High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat
A rapid and sensitive high performance liquid chromatographic method has been developed for the determination of diclofenac sodium in rat plasma. The assay was performed after liquid-liquid extraction with 1M orthophosphoric acid and a mixture of hexane:isopropyl alcohol. Chromatographic separations...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2013-12-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://www.vetmedmosul.org/ijvs/media/13-2-6e.pdf |
| _version_ | 1828381945784958976 |
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| author | Z.S. Hamad B.M. Yahya |
| author_facet | Z.S. Hamad B.M. Yahya |
| author_sort | Z.S. Hamad |
| collection | DOAJ |
| description | A rapid and sensitive high performance liquid chromatographic method has been developed for the determination of diclofenac sodium in rat plasma. The assay was performed after liquid-liquid extraction with 1M orthophosphoric acid and a mixture of hexane:isopropyl alcohol. Chromatographic separations were performed on C18 stationary phase with a mobile phase composed of acetonitrile: deionised water: orthophosphoric acid (45:54.5:0.5,v/v) as mobile phase with final pH of (3.5). Analytes were detected at wave length of 276nm. This method was validated for specificity and linearity with a correlation coefficient, r=0.99. |
| first_indexed | 2024-12-10T04:24:08Z |
| format | Article |
| id | doaj.art-82990fdf1ff04e37a9333d55a596303d |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-10T04:24:08Z |
| publishDate | 2013-12-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-82990fdf1ff04e37a9333d55a596303d2022-12-22T02:02:19ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552013-12-01272103107High performance liquid chromatographic determination of diclofenac sodium in plasma of the ratZ.S. Hamad 0B.M. Yahya1Department of Pharmacology, College of Pharmacy, University of Mosul, IraqDepartment of Pharmacology, College of Pharmacy, University of Mosul, IraqA rapid and sensitive high performance liquid chromatographic method has been developed for the determination of diclofenac sodium in rat plasma. The assay was performed after liquid-liquid extraction with 1M orthophosphoric acid and a mixture of hexane:isopropyl alcohol. Chromatographic separations were performed on C18 stationary phase with a mobile phase composed of acetonitrile: deionised water: orthophosphoric acid (45:54.5:0.5,v/v) as mobile phase with final pH of (3.5). Analytes were detected at wave length of 276nm. This method was validated for specificity and linearity with a correlation coefficient, r=0.99.http://www.vetmedmosul.org/ijvs/media/13-2-6e.pdf: Liquid chromatographicDiclofenac sodiumRat |
| spellingShingle | Z.S. Hamad B.M. Yahya High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat Iraqi Journal of Veterinary Sciences : Liquid chromatographic Diclofenac sodium Rat |
| title | High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat |
| title_full | High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat |
| title_fullStr | High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat |
| title_full_unstemmed | High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat |
| title_short | High performance liquid chromatographic determination of diclofenac sodium in plasma of the rat |
| title_sort | high performance liquid chromatographic determination of diclofenac sodium in plasma of the rat |
| topic | : Liquid chromatographic Diclofenac sodium Rat |
| url | http://www.vetmedmosul.org/ijvs/media/13-2-6e.pdf |
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