Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase
Abstract Background The QX-type infectious bronchitis virus (IBV) has become the predominant genotype worldwide in recent years and has caused serious economic losses to the chicken industry. The most significant feature of QX IBV is that its infection in the early growing stage can cause abnormal o...
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| Format: | Article |
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BMC
2022-04-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-022-01788-0 |
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| author | Xiaorong Zhang Kun Yan Chengcheng Zhang Mengjiao Guo Shuqin Chen Kai Liao Zongyi Bo Yongzhong Cao Yantao Wu |
| author_facet | Xiaorong Zhang Kun Yan Chengcheng Zhang Mengjiao Guo Shuqin Chen Kai Liao Zongyi Bo Yongzhong Cao Yantao Wu |
| author_sort | Xiaorong Zhang |
| collection | DOAJ |
| description | Abstract Background The QX-type infectious bronchitis virus (IBV) has become the predominant genotype worldwide in recent years and has caused serious economic losses to the chicken industry. The most significant feature of QX IBV is that its infection in the early growing stage can cause abnormal oviduct development, resulting in a high proportion of ‘false layers’ in poultry flocks of laying hens and breeders. However, few studies have evaluated whether infections of QX-type IBV in laying stages can also cause severe pathological changes in the oviduct. Methods In this study, 300-day-old specific-pathogen-free chickens were infected either with the QX-type strain QXL or Massachusetts (Mass)-type strain M41 to compare their pathogenicity on different segments of the oviduct. Results Both the QXL and M41 strains successfully replicated in all segments of the oviduct; however, the QXL strain was more highly distributed in mucosal layer and caused severe lesions in the lamina propria, including interstitial dilation, inflammatory cell infiltration, and distinct expansion of tubular glands. Moreover, the QXL strain induced high expression of proinflammatory cytokines and cytotoxic molecules in the majority of segments in the oviduct. Further research found that the QXL strain may affected the formation of shell membranes and eggshells by inhibiting the expression of type I collagen and CaBP-D28k. Conclusions Our results indicate that the QX-type IBV is more pathogenic than Mass-type IBV to oviduct in laying phase. Collectively, these findings provide detailed information on the pathological changes in different segments of the oviduct in laying phase, which could offer a better understanding about the pathogenicity of IBV. |
| first_indexed | 2024-12-21T14:43:22Z |
| format | Article |
| id | doaj.art-82923563efdf47c899d4260afad293b9 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-21T14:43:22Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-82923563efdf47c899d4260afad293b92022-12-21T19:00:07ZengBMCVirology Journal1743-422X2022-04-0119111010.1186/s12985-022-01788-0Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phaseXiaorong Zhang0Kun Yan1Chengcheng Zhang2Mengjiao Guo3Shuqin Chen4Kai Liao5Zongyi Bo6Yongzhong Cao7Yantao Wu8Jiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityThe International Joint Research Laboratory of Agricultural and Agri-Product Safety, Yangzhou UniversityThe International Joint Research Laboratory of Agricultural and Agri-Product Safety, Yangzhou UniversityJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityAbstract Background The QX-type infectious bronchitis virus (IBV) has become the predominant genotype worldwide in recent years and has caused serious economic losses to the chicken industry. The most significant feature of QX IBV is that its infection in the early growing stage can cause abnormal oviduct development, resulting in a high proportion of ‘false layers’ in poultry flocks of laying hens and breeders. However, few studies have evaluated whether infections of QX-type IBV in laying stages can also cause severe pathological changes in the oviduct. Methods In this study, 300-day-old specific-pathogen-free chickens were infected either with the QX-type strain QXL or Massachusetts (Mass)-type strain M41 to compare their pathogenicity on different segments of the oviduct. Results Both the QXL and M41 strains successfully replicated in all segments of the oviduct; however, the QXL strain was more highly distributed in mucosal layer and caused severe lesions in the lamina propria, including interstitial dilation, inflammatory cell infiltration, and distinct expansion of tubular glands. Moreover, the QXL strain induced high expression of proinflammatory cytokines and cytotoxic molecules in the majority of segments in the oviduct. Further research found that the QXL strain may affected the formation of shell membranes and eggshells by inhibiting the expression of type I collagen and CaBP-D28k. Conclusions Our results indicate that the QX-type IBV is more pathogenic than Mass-type IBV to oviduct in laying phase. Collectively, these findings provide detailed information on the pathological changes in different segments of the oviduct in laying phase, which could offer a better understanding about the pathogenicity of IBV.https://doi.org/10.1186/s12985-022-01788-0Infectious bronchitis virusQX-typePathogenicityOviductLaying phaseEgg quality |
| spellingShingle | Xiaorong Zhang Kun Yan Chengcheng Zhang Mengjiao Guo Shuqin Chen Kai Liao Zongyi Bo Yongzhong Cao Yantao Wu Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase Virology Journal Infectious bronchitis virus QX-type Pathogenicity Oviduct Laying phase Egg quality |
| title | Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase |
| title_full | Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase |
| title_fullStr | Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase |
| title_full_unstemmed | Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase |
| title_short | Pathogenicity comparison between QX-type and Mass-type infectious bronchitis virus to different segments of the oviducts in laying phase |
| title_sort | pathogenicity comparison between qx type and mass type infectious bronchitis virus to different segments of the oviducts in laying phase |
| topic | Infectious bronchitis virus QX-type Pathogenicity Oviduct Laying phase Egg quality |
| url | https://doi.org/10.1186/s12985-022-01788-0 |
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