in general or vaccination specifically against mastitis caused specifically by biofilm-forming staphylococcal strains was applied, 19.7% or 10.9%, respectively, <i>versus</i> 25.5% in farms without vaccination. In the longitudinal study, contaminated teatcups were identified in 28 (58.3%) sampling occasions, with staphylococci being recovered more frequently from their upper part. The same species as in the cross-sectional study predominated. Of these isolates, 61.9% were biofilm-forming. In farms where vaccination against mastitis caused specifically by biofilm-forming staphylococcal strains was applied, the proportion of contaminated teatcups was smaller: 20.4% <i>versus</i> 48.3% in farms without vaccination. There were no differences in proportions of contaminated teatcups between sampling occasions. In conclusion, the great majority of staphylococci recovered from teatcups of milking parlours in sheep and goat farms included biofilm-forming isolates. Reduced staphylococcal isolation was noted in farms where anti-staphylococcal vaccination was performed; this was possibly the effect of reduced excretion of staphylococci in the milk of vaccinated animals.https://www.mdpi.com/2076-0817/10/4/385biofilmgoatmastitismilking parloursheepstaphylococcus |
| spellingShingle | Charalambia K. Michael Daphne T. Lianou Natalia G.C. Vasileiou Katerina Tsilipounidaki Angeliki I. Katsafadou Antonis P. Politis Nikos G. Kordalis Katerina S. Ioannidi Dimitris A. Gougoulis Constantina Trikalinou Denise C. Orfanou Ilektra A. Fragkou Panagiota I. Kontou Dimitra V. Liagka Vasia S. Mavrogianni Efthimia Petinaki George C. Fthenakis Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms Pathogens biofilm goat mastitis milking parlour sheep staphylococcus |
| title | Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms |
| title_full | Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms |
| title_fullStr | Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms |
| title_full_unstemmed | Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms |
| title_short | Association of Staphylococcal Populations on Teatcups of Milking Parlours with Vaccination against Staphylococcal Mastitis in Sheep and Goat Farms |
| title_sort | association of staphylococcal populations on teatcups of milking parlours with vaccination against staphylococcal mastitis in sheep and goat farms |
| topic | biofilm goat mastitis milking parlour sheep staphylococcus |
| url | https://www.mdpi.com/2076-0817/10/4/385 |
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