Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae)
Premise of the study: New microsatellite (simple sequence repeat [SSR]) primers were developed from <i>Eucalyptus</i> expressed sequence tags (ESTs) and optimized for genetic studies of the southwestern Australian tree <i>E. gomphocephala</i>, which is severely impacted by tr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-07-01
|
| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300004 |
| _version_ | 1818482200219222016 |
|---|---|
| author | Donna Bradbury Ann Smithson Siegfried L. Krauss |
| author_facet | Donna Bradbury Ann Smithson Siegfried L. Krauss |
| author_sort | Donna Bradbury |
| collection | DOAJ |
| description | Premise of the study: New microsatellite (simple sequence repeat [SSR]) primers were developed from <i>Eucalyptus</i> expressed sequence tags (ESTs) and optimized for genetic studies of the southwestern Australian tree <i>E. gomphocephala</i>, which is severely impacted by tree health decline and habitat fragmentation.
Methods and Results: A total of 133 gene-homologous EST-SSR primer pairs were designed for <i>Eucalyptus</i>, and 44 were screened in <i>E. gomphocephala</i>. Of these, 17 produced reliable amplification products and 11 were polymorphic. Between two and 13 alleles were observed per locus, and observed heterozygosities ranged from 0.172 to 0.867. All 17 EST-SSRs that amplified <i>E. gomphocephala</i> cross-amplified to at least one of <i>E. marginata</i>, <i>E. camaldulensis</i>, and <i>E. victrix</i>.
Conclusions: This set of EST-SSR primer pairs will be valuable tools for future population genetic studies of <i>E. gomphocephala</i> and other eucalypts, particularly for studying gene-linked variation and informing seed-sourcing strategies for ecological restoration. |
| first_indexed | 2024-12-10T11:44:35Z |
| format | Article |
| id | doaj.art-82959bde28cf4081ab595e49ebe557da |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-10T11:44:35Z |
| publishDate | 2013-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-82959bde28cf4081ab595e49ebe557da2022-12-22T01:50:07ZengWileyApplications in Plant Sciences2168-04502013-07-0118130000410.3732/apps.1300004Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae)Donna Bradbury0Ann Smithson1Siegfried L. Krauss2School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6005, Australia; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, Fraser Avenue, West Perth, Western Australia 6009, AustraliaSchool of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6005, Australia; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, Fraser Avenue, West Perth, Western Australia 6009, AustraliaSchool of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6005, Australia; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, Fraser Avenue, West Perth, Western Australia 6009, AustraliaPremise of the study: New microsatellite (simple sequence repeat [SSR]) primers were developed from <i>Eucalyptus</i> expressed sequence tags (ESTs) and optimized for genetic studies of the southwestern Australian tree <i>E. gomphocephala</i>, which is severely impacted by tree health decline and habitat fragmentation. Methods and Results: A total of 133 gene-homologous EST-SSR primer pairs were designed for <i>Eucalyptus</i>, and 44 were screened in <i>E. gomphocephala</i>. Of these, 17 produced reliable amplification products and 11 were polymorphic. Between two and 13 alleles were observed per locus, and observed heterozygosities ranged from 0.172 to 0.867. All 17 EST-SSRs that amplified <i>E. gomphocephala</i> cross-amplified to at least one of <i>E. marginata</i>, <i>E. camaldulensis</i>, and <i>E. victrix</i>. Conclusions: This set of EST-SSR primer pairs will be valuable tools for future population genetic studies of <i>E. gomphocephala</i> and other eucalypts, particularly for studying gene-linked variation and informing seed-sourcing strategies for ecological restoration.http://www.bioone.org/doi/full/10.3732/apps.1300004ecologically important genetic variationEST-microsatelliteEucalyptus gomphocephalaMyrtaceaeSSR miningtuart |
| spellingShingle | Donna Bradbury Ann Smithson Siegfried L. Krauss Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae) Applications in Plant Sciences ecologically important genetic variation EST-microsatellite Eucalyptus gomphocephala Myrtaceae SSR mining tuart |
| title | Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae) |
| title_full | Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae) |
| title_fullStr | Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae) |
| title_full_unstemmed | Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae) |
| title_short | Development and Testing of New Gene-Homologous EST-SSRs for <i>Eucalyptus gomphocephala</i> (Myrtaceae) |
| title_sort | development and testing of new gene homologous est ssrs for i eucalyptus gomphocephala i myrtaceae |
| topic | ecologically important genetic variation EST-microsatellite Eucalyptus gomphocephala Myrtaceae SSR mining tuart |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300004 |
| work_keys_str_mv | AT donnabradbury developmentandtestingofnewgenehomologousestssrsforieucalyptusgomphocephalaimyrtaceae AT annsmithson developmentandtestingofnewgenehomologousestssrsforieucalyptusgomphocephalaimyrtaceae AT siegfriedlkrauss developmentandtestingofnewgenehomologousestssrsforieucalyptusgomphocephalaimyrtaceae |