WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers
<em><strong>Objective(s):</strong> </em>Breast cancer is the second leading cause of cancer death in females. Understanding molecular mechanisms in cancer cells compared with normal cells is crucial for diagnostic and therapeutic strategies. Long intergenic non-protein coding...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2019-11-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_13881_c2a8fd723d6beb1fe2825eb5bef64ca2.pdf |
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| author | Sara Kor vahid Erfani-Moghadam Reza Sahebi Shabbou Bahramian Mohammad Shafiee |
| author_facet | Sara Kor vahid Erfani-Moghadam Reza Sahebi Shabbou Bahramian Mohammad Shafiee |
| author_sort | Sara Kor |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong> </em>Breast cancer is the second leading cause of cancer death in females. Understanding molecular mechanisms in cancer cells compared with normal cells is crucial for diagnostic and therapeutic strategies. Long intergenic non-protein coding RNA, a regulator of reprogramming (lincRNA-RoR) is a noncoding RNA which initially was detected in induced pluripotent stem cells, and it has an important role in cell reprogramming and highly expressed in breast cancer cells. A key point in successful gene silencing is the usage of siRNA delivery system that is safe and efficient. <br /><em><strong>Materials and Methods:</strong></em> In this study, the fifth-generation of PAMAM dendrimer is used as a nanocarrier for entering siRNA molecules for gene silencing of lincRNA-RoR. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle. Gel retardation assay was used to find the best Negative/Positive (N/P) molar charge ratio of siRNA- PAMAM transfected into MDA-MB 231 cells. MTT assay was performed 24 hr after transfection revealed the IC50 value (half maximal inhibitory concentrations) about 100 nanomolar for lincRNA-ROR siRNA.<br /><em><strong>Results:</strong></em> The lincRNA-RoR and WDR7 gene expression changes were evaluated by real-time PCR after siRNA treatment and showed an increase in the gene expression of WDR7. <br /><em><strong>Conclusion:</strong></em> This study showed that PAMAM dendrimer G5/ siRNA could be a useful system delivery for future gene therapy approaches. |
| first_indexed | 2024-04-13T08:03:37Z |
| format | Article |
| id | doaj.art-82970b0f885c4f97b055f847ce6c3a58 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T08:03:37Z |
| publishDate | 2019-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-82970b0f885c4f97b055f847ce6c3a582022-12-22T02:55:14ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742019-11-0122111283128710.22038/ijbms.2019.36135.860713881WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimersSara Kor0vahid Erfani-Moghadam1Reza Sahebi2Shabbou Bahramian3Mohammad Shafiee4Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, IranMedical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran|Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, IranDepartment of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranStem Cell Research Center, Golestan University of Medical Sciences, Gorgan, IranStem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran|Department of Medical Genetics, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran<em><strong>Objective(s):</strong> </em>Breast cancer is the second leading cause of cancer death in females. Understanding molecular mechanisms in cancer cells compared with normal cells is crucial for diagnostic and therapeutic strategies. Long intergenic non-protein coding RNA, a regulator of reprogramming (lincRNA-RoR) is a noncoding RNA which initially was detected in induced pluripotent stem cells, and it has an important role in cell reprogramming and highly expressed in breast cancer cells. A key point in successful gene silencing is the usage of siRNA delivery system that is safe and efficient. <br /><em><strong>Materials and Methods:</strong></em> In this study, the fifth-generation of PAMAM dendrimer is used as a nanocarrier for entering siRNA molecules for gene silencing of lincRNA-RoR. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle. Gel retardation assay was used to find the best Negative/Positive (N/P) molar charge ratio of siRNA- PAMAM transfected into MDA-MB 231 cells. MTT assay was performed 24 hr after transfection revealed the IC50 value (half maximal inhibitory concentrations) about 100 nanomolar for lincRNA-ROR siRNA.<br /><em><strong>Results:</strong></em> The lincRNA-RoR and WDR7 gene expression changes were evaluated by real-time PCR after siRNA treatment and showed an increase in the gene expression of WDR7. <br /><em><strong>Conclusion:</strong></em> This study showed that PAMAM dendrimer G5/ siRNA could be a useful system delivery for future gene therapy approaches.http://ijbms.mums.ac.ir/article_13881_c2a8fd723d6beb1fe2825eb5bef64ca2.pdfbreast cancerlncrna rorpolyamidoamine dendrimer (pamam)transfectionwdr7 |
| spellingShingle | Sara Kor vahid Erfani-Moghadam Reza Sahebi Shabbou Bahramian Mohammad Shafiee WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers Iranian Journal of Basic Medical Sciences breast cancer lncrna ror polyamidoamine dendrimer (pamam) transfection wdr7 |
| title | WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers |
| title_full | WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers |
| title_fullStr | WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers |
| title_full_unstemmed | WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers |
| title_short | WDR7 up-regulation upon knocking down of neighboring non-coding RNA using siRNAs encapsulated in polyamidoamine dendrimers |
| title_sort | wdr7 up regulation upon knocking down of neighboring non coding rna using sirnas encapsulated in polyamidoamine dendrimers |
| topic | breast cancer lncrna ror polyamidoamine dendrimer (pamam) transfection wdr7 |
| url | http://ijbms.mums.ac.ir/article_13881_c2a8fd723d6beb1fe2825eb5bef64ca2.pdf |
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