High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat
A rapid and sensitive high performance liquid chromatographic method has been developed for the determination of diclofenac sodium in rat plasma. The assay was performed after liquid-liquid extraction with 1M orthophosphoric acid and a mixture of hexane:isopropyl alcohol. Chromatographic separations...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2013-12-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://www.vetmedmosul.org/ijvs/media/13-2-6e.pdf |
| Summary: | A rapid and sensitive high performance liquid chromatographic method has been developed for the determination of diclofenac sodium in rat plasma. The assay was performed after liquid-liquid extraction with 1M orthophosphoric acid and a mixture of hexane:isopropyl alcohol. Chromatographic separations were performed on C18 stationary phase with a mobile phase composed of acetonitrile: deionised water: orthophosphoric acid (45:54.5:0.5,v/v) as mobile phase with final pH of (3.5). Analytes were detected at wave length of 276nm. This method was validated for specificity and linearity with a correlation coefficient, r=0.99. |
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| ISSN: | 1607-3894 2071-1255 |