Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses
The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, <i>Candida albicans</i> infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/8/1602 |
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| author | Jong-Kook Lee Soyoung Park Young-Min Kim Taeuk Guk Min-Young Lee Seong-Cheol Park Jung Ro Lee Mi-Kyeong Jang |
| author_facet | Jong-Kook Lee Soyoung Park Young-Min Kim Taeuk Guk Min-Young Lee Seong-Cheol Park Jung Ro Lee Mi-Kyeong Jang |
| author_sort | Jong-Kook Lee |
| collection | DOAJ |
| description | The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, <i>Candida albicans</i> infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant <i>C. albicans</i>. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant <i>C. albicans</i> cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses. |
| first_indexed | 2024-03-09T03:58:36Z |
| format | Article |
| id | doaj.art-828b74fb5811438db0150f051a25cc38 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T03:58:36Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-828b74fb5811438db0150f051a25cc382023-12-03T14:17:12ZengMDPI AGPharmaceutics1999-49232022-07-01148160210.3390/pharmaceutics14081602Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact LensesJong-Kook Lee0Soyoung Park1Young-Min Kim2Taeuk Guk3Min-Young Lee4Seong-Cheol Park5Jung Ro Lee6Mi-Kyeong Jang7Department of Chemical Engineering, Sunchon National University, Suncheon 57922, KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, KoreaDepartment of Clinical Laboratory Science, Daejeon Health Institute of Technology, Daejeon 34504, KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, KoreaLMO Team, National Institute of Ecology (NIE), Seocheon 33657, KoreaDepartment of Chemical Engineering, Sunchon National University, Suncheon 57922, KoreaThe recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, <i>Candida albicans</i> infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant <i>C. albicans</i>. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant <i>C. albicans</i> cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses.https://www.mdpi.com/1999-4923/14/8/1602antifungal activityantimicrobial peptidecandidacidal activitybiofilm |
| spellingShingle | Jong-Kook Lee Soyoung Park Young-Min Kim Taeuk Guk Min-Young Lee Seong-Cheol Park Jung Ro Lee Mi-Kyeong Jang Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses Pharmaceutics antifungal activity antimicrobial peptide candidacidal activity biofilm |
| title | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses |
| title_full | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses |
| title_fullStr | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses |
| title_full_unstemmed | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses |
| title_short | Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant <i>Candida albicans</i> on Contact Lenses |
| title_sort | candidacidal and antibiofilm activity of ps1 3 peptide against drug resistant i candida albicans i on contact lenses |
| topic | antifungal activity antimicrobial peptide candidacidal activity biofilm |
| url | https://www.mdpi.com/1999-4923/14/8/1602 |
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